Difference between revisions of "Team:Paris Saclay/Notebook/July/24"
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| + | <html><p><i>Quantification, from left to right: 1. DNA Ladder, 2. BBa_K1707003#4, 3. BBa_K1399005, 4. BBa_B0015, 5. BBa_J23101, 6. Empty, 7. Empty</i></p></html> | ||
===Transformation=== | ===Transformation=== | ||
''by Seong-Koo'' | ''by Seong-Koo'' | ||
Revision as of 10:03, 18 September 2015
Contents
Friday 24th July
Lab Work
Digestion
by Pauline
Biobricks:
- BBa_K1707003 #4
- BBa_K1399005
- BBa_B0015
- BBa_J23101
In each tube:
- 10 µL plasmid
- 1µL of each enzyme
- 2 µL Buffer FastDigest
- 6µL H2O
Digestions:
- BBa_K1707003: XbaI + PstI
- BBa_K1399005: EcoRI + SpeI
- BBa_B0015: EcoRI + XbaI
- BBa_J23101: SpeI + PstI
Purification
by Pauline
Biobricks:
- BBa_K1707003
- BBa_K1399005
Agarose gel 1%, 110V
Verification of purification, from left to right: 1. DNA Ladder, 2. BBa_K1707003#4, 3. BBa_K1399005
We cut bands with a scalpelPurification
by Audrey
Biobricks:
- BBa_K1707003
- BBa_K1399005
- BBa_B0015
- BBa_J23101
With PCR Clean-up/Gel extraction kit from Macherey-Nigel
Quantification
Biobricks:
- BBa_K1707003
- BBa_K1399005
- BBa_B0015
- BBa_J23101
Agarose gel 1% Migration 110V
Quantification, from left to right: 1. DNA Ladder, 2. BBa_K1707003#4, 3. BBa_K1399005, 4. BBa_B0015, 5. BBa_J23101, 6. Empty, 7. Empty
Transformation
by Seong-Koo
Biobricks:
- BBa_K1707005
- BBa_K1707006
- BBa_K1707007
Soil experiment
by Seong Koo
Plates observation and count of CFU:
- Strain 1696:
- Dilution 1: 424 CFU
- Dilution 0.1: 75 CFU
- Dilution 0.01: 1 CFU
- Strain 1693:
- Dilution 1: 524 CFU
- Dilution 0.1: 35 CFU
- Dilution 0.01: 0 CFU
- Strain 1320:
- Dilution 1: 111 CFU
- Dilution 0.1: 13 CFU
- Dilution 0.01: 0 CFU
We take 1g of contaminated soil and treat it like yesterday to spread it on specific plates Incubation ON, 37°C
Interlab study: Cytometer
by Johan
https://2015.igem.org/Team:Paris_Saclay/Measurement#24th_July
Member present:
- Instructors: Alice
- Students: Coralie, Audrey, Pauline, Johan and Seong-Koo
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.