Difference between revisions of "Team:UChile-OpenBio/Description"
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<p>Our first system <span class="destacado">Lactadora</span></b> consists of E.coli lab strain with a synthetic gene circuit presented at the rigth. | <p>Our first system <span class="destacado">Lactadora</span></b> consists of E.coli lab strain with a synthetic gene circuit presented at the rigth. | ||
| − | We construct the ppH-TetR module to synthetize the TetR protein which is able to repress the pTetR promoter. This synthesis is up-regulated by a pH promoter which is induced when pH is lower than 5.5, otherwise (pH>5.5) there is no TetR protein production. In this last case, the second module (pTetR-DLDH-LuxI) will express the DLDH enzyme to synthetize lactate from pyruvate produced from a glucose molecule and diffuse to medium. | + | We construct the ppH-TetR module to synthetize the TetR protein which is able to repress the pTetR promoter. This synthesis is up-regulated by a pH promoter which is induced when pH is lower than 5.5, otherwise (pH>5.5) there is no TetR protein production. In this last case, the second module (pTetR-DLDH-LuxI) will express the DLDH enzyme to synthetize lactate from pyruvate produced from a glucose molecule and diffuse to medium.</p> |
| − | Also, will be expressed the LuxI protein to generate homoserine lactone (HSL), a quorum sensing molecule that can diffuse outside the cell. While concentration of lactate outside the cell increases, medium will turn it an acidic environment; the higher lactate concentration, the lower pH. | + | <p>Also, will be expressed the LuxI protein to generate homoserine lactone (HSL), a quorum sensing molecule that can diffuse outside the cell. While concentration of lactate outside the cell increases, medium will turn it an acidic environment; the higher lactate concentration, the lower pH. </p> |
| − | This way, when pH reaches a 5.5 value or lower, ppH-TetR module will be activated and TetR protein will repress the second module and synthesis of lactate will stop until pH increases again. </p> | + | <p>This way, when pH reaches a 5.5 value or lower, ppH-TetR module will be activated and TetR protein will repress the second module and synthesis of lactate will stop until pH increases again. </p> |
</div> | </div> | ||
<div class="half last"> | <div class="half last"> | ||
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| + | <img id="Esquema" class="middle top" alt="Bacteria SuperHeroe" src="https://static.igem.org/mediawiki/2015/3/31/UChile-Openbio-david_comentario_pag2.png" /> | ||
| + | <img id="Esquema" class="middle top" alt="Modules" src="https://static.igem.org/mediawiki/2015/c/c2/UChile-Openbio-DES_Lactadora_pag2.png" /> <br> | ||
| + | <h3>When pH<5.5, lactate production is OFF.</h3> | ||
| + | <img id="Esquema" class="middle top" alt="Modules" src="https://static.igem.org/mediawiki/2015/8/8d/UChile-Openbio-DES_Lactadora_activa_pag2.png" /><br> | ||
| + | <h3>When pH>5.5, lactate production is ON.</h3> | ||
</div> | </div> | ||
Revision as of 10:17, 18 September 2015
Titulo 1
Titulo 2
Titulo 3
Lactadora: Lactate production and pH regulation system
Develop a system that can produces lactate and regulates its concentration. Also, a homoserine lactone molecule would be produce at the same time to activate the second system (PLA production and exportation) by quorum sensing.
What parts did we use? And why?
What modules did we assembly?
How our Lactadora system does work?
Our first system Lactadora consists of E.coli lab strain with a synthetic gene circuit presented at the rigth. We construct the ppH-TetR module to synthetize the TetR protein which is able to repress the pTetR promoter. This synthesis is up-regulated by a pH promoter which is induced when pH is lower than 5.5, otherwise (pH>5.5) there is no TetR protein production. In this last case, the second module (pTetR-DLDH-LuxI) will express the DLDH enzyme to synthetize lactate from pyruvate produced from a glucose molecule and diffuse to medium.
Also, will be expressed the LuxI protein to generate homoserine lactone (HSL), a quorum sensing molecule that can diffuse outside the cell. While concentration of lactate outside the cell increases, medium will turn it an acidic environment; the higher lactate concentration, the lower pH.
This way, when pH reaches a 5.5 value or lower, ppH-TetR module will be activated and TetR protein will repress the second module and synthesis of lactate will stop until pH increases again.
When pH<5.5, lactate production is OFF.
