Difference between revisions of "Team:Warwick/Modeling"
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Modelling is a key part of synthetic biology. Some experiments take too long, are far too expensive, or the information required just can’t be found via lab work. This is where modelling comes in. We take information from the biologist, construct a theoretical framework, and then feed back to people in the lab about what they should do. | Modelling is a key part of synthetic biology. Some experiments take too long, are far too expensive, or the information required just can’t be found via lab work. This is where modelling comes in. We take information from the biologist, construct a theoretical framework, and then feed back to people in the lab about what they should do. | ||
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<p><a href="BindingAffinity"><h5>Binding Affinity Modelling</h5><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/6/66/Warwickmodeling5.png" align="right" height="100px" width="100px" border="1px"></p></p> </a> | <p><a href="BindingAffinity"><h5>Binding Affinity Modelling</h5><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/6/66/Warwickmodeling5.png" align="right" height="100px" width="100px" border="1px"></p></p> </a> | ||
| − | A big problem biologists encounter is the uncertainty of | + | A big problem biologists encounter is the uncertainty of binding especially in our design where zinc fingers bind to their sites. Therefore it is important to come up with a model which can calculate the number of cell and zinc finger binding sites required for a given output. This page discusses this and shows a program designed to dictate concentrations for the biologists to use. |
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<p><a href="Modelling4"><h5>DNA Beading Model</h5><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/a/a0/WarwickBead_Drawing.png" align="right" height="100px" width="100px" border="1px"></p></p> </a> | <p><a href="Modelling4"><h5>DNA Beading Model</h5><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/a/a0/WarwickBead_Drawing.png" align="right" height="100px" width="100px" border="1px"></p></p> </a> | ||
| − | Once we had a method of calculating the concentration of cells needed we had to model the number of cells required to make a certain shape. We also needed to invent a novel approach to creating 2D shapes using cells, this page discusses | + | Once we had a method of calculating the concentration of cells needed we had to model the number of cells required to make a certain shape. We also needed to invent a novel approach to creating 2D shapes using cells, this page discusses binding them to a longer string of DNA to form a pattern. |
<p><img src="https://static.igem.org/mediawiki/2015/2/29/Warwickbubbles2.png" height="120px" width="800px" border="1px"></p> | <p><img src="https://static.igem.org/mediawiki/2015/2/29/Warwickbubbles2.png" height="120px" width="800px" border="1px"></p> | ||
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<p><a href="modelling3"><h5>Tetrahedron Construction</h5><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/0/05/WarwickCaddy.png" align="right" height="100px" width="100px" border="1px"></p></p> </a> | <p><a href="modelling3"><h5>Tetrahedron Construction</h5><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/0/05/WarwickCaddy.png" align="right" height="100px" width="100px" border="1px"></p></p> </a> | ||
| − | The previous model of using DNA as a glue could create 3D shapes but would need vast amounts of unique zinc fingers. This wasn't possible with our time frame so we | + | The previous model of using DNA as a glue could create 3D shapes but would need vast amounts of unique zinc fingers. This wasn't possible with our time frame so we came up with a model which could create a 3D structure from the minimum amount of unique DNA using tetrahedrons as a base to build from. Cells would then be bound to the outside.<br> |
| − | <p><img src="https://static.igem.org/mediawiki/2015/ | + | <p><img src="https://static.igem.org/mediawiki/2015/b/be/Warwickbubbles5.png" height="120px" width="800px" border="1px"></p> |
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| + | <p><a href="Modelling1"><h5>NTNU Collaboration</h5><p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/1/13/Warwickntunuloo.png" align="right" height="100px" width="100px" border="1px"></p></p> | ||
| + | </a> | ||
| + | We dcided to get help for some of the modelling and NTNU were kind enough to oblige. This model deals with calculating bonding and binding affinities.<br><br> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2015/4/4f/Warwickbubbles9.png" height="120px" width="800px" border="1px"></p> | ||
Latest revision as of 10:10, 18 September 2015
