Latest revision as of 12:11, 18 September 2015

Shrimp Alkaline Phosphatase Treatment (Fermentas):

Following the provided protocol.

2 μl 10X Reaction Buffer
1 μl shrimp alkaline phosphatase
x μl DNA( 1 μg total concentration)
MilliQ up to 20 μl

Total Reaction of 20 μl

Incubate at 37 °C for 30 minutes
Heat inactivation at 65 °C for 15 minutes.

@@ Line 17: / Line 17: @@
 	protocol.
 	 </a>
+</br>
+</br>
 μl 10X Reaction Buffer</br>
 μl shrimp alkaline phosphatase</br>
@@ Line 32: / Line 33: @@
 </p>
-</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
+<div class="clear"></div>
+<!---------------sponsors------------------------->
+<center><h1>iGEM UiOslo 2015 is sponsored by:</h1>
+<hr style="height:2px;border:none;color:black;background-color:black;" />
+<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
+<img src="https://static.igem.org/mediawiki/2015/e/e0/UiOslo_uio_logo.png" Height="60px" hspace="15">
+<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
+<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
+<img src="https://static.igem.org/mediawiki/2015/4/4a/UiOslo_IDT.jpg" Height="60px" hspace="15">
+<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
+<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
+</center>
+<hr style="height:2px;border:none;color:black;background-color:black;" />
+<!--------end sponsors---------------------------->
+</body>
 </html>