Difference between revisions of "Team:UiOslo Norway/Experiments/Transformation"

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Latest revision as of 12:23, 18 September 2015

Transformation of chemically competent E. coli cells

Back to Protocols


  • Add 1 µl of plasmid or 5 µl of Ligation reaction to chemically competent E. coli cells

  • Incubate on ice for 30 minutes

  • Heatshock: 42 °C for 45 seconds, 2 minutes on ice

  • Add 300 µl LB- Media and incubate at 37 °C shaking at 220 rpm for 1 hour

  • Plate on LB plates containing the appropriate antibiotic and incubate at 37 °C overnight.

    • iGEM UiOslo 2015 is sponsored by: