Difference between revisions of "Team:CityU HK/Results"

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<div class="paragraph" style="text-align:left;"><font size="2"><strong style="color: rgb(42, 42, 42);"><span "mso-bidi-font-size:="" 13.5pt;font-family:&quot;arial&quot;,sans-serif;color:#333333;mso-fareast-language:zh-hk"="">Figure 2.</span></strong><strong style="color: rgb(42, 42, 42);"><span "mso-bidi-font-size:="" 12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;="" color:black;mso-font-kerning:0pt"="" style=""> Quantitative RT-PCR analysis of <em style="">lacZ</em> and <em style="">lacY</em> transcripts in E. coli <em style="">DH5&alpha;</em> host cells.</span></strong><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;="" mso-fareast-font-family:&quot;times="" roman&quot;;color:black;mso-font-kerning:0pt"=""><font color="#2a2a2a">&nbsp;</font></span><br /><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;="" mso-fareast-font-family:&quot;times="" roman&quot;;color:black;mso-font-kerning:0pt"=""><font size ="3"><font color="#2a2a2a">Expression of the </font><em style="color: rgb(42, 42, 42);">lacZ</em><font color="#2a2a2a"> and </font><em style="color: rgb(42, 42, 42);">lacY</em><font color="#2a2a2a"> genes was determined in control </font><em style="color: rgb(42, 42, 42);">DH5&alpha;</em><font color="#2a2a2a"> cells</font><font color="#24678d"> <strong>(</strong></font></span><strong><span "mso-bidi-font-size:12.0pt;font-family:="" &quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;color:#0f1368;="" mso-font-kerning:0pt"="" style=""><font color="#24678d">BLUE</font></span></strong><span "mso-bidi-font-size:="" 12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;="" color:black;mso-font-kerning:0pt"=""><strong><font color="#24678d">) </font></strong><font color="#2a2a2a">and BBa_S04055 recombinant </font><em style="color: rgb(42, 42, 42);">DH5&alpha;</em><font color="#2a2a2a"> cells</font><font color="#da4444"><strong> (</strong></font></span><span "mso-bidi-font-size:="" 12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;="" color:#ca2323;mso-font-kerning:0pt"=""><font color="#da4444"><strong>RED</strong></font></span><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:="" &quot;times="" roman&quot;;color:black;mso-font-kerning:0pt"=""><font color="#da4444"><strong>)</strong></font><font color="#2a2a2a">.</font></span><span "font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;color:#333333;="" mso-fareast-language:zh-hk"="" style="color: rgb(42, 42, 42);"> </span><span "mso-bidi-font-size:="" 12.0pt;font-family:&quot;arial&quot;,sans-serif;color:#333333;mso-fareast-language:zh-hk"="" style=""><font color="#2a2a2a">The cells were cultured overnight in LB medium and harvested for total RNA extraction.</font></span><u style=""><span "font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;="" color:#333333;mso-fareast-language:zh-hk"="" style=""></span></u><br /></font><span style=""></span><br /><span style=""></span></div>
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<div class="paragraph" style="text-align:left;"><font size="2"><strong style="color: rgb(42, 42, 42);"><span "mso-bidi-font-size:="" 13.5pt;font-family:&quot;arial&quot;,sans-serif;color:#333333;mso-fareast-language:zh-hk"="">Figure 1.</span></strong><strong style="color: rgb(42, 42, 42);"><span "mso-bidi-font-size:="" 12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;="" color:black;mso-font-kerning:0pt"="" style=""> Quantitative RT-PCR analysis of <em style="">lacY</em> and <em style="">lacZ</em> expression in <em style="">E. coli </em>cells.</span></strong><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;="" mso-fareast-font-family:&quot;times="" roman&quot;;color:black;mso-font-kerning:0pt"=""><font color="#2a2a2a">&nbsp;</font></span><br /><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;="" mso-fareast-font-family:&quot;times="" roman&quot;;color:black;mso-font-kerning:0pt"=""><font size ="3"><font color="#2a2a2a">Expression of the </font><em style="color: rgb(42, 42, 42);">lacZ</em><font color="#2a2a2a"> and </font><em style="color: rgb(42, 42, 42);">lacY</em><font color="#2a2a2a"> genes was measured in control DH5&alpha; cells </font><strong><span "mso-bidi-font-size:12.0pt;font-family:="" &quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;color:#0f1368;="" mso-font-kerning:0pt"="" style=""><font color="#24678d">(BLUE</font></span></strong><span "mso-bidi-font-size:="" 12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;="" color:black;mso-font-kerning:0pt"=""><strong><font color="#24678d">) </font></strong><font color="#2a2a2a">and BBa_S04055 recombinant </font><em style="color: rgb(42, 42, 42);">DH5&alpha;</em><font color="#2a2a2a"> cells</font><font color="#da4444"><strong> (</strong></font></span><span "mso-bidi-font-size:="" 12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:&quot;times="" roman&quot;;="" color:#ca2323;mso-font-kerning:0pt"=""><font color="#da4444"><strong>RED</strong></font></span><span "mso-bidi-font-size:12.0pt;font-family:&quot;arial&quot;,sans-serif;mso-fareast-font-family:="" &quot;times="" roman&quot;;color:black;mso-font-kerning:0pt"=""><font color="#da4444"><strong>)</strong></font><font color="#2a2a2a">.</font></span><span "font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;color:#333333;="" mso-fareast-language:zh-hk"="" style="color: rgb(42, 42, 42);"> </span><span "mso-bidi-font-size:="" 9.0pt;font-family:&quot;arial&quot;,sans-serif;color:#333333;mso-fareast-language:zh-hk"="" style=""><font color="#2a2a2a"><i>Cells were cultured overnight in LB medium + antibiotic and total RNA harvested for qRT-PCR using 16S rRNA for normalization.</i></font></span><u style=""><span "font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;="" color:#333333;mso-fareast-language:zh-hk"="" style=""></span></u><br /></font><span style=""></span><br /><span style=""></span></div>
  
  
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<h2 class="wsite-content-title" style="text-align:left;"><strong style=""><u style=""><span "font-size:18.0pt;="" font-family:&quot;arial&quot;,sans-serif;color:#333333"="" style=""><font size="6">Quantitative real-time PCR</font></span></u></strong><br /><span style=""></span></h2>
 
<h2 class="wsite-content-title" style="text-align:left;"><strong style=""><u style=""><span "font-size:18.0pt;="" font-family:&quot;arial&quot;,sans-serif;color:#333333"="" style=""><font size="6">Quantitative real-time PCR</font></span></u></strong><br /><span style=""></span></h2>
  
<div class="paragraph" style="text-align:justify;"><font size="3"><span "mso-bidi-font-size:13.5pt;="" font-family:&quot;arial&quot;,sans-serif;color:black;mso-themecolor:text1"="">Quantitative real-time PCR analyses showed that&nbsp;both </span><em><span "mso-bidi-font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;="" color:black;mso-themecolor:text1;mso-fareast-language:zh-hk"="" style="">lacY </span></em><span "mso-bidi-font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;="" color:black;mso-themecolor:text1;mso-fareast-language:zh-hk;mso-bidi-font-style:="" italic"="">and<em style=""> lacZ </em></span><span "mso-bidi-font-size:13.5pt;="" font-family:&quot;arial&quot;,sans-serif;color:black;mso-themecolor:text1"="">transcripts were expressed at high levels in the recombinant <em style="">E. coli</em> cells as compared to control (Figure 2). The <em style="">lacY</em> gene expression was 9-fold higher in recombinant cells than the control cells. For the <em style="">lacZ</em> gene</span><em><span "mso-bidi-font-size:13.5pt;="" font-family:&quot;arial&quot;,sans-serif;color:black;mso-themecolor:text1;mso-fareast-language:="" zh-hk"="" style=""> </span></em><span "mso-bidi-font-size:13.5pt;="" font-family:&quot;arial&quot;,sans-serif;color:black;mso-themecolor:text1;mso-fareast-language:="" zh-hk;mso-bidi-font-style:italic"="">mRNA expression, the recombinant cells </span><span "mso-bidi-font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;="" color:black;mso-themecolor:text1"="">were 2-fold higher than the control cells. </span><span "mso-bidi-font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;="" color:black;mso-themecolor:text1;mso-fareast-language:zh-hk"="" style=""></span><br /><span style=""></span><br /><span style=""></span>  <span "font-size:12.0pt;mso-bidi-font-size:13.5pt;font-family:="" &quot;arial&quot;,sans-serif;mso-fareast-font-family:&#26032;&#32048;&#26126;&#39636;;mso-fareast-theme-font:minor-fareast;="" color:#333333;mso-ansi-language:en-us;mso-fareast-language:zh-hk;mso-bidi-language:="" ar-sa"=""><br /> </span>  <span "mso-bidi-font-size:13.5pt;font-family:&quot;arial&quot;,sans-serif;color:#333333;="" mso-fareast-language:zh-hk"=""></span></font><br /><span style=""></span><br /><span style=""></span></div>
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<div class="paragraph" style="text-align:justify;"><font size="3"><span "mso-bidi-font-size:13.5pt;="" font-family:&quot;arial&quot;,sans-serif;color:black;mso-themecolor:text1"="">Quantitative real-time PCR analysis showed that both lacY and lacZ mRNA transcripts are expressed at high levels in recombinant E. coli cells (harboring the BBa_S04055 biobrick) as compared to control DH5α cells (Figure 1). Expression of lacY and lacZ is 9- fold and 2-fold higher, respectively, in recombinant cells with respect to the control cells.</span></font></div>
 
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Revision as of 07:08, 18 September 2015

Results - iGEM2015 wiki