Difference between revisions of "Team:UiOslo Norway/Experiments/NMS medium"

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<p>
 
<p>
 
<b>10x NMS</b></br>
 
<b>10x NMS</b></br>
−
98.9 mM KNO3</br>
+
98.9 mM KNO<sub>3</sub></br>
−
43.8 mM MgSO4 * 6 H2O</br>
+
43.8 mM MgSO<sub>4</sub> * 6 H<sub>2</sub>O</br>
−
9.0 mM CaCl2</br>
+
9.0 mM CaCl<sub>2</sub></br>
−
up to 1L Water </br>
+
up to 1L Water </br></br>
 +
</br>
 +
<b>Ethylenediaminetetraacetic acid ferric sodium salt</b></br>
 +
103.5 mM FeEDTA</br>
 +
up to 100 ml Water</br></br>
 +
</br>
 +
<b>Sodium Molybdate dihydrate</b></br>
 +
1.07 mM NaMoO<sub>4</sub> *2H<sub>2</sub>O</br>
 +
up to 500 ml Water</br></br>
 +
</br>
 +
<b>Trace Elements solution</b></br>
 +
0.8 mM CuSO<sub>4</sub>*5 H<sub>2</sub>O</br>
 +
1.8 mM FeSO<sub>4</sub>*7H<sub>2</sub>O</br>
 +
1.4 mM ZnSO<sub>4</sub>*7H<sub>2</sub>O</br>
 +
0.24 mM H<sub>3</sub>BO<sub>3</sub></br>
 +
0.21 mM CoCl<sub>2</sub>*6H<sub>2</sub>O</br>
 +
0.74 mM EDTA-Na<sub>2</sub></br>
 +
0.1 mM MnCL<sub>2</sub>*4H<sub>2</sub>O</br>
 +
42.07 µM NiCl<sub>2</sub>*6H<sub>2</sub>O</br>
 +
up to 500 ml Water</br>
 +
</br>
 +
Store dark.</br>
 +
</br>
 +
<b>Phosphate buffer</b></br>
 +
198.25 mM Na<sub>2</sub>HPO<sub>4</sub>*12H<sub>2</sub>O</br>
 +
191.05 mM KH<sub>2</sub>PO<sub>4</sub></br>
 +
up to 100 ml Water</br>
 +
</br>
 +
Add H<sub>2</sub>O to 800 ml, adjust pH to 6,8 and add H<sub>2</sub>O to 1000 ml. Autoclave or sterile filter.</br>
 +
</br>
 +
<b>Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate</b></br>
 +
400.5 mM CuSO<sub>4</sub>*5H<sub>2</sub>O</br>
 +
up to 100 ml H<sub>2</sub>O</br></br>
 +
</br>
 +
<b>Iron(II) sulfate heptahydrate</b></br>
 +
3.6 mM FeSO<sub>4</sub>*7H<sub>2</sub>O</br>
 +
up to 1L Water</br>
 +
</br>
 +
Adjust to pH=3 after mixing to avoid precipitation.</br>
 +
</br>
 +
<b>Zinc sulfate heptahydrate</b></br>
 +
347.75 mM ZnSO<sub>4</sub>*7H<sub>2</sub>O</br>
 +
up to 100 ml Water</br></br>
 
</p>
 
</p>
  
−
</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
+
<p>
 +
<b>Boric Acid</b></br>
 +
161.73 mM H<sub>3</sub>BO<sub>3</sub></br>
 +
up to 100 ml Water</br></br>
 +
</br>
 +
 
 +
<b>Cobalt(II) chloride hexahydrate</b></br>
 +
42.03mM CoCl<sub>2</sub>*6H<sub>2</sub>O</br>
 +
up to 100 ml H<sub>2</sub>O</br>
 +
</br>
 +
<b>Manganese(II) chloride tetrahydrate</b></br>
 +
50.53 mM MnCl<sub>2</sub>*4H<sub>2</sub>O</br>
 +
up to 100 ml</br>
 +
</br>
 +
<b>Nickel(II) chloride hexahydrate</b></br>
 +
42.07 mM NiCl<sub>2</sub>* 6H<sub>2</sub>O</br>
 +
up to 100 ml Water</br>
 +
</br>
 +
</p>
 +
 
 +
 
 +
<div class="clear"></div>
 +
<!---------------sponsors------------------------->
 +
 
 +
 
 +
 
 +
<center><h1>iGEM UiOslo 2015 is sponsored by:</h1>
 +
<hr style="height:2px;border:none;color:black;background-color:black;" />
 +
<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/e/e0/UiOslo_uio_logo.png" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/4/4a/UiOslo_IDT.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
 +
<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
 +
</center>
 +
<hr style="height:2px;border:none;color:black;background-color:black;" />
 +
 
 +
<!--------end sponsors---------------------------->
 +
 
 +
</body>
 
</html>
 
</html>

Latest revision as of 12:24, 18 September 2015

NMS medium

Back to Protocols


10x NMS
98.9 mM KNO3
43.8 mM MgSO4 * 6 H2O
9.0 mM CaCl2
up to 1L Water


Ethylenediaminetetraacetic acid ferric sodium salt
103.5 mM FeEDTA
up to 100 ml Water


Sodium Molybdate dihydrate
1.07 mM NaMoO4 *2H2O
up to 500 ml Water


Trace Elements solution
0.8 mM CuSO4*5 H2O
1.8 mM FeSO4*7H2O
1.4 mM ZnSO4*7H2O
0.24 mM H3BO3
0.21 mM CoCl2*6H2O
0.74 mM EDTA-Na2
0.1 mM MnCL2*4H2O
42.07 µM NiCl2*6H2O
up to 500 ml Water

Store dark.

Phosphate buffer
198.25 mM Na2HPO4*12H2O
191.05 mM KH2PO4
up to 100 ml Water

Add H2O to 800 ml, adjust pH to 6,8 and add H2O to 1000 ml. Autoclave or sterile filter.

Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate
400.5 mM CuSO4*5H2O
up to 100 ml H2O


Iron(II) sulfate heptahydrate
3.6 mM FeSO4*7H2O
up to 1L Water

Adjust to pH=3 after mixing to avoid precipitation.

Zinc sulfate heptahydrate
347.75 mM ZnSO4*7H2O
up to 100 ml Water

Boric Acid
161.73 mM H3BO3
up to 100 ml Water


Cobalt(II) chloride hexahydrate
42.03mM CoCl2*6H2O
up to 100 ml H2O

Manganese(II) chloride tetrahydrate
50.53 mM MnCl2*4H2O
up to 100 ml

Nickel(II) chloride hexahydrate
42.07 mM NiCl2* 6H2O
up to 100 ml Water

iGEM UiOslo 2015 is sponsored by: