Difference between revisions of "Team:UiOslo Norway/Experiments/Alkaline Phospatase"

(Created page with "{{UiOslo_Norway}} <html> <h1>Shrimp Alkaline Phosphatase Treatment (Fermentas):</h1> <a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments" > Back to Protocols </...")
 
 
(6 intermediate revisions by the same user not shown)
Line 10: Line 10:
 
Back to Protocols
 
Back to Protocols
 
</a>  
 
</a>  
 +
<p></br></p>
  
−
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
+
<p>
 +
 
 +
Following the provided <a href="http://www.biomart.cn/upload/asset/attachment/fermentas%E4%B8%AD%E5%9B%BD/coa_ef0511.pdf" >
 +
protocol.
 +
</a>
 +
</br>
 +
</br>
 +
2 μl 10X Reaction Buffer</br>
 +
1 μl shrimp alkaline phosphatase</br>
 +
x μl DNA( 1 μg total concentration)</br>
 +
MilliQ up to 20 μl</br>
 +
</p>
 +
<hr style="height:2px;border:none;color:#472400;background-color:#333;" />
 +
<p>Total Reaction of 20 μl</p>
 +
 
 +
</br></p>
 +
<p>
 +
Incubate at 37 °C for 30 minutes</br>
 +
Heat inactivation at 65 °C for 15 minutes.
 +
</p>
 +
 
 +
<div class="clear"></div>
 +
<!---------------sponsors------------------------->
 +
 
 +
 
 +
 
 +
<center><h1>iGEM UiOslo 2015 is sponsored by:</h1>
 +
<hr style="height:2px;border:none;color:black;background-color:black;" />
 +
<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/e/e0/UiOslo_uio_logo.png" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/4/4a/UiOslo_IDT.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
 +
</center>
 +
<hr style="height:2px;border:none;color:black;background-color:black;" />
 +
 
 +
<!--------end sponsors---------------------------->
 +
 
 +
</body>
 
</html>
 
</html>

Latest revision as of 12:11, 18 September 2015

Shrimp Alkaline Phosphatase Treatment (Fermentas):

Back to Protocols


Following the provided protocol.

2 μl 10X Reaction Buffer
1 μl shrimp alkaline phosphatase
x μl DNA( 1 μg total concentration)
MilliQ up to 20 μl

Total Reaction of 20 μl


Incubate at 37 °C for 30 minutes
Heat inactivation at 65 °C for 15 minutes.

iGEM UiOslo 2015 is sponsored by: