Difference between revisions of "Team:CU Boulder"

 
(125 intermediate revisions by the same user not shown)
Line 4: Line 4:
 
<style>
 
<style>
  
 +
div {
 +
    padding: 0px;
 +
    margin: 0px;
 +
}
 +
#abstract {
 +
    margin-bottom: 100px;
 +
}
 
#abstract h1 {
 
#abstract h1 {
 
     font-size: 40px;
 
     font-size: 40px;
Line 9: Line 16:
 
#abstract p {
 
#abstract p {
 
     font-size: 24px;
 
     font-size: 24px;
 +
}
 +
.margins {
 +
    width: 80%;
 +
    min-width: 780px;
 +
    max-width: 1000px; /* Vertical margins, and auto to center */
 +
    margin: auto;
 +
    margin-top: 0px;
 +
/*border: 3px solid red;*/
 +
    padding: 0px;
 
}
 
}
 
#stripe_1 {
 
#stripe_1 {
 
     width: 100%;
 
     width: 100%;
−
     height: 300px;
+
     height: 350px;
−
     background-color: blue;
+
     background-color: #9AE9F6;
 +
    padding-bottom: 0px;
 +
    z-index: 1;
 +
}
 +
.image {
 +
    float: right;
 +
    background-repeat: no-repeat;
 +
    background-image: url(https://static.igem.org/mediawiki/2014/1/18/CU_Boulder_factory.gif);
 +
    height: 250px;
 +
    width: 200px;
 
}
 
}
 
#stripe_2 {
 
#stripe_2 {
 
     width: 100%;
 
     width: 100%;
 
     height: 400px;
 
     height: 400px;
−
     background-color: gray;  
+
     background-color: #4B5159;
 +
    z-index: 10;
 +
 
 +
  -webkit-box-shadow: -1px 1px 5px 5px rgba(50, 50, 50, 0.5);
 +
  -moz-box-shadow:    -1px 1px 30px 100px rgba(50, 50, 50, 0.5);
 +
    box-shadow: 0px 30px 100px #505050;
 
}
 
}
 +
 +
div h2 {
 +
    text-transform: uppercase;
 +
    /*text-shadow: 2px 2px 2px #CCCCCC;*/
 +
    font-size: 38px;
 +
    margin-left: 10px;
 +
    /*margin: 0px;*/
 +
    padding: 0px;
 +
    /*font-family: "Times New Roman",Times,serif;*/
 +
    font-family: "Trebuchet MS", Helvetica, sans-serif;
 +
    font-weight: lighter;
 +
    letter-spacing: .4px;
 +
    text-align: center;
 +
    line-height: 100%;
 +
}
 +
#CU_Naph {
 +
    float: left;
 +
/*border: 3px solid blue;*/
 +
    vertical-align: middle
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    width: 560px;
 +
}
 +
div h3 {
 +
    text-transform: uppercase;
 +
    font-size: 88px;
 +
    font-family: Impact, Charcoal, sans-serif;
 +
    font-weight: lighter;
 +
    text-align: center;
 +
    color: #9AE9F6;
 +
    line-height: 165%;
 +
}
 +
div h4 {
 +
    text-transform: uppercase;
 +
    font-size: 70px;
 +
    font-family: "Trebuchet MS", Helvetica, sans-serif;
 +
    font-weight: heavy;
 +
    text-align: center;
 +
    letter-spacing: .4px;
 +
    line-height: 130%;
 +
}
 +
 +
#quick_nav {
 +
    height: 450px;
 +
    background-color: #989898;
 +
}
 +
#quick_nav_container {
 +
    width: 800px;
 +
    height: 320px;
 +
    /*border: 2px solid purple;*/
 +
    /*padding-left: 40px;*/
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    padding-top: 50px;
 +
}
 +
.quick_nav_box {
 +
    height: 300px;
 +
    width: 240px;
 +
    vertical-align: middle;
 +
    margin-left: 10px;
 +
    margin-right: 10px;
 +
    background-color: gray;
 +
    /*border: 2px solid red;*/
 +
    float: left;
 +
 +
}
 +
#nav_box1 {
 +
    float: left;
 +
}
 +
#nav_box2 {
 +
    float: left;
 +
}
 +
#nav_box3 {
 +
    float: right;
 +
  /* background-image: url(https://static.igem.org/mediawiki/2015/b/b3/CU_Boulder_flower.gif);
 +
    background-size: auto 100%;*/
 +
}
 +
h5 {
 +
    font-size: 30px;
 +
    vertical-align: middle;
 +
    text-align: center;
 +
    margin-top: 20px;
 +
}
 +
#quick_nav_container h5 a{
 +
    color: black;
 +
}
 +
#quick_nav_container h5 a:link {
 +
    color: black;
 +
}
 +
  
  
Line 31: Line 151:
  
 
<!----------- front image ------------->
 
<!----------- front image ------------->
−
<div style="text-align: right">
+
 
−
     <img src="https://static.igem.org/mediawiki/2014/1/18/CU_Boulder_factory.gif" text-align="center" alt="CU-Boulder"/>
+
<div id="stripe_1">
 +
<div class="margins">
 +
     <div id="CU_Naph">
 +
        <h2>UNIVERSITY OF COLORADO</h2>
 +
        <h2>NAPHTHALENE RECORDERS</h2>
 +
    </div>
 +
    <div class="image"></div>
 +
</div>
 
</div>
 
</div>
  
 +
<div id="stripe_2">
 +
<div class="margins">
 +
    <br><br>
 +
    <br>
 +
    <h3>A Big Fracking Deal</h3>
 +
    <!--<h4>A Big Fracking Deal</h4>-->
 +
</div>
 +
</div>
 +
 +
<div id="quick_nav">
 +
<div id="quick_nav_container">
 +
    <div class="quick_nav_box" id=nav_box1">
 +
        <h5><a href="https://2015.igem.org/Team:CU_Boulder/project/motivation">Problem</a></h5>
 +
    </div>
 +
    <div class="quick_nav_box" id=nav_box2">
 +
        <h5><a href="https://2015.igem.org/Team:CU_Boulder/Description">Solution</a></h5>
 +
    </div>
 +
    <div class="quick_nav_box" id=nav_box3">
 +
        <h5><a href="https://2015.igem.org/Team:CU_Boulder/project/results">Results</a></h5>
 +
    </div>
 +
 +
</div>
 +
</div>
  
  
Line 42: Line 192:
 
   
 
   
  
−
 
+
 
−
<div id="abstract"<div>
+
<div class="margins"> 
 +
<div id="abstract">
 
<h1>Abstract</h1>
 
<h1>Abstract</h1>
  
 
<p>Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds.  In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water.  To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking.  Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase.  Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.</p>
 
<p>Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds.  In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water.  To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking.  Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase.  Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.</p>
  
 +
</div>
 
</div>
 
</div>
  

Latest revision as of 04:48, 18 September 2015

File:Example.jpg


<!DOCTYPE html> Team:CU_Boulder - 2015.igem.org

UNIVERSITY OF COLORADO

NAPHTHALENE RECORDERS




A Big Fracking Deal

Abstract

Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds. In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water. To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking. Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase. Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.

Team:CU-Boulder - 2015.igem.org