Difference between revisions of "Team:UiOslo Norway/Experiments/Alkaline Phospatase"

 
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Use Zero blunt TOPO PCR cloning Kit</br>
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Following the provided <a href="http://www.biomart.cn/upload/asset/attachment/fermentas%E4%B8%AD%E5%9B%BD/coa_ef0511.pdf" >
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protocol.
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</a>
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</br>
 
</br>
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2 μl 10X Reaction Buffer</br>
 
2 μl 10X Reaction Buffer</br>
 
1 μl shrimp alkaline phosphatase</br>
 
1 μl shrimp alkaline phosphatase</br>
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Incubate at 37 °C for 30 minutes
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Incubate at 37 °C for 30 minutes</br>
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Heatinactivation at 65 °C for 15 minutes.  
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Heat inactivation at 65 °C for 15 minutes.  
 
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<div class="clear"></div>
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<!---------------sponsors------------------------->
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<center><h1>iGEM UiOslo 2015 is sponsored by:</h1>
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<hr style="height:2px;border:none;color:black;background-color:black;" />
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<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/e/e0/UiOslo_uio_logo.png" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/4/4a/UiOslo_IDT.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
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<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
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</center>
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<hr style="height:2px;border:none;color:black;background-color:black;" />
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<!--------end sponsors---------------------------->
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</body>
 
</html>
 
</html>

Latest revision as of 12:11, 18 September 2015

Shrimp Alkaline Phosphatase Treatment (Fermentas):

Back to Protocols


Following the provided protocol.

2 μl 10X Reaction Buffer
1 μl shrimp alkaline phosphatase
x μl DNA( 1 μg total concentration)
MilliQ up to 20 μl

Total Reaction of 20 μl


Incubate at 37 °C for 30 minutes
Heat inactivation at 65 °C for 15 minutes.

iGEM UiOslo 2015 is sponsored by: