@@ Line 1: / Line 1: @@
-<html lang="en" dir="ltr" class="client-nojs">
+<html><style>
-<head>
-<meta charset="UTF-8" />
-<title>Team:SCU China/test - 2015.igem.org</title>
-<meta name="generator" content="MediaWiki 1.24.1" />
-<link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:SCU_China/test&amp;action=edit" />
-<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:SCU_China/test&amp;action=edit" />
-<link rel="shortcut icon" href="/favicon.ico" />
-<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2015.igem.org (en)" />
-<link rel="EditURI" type="application/rsd+xml" href="https://2015.igem.org/wiki/api.php?action=rsd" />
-<link rel="alternate" hreflang="x-default" href="/Team:SCU_China/test" />
-<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
-<link rel="alternate" type="application/atom+xml" title="2015.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" />
-<link rel="stylesheet" href="https://2015.igem.org/wiki/load.php?debug=false&amp;lang=en&amp;modules=mediawiki.legacy.commonPrint%2Cshared%7Cmediawiki.skinning.content.externallinks%7Cmediawiki.skinning.interface%7Cmediawiki.ui.button%7Cskins.igem.styles&amp;only=styles&amp;skin=igem&amp;*" />
-<!--[if IE 6]><link rel="stylesheet" href="/wiki/skins/Igem/IE60Fixes.css?303" media="screen" /><![endif]-->
-<!--[if IE 7]><link rel="stylesheet" href="/wiki/skins/Igem/IE70Fixes.css?303" media="screen" /><![endif]--><meta name="ResourceLoaderDynamicStyles" content="" />
-<style>a:lang(ar),a:lang(kk-arab),a:lang(mzn),a:lang(ps),a:lang(ur){text-decoration:none}
-/* cache key: 2015_igem_org:resourceloader:filter:minify-css:7:875d2068ffef0b9ce1dbab8400ce2379 */</style>
-<script src="https://2015.igem.org/wiki/load.php?debug=false&amp;lang=en&amp;modules=startup&amp;only=scripts&amp;skin=igem&amp;*"></script>
-<script>if(window.mw){
-mw.config.set({"wgCanonicalNamespace":"","wgCanonicalSpecialPageName":false,"wgNamespaceNumber":0,"wgPageName":"Team:SCU_China/test","wgTitle":"Team:SCU China/test","wgCurRevisionId":285384,"wgRevisionId":285384,"wgArticleId":33527,"wgIsArticle":true,"wgIsRedirect":false,"wgAction":"view","wgUserName":"Lucifer","wgUserGroups":["*","user","autoconfirmed"],"wgCategories":[],"wgBreakFrames":false,"wgPageContentLanguage":"en","wgPageContentModel":"wikitext","wgSeparatorTransformTable":["",""],"wgDigitTransformTable":["",""],"wgDefaultDateFormat":"dmy","wgMonthNames":["","January","February","March","April","May","June","July","August","September","October","November","December"],"wgMonthNamesShort":["","Jan","Feb","Mar","Apr","May","Jun","Jul","Aug","Sep","Oct","Nov","Dec"],"wgRelevantPageName":"Team:SCU_China/test","wgUserId":887,"wgUserEditCount":11,"wgUserRegistration":1428136187000,"wgUserNewMsgRevisionId":null,"wgIsProbablyEditable":true,"wgRestrictionEdit":[],"wgRestrictionMove":[],"wgWikiEditorEnabledModules":{"toolbar":false,"dialogs":false,"hidesig":true,"preview":false,"previewDialog":false,"publish":false}});
-}</script><script>if(window.mw){
-mw.loader.implement("user.options",function($,jQuery){mw.user.options.set({"ccmeonemails":0,"cols":80,"date":"default","diffonly":0,"disablemail":0,"editfont":"default","editondblclick":0,"editsectiononrightclick":0,"enotifminoredits":0,"enotifrevealaddr":0,"enotifusertalkpages":1,"enotifwatchlistpages":1,"extendwatchlist":0,"fancysig":0,"forceeditsummary":0,"gender":"unknown","hideminor":0,"hidepatrolled":0,"imagesize":2,"math":1,"minordefault":0,"newpageshidepatrolled":0,"nickname":"","norollbackdiff":0,"numberheadings":0,"previewonfirst":0,"previewontop":1,"rcdays":7,"rclimit":50,"rows":25,"showhiddencats":0,"shownumberswatching":1,"showtoolbar":1,"skin":"igem","stubthreshold":0,"thumbsize":5,"underline":2,"uselivepreview":0,"usenewrc":1,"watchcreations":1,"watchdefault":1,"watchdeletion":0,"watchlistdays":3,"watchlisthideanons":0,"watchlisthidebots":0,"watchlisthideliu":0,"watchlisthideminor":0,"watchlisthideown":0,"watchlisthidepatrolled":0,"watchmoves":0,"watchrollback":0,
-"wllimit":250,"useeditwarning":1,"prefershttps":1,"language":"en","variant-gan":"gan","variant-iu":"iu","variant-kk":"kk","variant-ku":"ku","variant-shi":"shi","variant-sr":"sr","variant-tg":"tg","variant-uz":"uz","variant-zh":"zh","searchNs0":true,"searchNs1":false,"searchNs2":false,"searchNs3":false,"searchNs4":false,"searchNs5":false,"searchNs6":false,"searchNs7":false,"searchNs8":false,"searchNs9":false,"searchNs10":false,"searchNs11":false,"searchNs12":false,"searchNs13":false,"searchNs14":false,"searchNs15":false});},{},{});mw.loader.implement("user.tokens",function($,jQuery){mw.user.tokens.set({"editToken":"3980b8c162f5455bdc642d8f17da8579+\\","patrolToken":"ed01f1b257bca2245c13a241d4b167b4+\\","watchToken":"ab83b2a27079d3198672f7757c6bd4bd+\\"});},{},{});
-/* cache key: 2015_igem_org:resourceloader:filter:minify-js:7:2e71fe92cf450661358a8c29ac601135 */
-}</script>
-<script>if(window.mw){
-mw.loader.load(["mediawiki.page.startup","mediawiki.legacy.wikibits","mediawiki.legacy.ajax"]);
-}</script>
-</head>
-<body class="mediawiki ltr sitedir-ltr ns-0 ns-subject page-Team_SCU_China_test skin-igem action-view">
-        <script type='text/javascript'         src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
-        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
-        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
-    <div id='globalWrapper'>
-        <div id='top_menu_under' class='noprint'></div>
-        <div id='top_menu_14' class='noprint'>Loading menubar.....</div> <!-- Will be replaced with the jQuery.load -->
-		<script>jQuery('#top_menu_14').load('https://2015.igem.org/cgi/top_menu_14/menubar_reply.cgi',
-						    {   t:"Team%3ASCU%20China%2Ftest",
-							a:"View+%2FTeam%3ASCU_China%2Ftest++Edit+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3ASCU_China%2Ftest%26action%3Dedit++History+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3ASCU_China%2Ftest%26action%3Dhistory++Move+%2FSpecial%3AMovePage%2FTeam%3ASCU_China%2Ftest++Unwatch+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3ASCU_China%2Ftest%26action%3Dunwatch%26token%3D7b510fd221dde9e1ce28efc72e7b96a5%252B%255C++Page+%2FTeam%3ASCU_China%2Ftest++Discussion+%2Fwiki%2Findex.php%3Ftitle%3DTalk%3ATeam%3ASCU_China%2Ftest%26action%3Dedit%26redlink%3D1++" });
-		</script>
-		<div id="content" class="mw-body" role="main">
-		    <a id="top"></a>
-		    <h1 id="firstHeading" class="firstHeading">
-		        <span dir="auto">Team:SCU China/test</span>
-		    </h1>
-		    <div id="bodyContent">
-			<div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><p><style>
 .firstHeading {display: none;} /*-- hides default wiki settings --*/
@@ Line 72: / Line 22: @@
 }
+    .nav1 {
+        background: #4baac8;
+    }
+    .nav1 a {
+        color: black;
+        font-style: italic;
+    }
+    .nav1 li a:hover,
+    .nav1 li a:focus {
+        background: #FF9900;
+    }
+    .nav1 .active {
+        font-weight: bold;
+        background: #CC6633;
+    }
+    .nav1 .nav1 {
+        display: none;
+    }
+    .nav1 .active .nav1 {
+        display: block;
+    }
+    .na1v .nav1 a {
+        font-weight: normal;
+        font-size: .85em;
+    }
+    .nav1 .nav1 span {
+        margin: 0 5px 0 2px;
+    }
+    .nav1 .nav1 .active a,
+    .nav1 .nav1 .active:hover a,
+    .nav1 .nav1 .active:focus a {
+        font-weight: bold;
+        padding-left: 30px;
+        border-left: 5px solid black;
+    }
+    .nav1 .nav1 .active span,
+    .nav1 .nav1 .active:hover span,
+    .nav1 .nav1 .active:focus span {
+        display: none;
+    }
 </style>
@@ Line 84: / Line 80: @@
+<link rel="stylesheet" href="https://2015.igem.org/Team:SCU_China/magnific-popup.min.css ?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css"/>
@@ Line 89: / Line 86: @@
-</head><body style="#edece4">
+</head>
+<body >
+<div data-spy="scroll" data-target=".scrollspy">
 <div style="background-color:#e6cfa5; text-align:center; margin-left:auto; margin-right:auto;">
 <img src="https://static.igem.org/mediawiki/2015/4/4b/Scu_banner.jpeg" ></div>
@@ Line 112: / Line 112: @@
                      <div class="collapse navbar-collapse" id="main-menu">
                          <ul class="menu">
-         <li class="nav-current" role="presentation"><a href=https://2015.igem.org/Team:SCU_China/>Home</a></li>
+         <li  role="presentation"><a href=https://2015.igem.org/Team:SCU_China/Home>Home</a></li>
 <li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Pangu>Pangu</a></li>
@@ Line 129: / Line 129: @@
 <li><a href=https://2015.igem.org/Team:SCU_China/CarbonFixation>Carbon Fixation</a></li>
 <li><a href=https://2015.igem.org/Team:SCU_China/NitrogenFixation>Nitrogen Fixation</a></li>
-<li><a href=https://2015.igem.org/Team:SCU_China/1Test>Test</a></li>
+<li><a href=https://2015.igem.org/Team:SCU_China/Test>Test</a></li>
-<li><a href=https://2015.igem.org/Team:SCU_China/prom>prom</a></li>
+<li><a href=https://2015.igem.org/Team:SCU_China/prom>Promoter</a></li>
 <li><a href=https://2015.igem.org/Team:SCU_China/Modeling>Modeling</a></li>
 <li><a href=https://2015.igem.org/Team:SCU_China/Notebook>Notebook</a></li>
 <li><a href=https://2015.igem.org/Team:SCU_China/Safe>Safe</a></li>
+<li><a href=https://2015.igem.org/Team:SCU_China/Design>Design</a></li>
 </ul></li>
 <li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Parts>Parts</a></li>
-<li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Safety>Safety</a></li>
 <li role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Tool and Method<span class=caret></span></a>
@@ Line 148: / Line 148: @@
 </ul></li>
-<li role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Activities<span class=caret></span></a>
+<li class="nav-current" role="presentation" class=dropdown><a href=# class=dropdown-toggle data-toggle=dropdown role=button aria-haspopup=true aria-expanded=false>Activities<span class=caret></span></a>
 <ul class=dropdown-menu>
 <li><a href=https://2015.igem.org/Team:SCU_China/Practices>Human Practice</a></li>
@@ Line 156: / Line 156: @@
 <li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Achievement>Achievement</a></li>
-<li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Description>Improving</a></li>
-<li role="presentation" ><a href=https://2015.igem.org/Team:SCU_China/Design>Design</a></li>
@@ Line 173: / Line 173: @@
                  <main class="col-md-8 main-content">
-<div id="carousel-example-generic" class="carousel slide" data-ride="carousel">
-  <!-- Controls -->
-  <a class="left carousel-control" href="#carousel-example-generic" role="button" data-slide="prev">
-    <span class="glyphicon glyphicon-chevron-left" aria-hidden="true"></span>
-    <span class="sr-only">Previous</span>
-  </a>
-  <a class="right carousel-control" href="#carousel-example-generic" role="button" data-slide="next">
-    <span class="glyphicon glyphicon-chevron-right" aria-hidden="true"></span>
-    <span class="sr-only">Next</span>
-  </a>
-</div>
-</div>
 <article class="post page">
      <header class="post-head">
-         <h1 class="post-title">Crispr And Verification</h1>
+         <h1 class="post-title">Colleberation</h1>
      </header>
@@ Line 198: / Line 184: @@
+				<div class="container ">
+<div class="col-lg-7 well">
+				<div class="col-lg-3">
+<img src="https://static.igem.org/mediawiki/2014/c/c8/Scu-znh.jpg" style="max-width: 100%;">
+				</div>
+				<div class="col-lg-6">
+<h3>Nianhui Zhang</h3>
+				<p>It is my pleasure to see the growth of the students full of energy and vitality.</p>
+			</div></div></div>
-<audio src="https://static.igem.org/mediawiki/2015/c/c2/Scu-music1.ogg" controls="controls">
-Your browser does not support the audio tag.
-</audio>
-<h2 class="back"><strong>Crispr</strong></h2>
-<h3 class="back"><strong>Introduction</strong></h3>
-<p>The mean function of carbon fixation E.pangu is transforming inorganic substances into organic substances. Unfortunately, the process will definitely be affected by their natural ability of utilizing glucose to serve energy to E.pangu themselves. Thus, our project includes a part in which we will knockout the genes of glycolysis to verify the functions of carbon fixation E.pangu. The gene circuit for this part is shown in figure 1:</p>
-<img src="https://static.igem.org/mediawiki/2015/b/ba/7BT-135SP0BQF%60-A8-~VY-5.png" title = "Fig. 1. Gene circuit of carbon fixation testing" />
-<h6 class="back"><strong>Fig. 1. Gene circuit of carbon fixation testing</strong></h6>
-<p>Here we introduced a gene editing technique called CRISPR Cas9 to knock out glycolysis of E.pangu. CRISPR is widely used to edit genome based on the complexity of sgRNA and Cas9 protein. Unlike other gene edition technique, CRISPR can be designed in one dimension instead of stereoscopy. Thus, when targeting different sites, we just need to design corresponding sequences. After knocking out several key enzymes, we will be able to regulate the metabolism of E.pangu, so that the ability of carbon fixation of our engineered microbe can be qualified.</p>
-<p>At the initial stage of bacterial growth, the energy requirement is relatively high. To ensure the normal growth at this stage, we added a regulated promoter before Cas9 protein, through which the glucose catabolism of our bacteria can be regulated. That is, we will not knock out the glycolysis system until the cell growth has reached stationary phase.</p>
-<hr style="border-bottom: 1px solid #DA6426;margin-bottom: 60px;margin-top: 60px; margin-left:-35px;margin-right: -35px;">
-<h3 class="back"><strong>Results</strong></h3>
-<h4 class="back"><strong> 1.pRhl Insight</strong></h4>
-<p>In this summer, we are going to design a system which is able to fix inorganic carbon and nitrogen simultaneously. This system consists of 2 types of E.coli: nitrogen fixation E.coli and carbon fixation E.coli. We hope this system will play the role of pioneer organism on Mars immigration. Based on this purpose, we call them E. pangu collectively, which is named after the creator of universe, Pangu in Chinese mythology, as they will reclaim Mars for future immigrated organisms. </p>
-<h3><strong>Our project contains the following contents:</strong></h3>
-<h3><strong>1.Nitrogen fixation E.pangu</strong><strong></strong></h3>
-<p>Azotobacters are nitrogen fixation bacteria. They catalyze N2 reduction into ammonia by nitrogenase. The nitrogen fixation genome contains a large number of modules, or clusters. After deleting unnecessary modules[2], we will transform the minimal nitrogen fixation genome from azotobacter Paenibacillus sp. WLY78 into E.coli to construct nitrogen fixation E.pangu.</p>
-<h3><strong>2.Carbon fixation E.pangu</strong></h3>
-<p>Many anaerobes carry out carbon fixation by Wood-Ljungdahl pathway. One of the core enzymes in this pathway is acetyl-CoA synthetase/carbon monoxide dehydrogenase (ACS/CODH), a bifunctional enzyme which can reduce CO2 into acetyl-CoA, the central molecule in metabolism. We will transform the gene of ACS/CODH into E.coli to construct our carbon fixation E.pangu.</p>
-<h3><strong>3.Testing</strong></h3>
-<p>In this part, we will introduce gene editing technique called CRISPR-Cas9 to knock off the gene of pyruvate dehydrogenase(PDH) complex of carbon fixation E.pangu. Without PDH, the normal pathway of acetyl-CoA producing is cut off. Thus, if our carbon fixation E.pangu was successfully constructed, they can grow normally without PDH. </p>
-<hr style="border-bottom: 1px solid #DA6426;margin-bottom: 60px;margin-top: 60px; margin-left:-35px;margin-right: -35px;">
-<h2><strong>Reference</strong></h2>
-<h4>[1]Krasnopolsky, V A.; Feldman, P D. Detection of Molecular Hydrogen in the Atmosphere of Mars. Science, 2001, 294:1914-1917.</h4>
-<h4>[2]Wang L, Liu Z, Zhao D, Liu X, et al. A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli. PLoS Genet, 2013, 9:e1003865.</h4>
-<h4>[3]Lindahl P A. The Ni-containing carbon monoxide dehydrogenase family: Light at the end of the tunnel. Biochemistry, 2002, 41:2097-2105.</h4>
   </section>
 </article>
 </main>
-<aside class="col-md-4 sidebar scrollspy">
+<aside class="col-md-4 sidebar ">
-<div class="widget" data-spy="affix" data-offset-top="60" data-offset-bottom="200">
+<div class="widget hidden-xs hidden-sm " data-spy="affix" data-offset-top="60" data-offset-bottom="200">
-	<h4 class="title">社区</h4>
+	<h4 class="title">Navigation</h4>
-	<div class="content community">
+	<ul id="nav" class="nav tag-cloud">
-		<p>QQ群：277327792</p>
-		<p><a href="http://wenda.ghostchina.com/" title="Ghost中文网问答社区" target="_blank" onclick="_hmt.push(['_trackEvent', 'big-button', 'click', '问答社区'])"><i class="fa fa-comments"></i> 问答社区</a></p>
+<li><a href="#main-menu">Top</a></li>
-		<p><a href="http://weibo.com/ghostchinacom" title="Ghost中文网官方微博" target="_blank" onclick="_hmt.push(['_trackEvent', 'big-button', 'click', '官方微博'])"><i class="fa fa-weibo"></i> 官方微博</a></p>
+<li><a href="#1">University of Electronic Science and Technology of China</a></li>
-	</div>
+<li><a href="#2">Xi'an Jiaotong-Liverpool University </a></li>
+                            </ul>
 </div>
               </aside>
@@ Line 256: / Line 219: @@
 </div>
 </section>
+<script src="https://2015.igem.org/Team:SCU_China/magnific-popupjs?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
+<script>
+$('.image-popup-no-margins').magnificPopup({
+		type: 'image',
+		closeOnContentClick: true,
+		closeBtnInside: false,
+		fixedContentPos: true,
+		mainClass: 'mfp-no-margins mfp-with-zoom', // class to remove default margin from left and right side
+		image: {
+			verticalFit: true
+		},
+		zoom: {
+			enabled: true,
+			duration: 300 // don't foget to change the duration also in CSS
+		}
+	});
+</script>
 <script src="https://2015.igem.org/Team:SCU_China/jquerynav?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
 <script src="https://2015.igem.org/Team:SCU_China/bootstrap?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
@@ Line 262: / Line 241: @@
 <script src="https://2015.igem.org/Team:SCU_China/jQueryheadroom?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
 <script src="https://2015.igem.org/Team:SCU_China/homejs?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
+</div>
 </body>
-</p>
-<!--
-NewPP limit report
-CPU time usage: 0.004 seconds
-Real time usage: 0.004 seconds
-Preprocessor visited node count: 4/1000000
-Preprocessor generated node count: 24/1000000
-Post‐expand include size: 0/2097152 bytes
-Template argument size: 0/2097152 bytes
-Highest expansion depth: 2/40
-Expensive parser function count: 0/100
--->
-<!-- Saved in parser cache with key 2015_igem_org:pcache:idhash:33527-0!*!*!*!*!*!* and timestamp 20150917041946 and revision id 285384
- -->
-</div>			<div class="visualClear"></div>
-		    </div>
-		</div>
-    </div>
-	</body>
 </html>

Difference between revisions of "Team:SCU China/test"

Latest revision as of 06:48, 18 September 2015

Nianhui Zhang