Difference between revisions of "Team:SCU China/test"

 
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</head><body><div id=start><div id=bg class=grey></div><button id=lm class="button button-large button-rounded button-longshadow-left button-highlight">Learn More</button></div>
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                         <ul class="menu">
 
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        <li role="presentation"><a href=https://2015.igem.org/Team:SCU_China/Home>Home</a></li>
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<li class="nav-current" role="presentation"><a href=/>Home</a></li>
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<li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Pangu>Pangu</a></li>
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<li role="presentation" ><a href=/pangu.html>Pangu</a></li>
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<li role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Project <span class=caret></span></a><ul class=dropdown-menu><li><a href=#>Overview</a></li><li><a href=#>Carbon Fixation</a></li><li><a href=#>Nitrogen Fixation</a></li><li><a href=#>Safety Project</a></li><li><a href=#>Modelling</a></li><li><a href=#>Results</a></li><li><a href=#>Software</a></li><li><a href=#>Notebook</a></li></ul></li><li role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Human Practice <span class=caret></span></a><ul class=dropdown-menu><li><a href=dist/1.html>ACMA</a></li><li><a href=#>Collaborating</a></li><li><a href=#>Meet Up</a></li></ul></li><li role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Team <span class=caret></span></a><ul class=dropdown-menu><li><a href=#>Members</a></li><li><a href=#>Attribution</a></li></ul></li><li role="presentation"><a href=#>Entrepreneurship</a></li>
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<li role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Team <span class=caret></span></a>
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<ul class=dropdown-menu>
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<li><a href=https://2015.igem.org/Team:SCU_China/Atributions>Atributions</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/Member>Member</a></li>
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<li role="Project" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Project<span class=caret></span></a>
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<li><a href=https://2015.igem.org/Team:SCU_China/Description>Description</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/CarbonFixation>Carbon Fixation</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/NitrogenFixation>Nitrogen Fixation</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/Test>Test</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/prom>Promoter</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/Modeling>Modeling</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/Notebook>Notebook</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/Safe>Safe</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/Design>Design</a></li>
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<li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Parts>Parts</a></li>
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<li role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Tool and Method<span class=caret></span></a>
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<li><a href=https://2015.igem.org/Team:SCU_China/Protocol>Protocol</a></li>
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<li><a href=https://2015.igem.org/Team:SCU_China/Software>Software</a></li>
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<li class="nav-current" role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Activities<span class=caret></span></a>
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<li><a href=https://2015.igem.org/Team:SCU_China/Practices>Human Practice</a></li>
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<li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Achievement>Achievement</a></li>
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         <h1 class="post-title">关于 Ghost 中文网</h1>
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         <h1 class="post-title">Colleberation</h1>
 
     </header>
 
     </header>
  
 
     <section class="post-content">
 
     <section class="post-content">
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        <blockquote>
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  <p>Ghost 是基于 Node.js 的开源博客平台，由前 WordPress UI 部门主管 <a href="http://john.onolan.org/">John O’Nolan</a> 和 WordPress 高级工程师（女） <a href="http://hannah.wf/">Hannah Wolfe</a> 创立，目的是为了给用户提供一种更加纯粹的内容写作与发布平台。</p>
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<p><img src="http://static.ghostchina.com/image/b/5e/877fa798be75dbe791dba07ac4811.jpg" alt="John O’Nolan 和 Hannah Wolfe" /></p>
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<div class="container ">
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<div class="col-lg-7 well">
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<div class="col-lg-3">
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<img src="https://static.igem.org/mediawiki/2014/c/c8/Scu-znh.jpg" style="max-width: 100%;">
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<h3>Nianhui Zhang</h3>
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<p>It is my pleasure to see the growth of the students full of energy and vitality.</p>
 +
</div></div></div>
  
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<p>2013 年 9 月份，Ghost 正式向公众发布，Ghost 中文网也随之上线。 <br />
 
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Ghost 中文网的目标是致力于 Ghost 开源博客系统在国内的推广，与广大 Ghost 用户分享 Ghost 相关的知识、技巧。</p>
 
  
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<hr />
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</section>
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</article>
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</main>
  
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<p>如果你也喜欢 Ghost ，可以通过以下方式与我们交流：</p>
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<aside class="col-md-4 sidebar ">
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<div class="widget hidden-xs hidden-sm " data-spy="affix" data-offset-top="60" data-offset-bottom="200">
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<h4 class="title">Navigation</h4>
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<ul id="nav" class="nav tag-cloud">
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<li><a href="#main-menu">Top</a></li>
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<li><a href="#1">University of Electronic Science and Technology of China</a></li>
 +
<li><a href="#2">Xi'an Jiaotong-Liverpool University </a></li>
  
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<ul>
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<li>微博：<a href="http://weibo.com/ghostchinacom">@ghostchinacom</a></li>
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                            </ul>
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<li>QQ群：309172035</li>
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</div>
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</ul>
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            </aside>
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<p>合作站点：</p>
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<ul>
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<li><a href="http://www.bootcss.com/">Bootstrap 中文网</a></li>
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<li><a href="http://www.bootcdn.cn/">开放 CDN 服务</a></li>
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<li><a href="http://www.gruntjs.net/">Grunt 中文网</a></li>
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<li><a href="http://www.golaravel.com/">Laravel 中文网</a></li>
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<li><a href="http://www.jquery123.com/">jQuery 中文文档</a></li>
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<p>合作伙伴：</p>
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<li><a href="http://www.aliyun.com/">阿里云</a></li>
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<p>赞助商：</p>
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<ul>
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<li><a href="https://www.upyun.com/">又拍云</a></li>
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<li><a href="http://www.ucloud.cn/">UCloud</a></li>
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<li><a href="http://www.qiniu.com/">七牛云存储</a></li>
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    </section>
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<div style="backgroundcolor:#edece4"><div class=container>
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<h1>E. pangu: The Pioneer of Mars</h1>
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<h2><strong>Background</strong></h2>
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<p>Since the onset of industrial era, the environment on earth keeps getting worse day by day because of the burning of fossil fuel and continuous deforestation. Recently, the discovery of Kepler-452b publicly announced by NASA calls public attention to space immigration again. As a matter of fact, the immigration to Mars, the most earthlike planet in solar system, still should be considered as priority. </p>
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<p>Before Mars immigration for human beings, it is necessary to send pioneer organism there to assess the feasibility of human immigration. What’s more, special pioneer organism can be also used to change the Martian atmosphere to make it more suitable for other organisms. The first three gaseous components of Martian atmosphere are carbon dioxide (96%), argon (1.93%) and  nitrogen (1.89%)[1]. Among these components, CO2 and N2 are ubiquitous on earth. Thus, SCU_iGEM team aim to create a pioneer organism which is able to carry out carbon fixation and nitrogen fixation simultaneously. After inorganic carbon and nitrogen are fixed into organic substances, they could act as nutrients server for other immigrant organisms in the future.</p>
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<h2><strong>Project overview</strong></h2>
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<p>In this summer, we are going to design a system which is able to fix inorganic carbon and nitrogen simultaneously. This system consists of 2 types of E.coli: nitrogen fixation E.coli and carbon fixation E.coli. We hope this system will play the role of pioneer organism on Mars immigration. Based on this purpose, we call them E. pangu collectively, which is named after the creator of universe, Pangu in Chinese mythology, as they will reclaim Mars for future immigrated organisms. </p>
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<h3><strong>Our project contains the following contents:</strong></h3>
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<h3><strong>1.Nitrogen fixation E.pangu</strong><strong></strong></h3>
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<p>Azotobacters are nitrogen fixation bacteria. They catalyze N2 reduction into ammonia by nitrogenase. The nitrogen fixation genome contains a large number of modules, or clusters. After deleting unnecessary modules[2], we will transform the minimal nitrogen fixation genome from azotobacter Paenibacillus sp. WLY78 into E.coli to construct nitrogen fixation E.pangu.</p>
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<h3><strong>2.Carbon fixation E.pangu</strong></h3>
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<p>Many anaerobes carry out carbon fixation by Wood-Ljungdahl pathway. One of the core enzymes in this pathway is acetyl-CoA synthetase/carbon monoxide dehydrogenase (ACS/CODH), a bifunctional enzyme which can reduce CO2 into acetyl-CoA, the central molecule in metabolism. We will transform the gene of ACS/CODH into E.coli to construct our carbon fixation E.pangu.</p>
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<h3><strong>3.Testing</strong></h3>
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<p>In this part, we will introduce gene editing technique called CRISPR-Cas9 to knock off the gene of pyruvate dehydrogenase(PDH) complex of carbon fixation E.pangu. Without PDH, the normal pathway of acetyl-CoA producing is cut off. Thus, if our carbon fixation E.pangu was successfully constructed, they can grow normally without PDH. </p>
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<h2><strong>Reference</strong></h2>
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<h4>[1]Krasnopolsky, V A.; Feldman, P D. Detection of Molecular Hydrogen in the Atmosphere of Mars. Science, 2001, 294:1914-1917.</h4>
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<h4>[2]Wang L, Liu Z, Zhao D, Liu X, et al. A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli. PLoS Genet, 2013, 9:e1003865.</h4>
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<h4>[3]Lindahl P A. The Ni-containing carbon monoxide dehydrogenase family: Light at the end of the tunnel. Biochemistry, 2002, 41:2097-2105.</h4>
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Latest revision as of 06:48, 18 September 2015

Colleberation

Nianhui Zhang

It is my pleasure to see the growth of the students full of energy and vitality.