Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"
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<h1>BioBricks</h1> | <h1>BioBricks</h1> | ||
− | <h2>1. BBa_K1321000 | + | <h2>1. BBa_K1321000: STE2MAM2</h2> |
− | + | <p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.</p> | |
− | + | <h2>2. BBa_K1321001: pTEF1-pSUC2</h2> | |
− | <p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway. </p> | + | <p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low glucose concentrations.</p> |
− | <h2> | + | <h2>3. BBa_K1321002: pTPI1</h2> |
− | <p> | + | <p>promoter to TPI1. |
− | + | ||
</p> | </p> | ||
− | <h2>4. | + | <h2>4. BBa_K1321003: RecA</h2> |
− | <p> | + | <p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. |
− | <h2>5. | + | Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.</p> |
− | <p>From Deinococcus Radiodurans | + | <h2>5. BBa_K1321004: Ssb</h2> |
+ | <p>From Deinococcus Radiodurans. | ||
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair | Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair | ||
</p> | </p> |
Revision as of 11:01, 18 September 2015
Contents
BioBricks
1. BBa_K1321000: STE2MAM2
Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.
2. BBa_K1321001: pTEF1-pSUC2
The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low glucose concentrations.
3. BBa_K1321002: pTPI1
promoter to TPI1.
4. BBa_K1321003: RecA
Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.
5. BBa_K1321004: Ssb
From Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair