Difference between revisions of "Team:UiOslo Norway/Experiments/Restriction Digest"

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5 μl  Cutsmart</br>
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x μl DNA (1 μg total concentration)</br>
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1 μl Restriction Enzyme I</br>
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1 μl Restriction Enzyme II</br>
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up to 50 μl  MilliQ Water</br>
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</p>
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<hr style="height:2px;border:none;color:#472400;background-color:#333;" />
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<p>Total Reaction of 50 μl </p>
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<p>
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Incubate for 1 hour at 37 °C</br>
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</br>
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For restriction digests including NdeI, at first only 1 μl of NdeI was added and the digest incubated at 37 °C for 1 hour, afterwards NdeI was heat inactivated at 65 °C for 20 minutes and then the second restriction enzyme was added and the digest was again incubated for 1 hour.
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<!---------------sponsors------------------------->
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<center><h1>iGEM UiOslo 2015 is sponsored by:</h1>
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<hr style="height:2px;border:none;color:black;background-color:black;" />
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<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/e/e0/UiOslo_uio_logo.png" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/4/4a/UiOslo_IDT.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
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</center>
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<hr style="height:2px;border:none;color:black;background-color:black;" />
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<!--------end sponsors---------------------------->
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<!--------end sponsors---------------------------->
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</body>
 
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Latest revision as of 12:10, 18 September 2015

Restriction Digest:

Back to Protocols


5 μl Cutsmart
x μl DNA (1 μg total concentration)
1 μl Restriction Enzyme I
1 μl Restriction Enzyme II
up to 50 μl MilliQ Water

Total Reaction of 50 μl


Incubate for 1 hour at 37 °C

For restriction digests including NdeI, at first only 1 μl of NdeI was added and the digest incubated at 37 °C for 1 hour, afterwards NdeI was heat inactivated at 65 °C for 20 minutes and then the second restriction enzyme was added and the digest was again incubated for 1 hour.

iGEM UiOslo 2015 is sponsored by: