Difference between revisions of "Team:Warwick/Collaborations"

 
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<h5 class="sidebartitle">NTNU Modelling Collaboration</h5>
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<p>_______________________________________________________________________________________________________________________________________</p><h5>NTNU Modelling Collaboration</h5>
 
<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/1/13/Warwickntunuloo.png" height="120px" width="120px" border="1px"></p>
 
<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/1/13/Warwickntunuloo.png" height="120px" width="120px" border="1px"></p>
 
After we came up with a model of DNA Origami arms and the sequences for them and how they would bond we wanted to model the probability of the <i>E.coli</i> arms forming fully and how this probability would change as length of the arms increased. We came up with a basic model but thought it would be beneficial if we sought outside help with the mathematics. <br>
 
After we came up with a model of DNA Origami arms and the sequences for them and how they would bond we wanted to model the probability of the <i>E.coli</i> arms forming fully and how this probability would change as length of the arms increased. We came up with a basic model but thought it would be beneficial if we sought outside help with the mathematics. <br>
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<h5 class="sidebartitle">Oxford Collaboration</h5>
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<p>_______________________________________________________________________________________________________________________________________</p><h5>Oxford Collaboration</h5>
 
<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/7/7c/Warwickocfordlogo.png" height="120px" width="120px" border="1px"></p>
 
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One part of our project was create a DNA origami glue using a biobrick part from the distributed kit, specifically part BBa_K314110. Once we had designed it, we decided to collaborate with Oxford's iGEM team to help get it made.  
 
One part of our project was create a DNA origami glue using a biobrick part from the distributed kit, specifically part BBa_K314110. Once we had designed it, we decided to collaborate with Oxford's iGEM team to help get it made.  
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<h5 class="sidebartitle">Glasgow Collaboration</h5>
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<p>_______________________________________________________________________________________________________________________________________</p><h5>Glasgow Collaboration</h5>
 
<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/a/ae/2015-Glasgow-sticker.png" height="120px" width="120px" border="50px"></p>
 
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We had one particular experiment which repeatedly caused an issue for us at the gel extraction stage. We found it tricky to cut out the DNA from the gel under a blue light, and felt poor cutting technique could be the root of our problem. At the iGEM 2015 London Meet up we met the team from Glasgow University. They had just presented an ethidium bromide free method of producing gels, which then did not need to be visualised using a blue light. Instead these gels could be seen by eye, which we saw as extremely helpful to solve our issue. Glasgow kindly packaged off and sent us some AzureA, with which we set out producing the gel. This blue AzureA gel proved very useful, and it was easier to visualise clear bands just by eye. The collaboration helped us to solve our wet lab issues and supported the characterisation of Glasgow's gel staining method.
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We had one particular experiment which repeatedly caused an issue for us at the gel extraction stage. We found it difficult to get consistent results when using our gel extraction protocol, and thought it could be due to issues with the ethidium bromide gel we were using. At the iGEM 2015 London Meet up we met the team from Glasgow University. They had just presented an ethidium bromide free method of producing gels, which then did not need to be visualised using a blue light. Instead these gels were visible under normal conditions, which we saw as extremely helpful to solve our issue. Glasgow kindly packaged off and sent us some AzureA, with which we set out producing the gel. While this did not ultimately solve our issue (we ended up using a different dye altogether) we are still thankful for the opportunity to test out a fellow iGEM team's ideas.
 
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<h5 class="sidebartitle">Manchester-Graz Collaboration</h5></div>
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<p>_______________________________________________________________________________________________________________________________________</p> <h5>Manchester-Graz Collaboration</h5></div>
 
<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/8/80/Manchester-Graz_Banner_big_obelix.jpg" height="120px" width="240px" border="1px"></p>
 
<p><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/8/80/Manchester-Graz_Banner_big_obelix.jpg" height="120px" width="240px" border="1px"></p>
 
<p><p style="float: right;"><img src="https://static.igem.org/mediawiki/2015/8/86/Warwickmanceshter.jpg" height="480px" width="240px" border="10px"></p> <br><br><br><br>
 
<p><p style="float: right;"><img src="https://static.igem.org/mediawiki/2015/8/86/Warwickmanceshter.jpg" height="480px" width="240px" border="10px"></p> <br><br><br><br>

Latest revision as of 08:55, 18 September 2015

Warwick iGEM 2015