Difference between revisions of "Team:CU Boulder"

 
(141 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
{{CU_Boulder}}
+
[[File:Example.jpg]]{{CU_Boulder}}
 
<html>
 
<html>
−
<article>
+
<head>
−
    <!-- front image -->
+
<style>
−
<div style="text-align: center">
+
 
−
<img src="https://static.igem.org/mediawiki/2015/6/6b/CU-Boulder-temp-cu-photo.gif" text-align="center" alt="CU-Boulder"/>
+
div {
 +
    padding: 0px;
 +
    margin: 0px;
 +
}
 +
#abstract {
 +
    margin-bottom: 100px;
 +
}
 +
#abstract h1 {
 +
    font-size: 40px;
 +
}
 +
#abstract p {
 +
    font-size: 24px;
 +
}
 +
.margins {
 +
    width: 80%;
 +
    min-width: 780px;
 +
    max-width: 1000px; /* Vertical margins, and auto to center */
 +
    margin: auto;
 +
    margin-top: 0px;
 +
/*border: 3px solid red;*/
 +
    padding: 0px;
 +
}
 +
#stripe_1 {
 +
    width: 100%;
 +
    height: 350px;
 +
    background-color: #9AE9F6;
 +
    padding-bottom: 0px;
 +
    z-index: 1;
 +
}
 +
.image {
 +
    float: right;
 +
    background-repeat: no-repeat;
 +
    background-image: url(https://static.igem.org/mediawiki/2014/1/18/CU_Boulder_factory.gif);
 +
    height: 250px;
 +
    width: 200px;
 +
}
 +
#stripe_2 {
 +
    width: 100%;
 +
    height: 400px;
 +
    background-color: #4B5159;
 +
    z-index: 10;
 +
 
 +
  -webkit-box-shadow: -1px 1px 5px 5px rgba(50, 50, 50, 0.5);
 +
  -moz-box-shadow:    -1px 1px 30px 100px rgba(50, 50, 50, 0.5);
 +
    box-shadow: 0px 30px 100px #505050;
 +
}
 +
 
 +
div h2 {
 +
    text-transform: uppercase;
 +
    /*text-shadow: 2px 2px 2px #CCCCCC;*/
 +
    font-size: 38px;
 +
    margin-left: 10px;
 +
    /*margin: 0px;*/
 +
    padding: 0px;
 +
    /*font-family: "Times New Roman",Times,serif;*/
 +
    font-family: "Trebuchet MS", Helvetica, sans-serif;
 +
    font-weight: lighter;
 +
    letter-spacing: .4px;
 +
    text-align: center;
 +
    line-height: 100%;
 +
}
 +
#CU_Naph {
 +
    float: left;
 +
/*border: 3px solid blue;*/
 +
    vertical-align: middle
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    width: 560px;
 +
}
 +
div h3 {
 +
    text-transform: uppercase;
 +
    font-size: 88px;
 +
    font-family: Impact, Charcoal, sans-serif;
 +
    font-weight: lighter;
 +
    text-align: center;
 +
    color: #9AE9F6;
 +
    line-height: 165%;
 +
}
 +
div h4 {
 +
    text-transform: uppercase;
 +
    font-size: 70px;
 +
    font-family: "Trebuchet MS", Helvetica, sans-serif;
 +
    font-weight: heavy;
 +
    text-align: center;
 +
    letter-spacing: .4px;
 +
    line-height: 130%;
 +
}
 +
 
 +
#quick_nav {
 +
    height: 450px;
 +
    background-color: #989898;
 +
}
 +
#quick_nav_container {
 +
    width: 800px;
 +
    height: 320px;
 +
    /*border: 2px solid purple;*/
 +
    /*padding-left: 40px;*/
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    padding-top: 50px;
 +
}
 +
.quick_nav_box {
 +
    height: 300px;
 +
    width: 240px;
 +
    vertical-align: middle;
 +
    margin-left: 10px;
 +
    margin-right: 10px;
 +
    background-color: gray;
 +
    /*border: 2px solid red;*/
 +
    float: left;
 +
 
 +
}
 +
#nav_box1 {
 +
    float: left;
 +
}
 +
#nav_box2 {
 +
    float: left;
 +
}
 +
#nav_box3 {
 +
    float: right;
 +
  /* background-image: url(https://static.igem.org/mediawiki/2015/b/b3/CU_Boulder_flower.gif);
 +
    background-size: auto 100%;*/
 +
}
 +
h5 {
 +
    font-size: 30px;
 +
    vertical-align: middle;
 +
    text-align: center;
 +
    margin-top: 20px;
 +
}
 +
#quick_nav_container h5 a{
 +
    color: black;
 +
}
 +
#quick_nav_container h5 a:link {
 +
    color: black;
 +
}
 +
 
 +
 
 +
 
 +
</style>
 +
 
 +
</head>
 +
<body>
 +
 
 +
 
 +
<div>
 +
 
 +
<!----------- front image ------------->
 +
 
 +
<div id="stripe_1">
 +
<div class="margins">
 +
    <div id="CU_Naph">
 +
        <h2>UNIVERSITY OF COLORADO</h2>
 +
        <h2>NAPHTHALENE RECORDERS</h2>
 
     </div>
 
     </div>
−
<p></p>
+
    <div class="image"></div>
−
<h1>Abstract</h1>
+
</div>
−
        <p class="box-float-right">I am a sample box. I hold content and look, I have a shadow. I am also personified for some reason. I am not as awesome as the Fact Sphere.</p>
+
</div>
−
    <p>The <abbr rel="glossary" title="A very intelligent, handsome, and factually correct spherical robot">Fact Sphere</abbr> is the most intelligent sphere. The Fact Sphere is the most handsome sphere. The Fact Sphere is incredibly handsome. The Fact Sphere is always right. The Adventure Sphere is a blowhard and a coward. The Space Sphere will never go to spacer. Fact: Space does not exist. Spheres that insist on going into space are inferior to spheres that don’t. The Fact Sphere is a good person, whose insights are relevant. The Fact Sphere is a good sphere, with many friends. Whoever wins this battle is clearly superior, and will earn the allegiance of the Fact Sphere.</p>
+
−
    <p>Pants were invented by sailors in the sixteenth century to avoid Poseidon’s wrath. It was believed that the sight of naked sailors angered the sea god. 89% of magic tricks are not magic. Technically, they are sorcery. Polymerase I polypeptide A is a human gene. The Schrodinger’s cat paradox outlines a situation in which a <abbr rel="glossary" title="Soft kitty, warm kitty, little ball of fur....">cat</abbr> in a box must be considered, for all intents and purposes, simultaneously alive and dead. Schrodinger created this paradox as a justification for killing cats.</p>
+
−
<p>It is incorrectly noted that Thomas Edison invented ‘push-ups’ in 1878. Nikolai Tesla had in fact patented the activity three years earlier, under the name ‘Tesla-cize’. Whales are twice as intelligent, and three times as delicious as humans. The first person to prove that cow’s milk is drinkable was very, very thirsty.</p>
+
  
−
<!-- citation: http://theportalwiki.com/wiki/Core_voice_lines -->      
+
<div id="stripe_2">
 +
<div class="margins">
 +
    <br><br>
 +
    <br>
 +
    <h3>A Big Fracking Deal</h3>
 +
    <!--<h4>A Big Fracking Deal</h4>-->
 +
</div>
 +
</div>
 +
 
 +
<div id="quick_nav">
 +
<div id="quick_nav_container">
 +
    <div class="quick_nav_box" id=nav_box1">
 +
        <h5><a href="https://2015.igem.org/Team:CU_Boulder/project/motivation">Problem</a></h5>
 +
    </div>
 +
    <div class="quick_nav_box" id=nav_box2">
 +
        <h5><a href="https://2015.igem.org/Team:CU_Boulder/Description">Solution</a></h5>
 +
    </div>
 +
    <div class="quick_nav_box" id=nav_box3">
 +
        <h5><a href="https://2015.igem.org/Team:CU_Boulder/project/results">Results</a></h5>
 +
    </div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<p></p>
 +
<!--<h1>Abstract</h1>-->
 +
        <!--<p class="box-float-right">I am a sample box. I hold content and look, I have a shadow. I am also personified for some reason. I am not as awesome as the Fact Sphere.</p>-->
 
   
 
   
  
−
<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
+
 
−
</article>
+
<div class="margins"> 
 +
<div id="abstract">
 +
<h1>Abstract</h1>
 +
 
 +
<p>Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds. In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water. To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking.  Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase.  Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.</p>
 +
 
 +
</div>
 +
</div>
  
  
  
 +
</div>
 +
</body>
  
 
</html>
 
</html>
 
{{CU_Boulder_footer}}
 
{{CU_Boulder_footer}}

Latest revision as of 04:48, 18 September 2015

File:Example.jpg


<!DOCTYPE html> Team:CU_Boulder - 2015.igem.org

UNIVERSITY OF COLORADO

NAPHTHALENE RECORDERS




A Big Fracking Deal

Abstract

Fracking is a common method for extracting natural gas and other fossil fuels from the ground, but it requires the use of many hazardous and carcinogenic compounds. In Colorado, ground water contamination from fracking has endangered many communities’ safe drinking water. To address this environmental health issue, we developed a biosensor to detect naphthalene, a common reagent in fracking. Naphthalene detection and output signal amplification can be achieved using the Lux promoter and the Bxb1 integrase. Our biosensor uses a naphthalene induced promoter located upstream of the BxB1 integrase. Once expressed, Bxb1 acts on a logic gate to express RFP. A Lux cell-cell signaling system from V. fischeri will enable our system to be more sensitive at low concentrations. Our biosensor could be housed within a stake-shaped device, containing a pump to obtain ground water and a live culture box kept at homeostatic conditions.

Team:CU-Boulder - 2015.igem.org