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Latest revision as of 12:32, 18 September 2015
Blue Bio Energy
![](https://static.igem.org/mediawiki/2015/f/f5/Igem.groningen.2015.show.details.transparent.png)
Ligation tasA, Psalt in K823023 (t46)
In this experiment the salt promoter is ligated with tasA in the K823023 backbone.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The ligation of tasA , Psalt and K823023 was performed.
Ligation
00:00, 16 July 2015 - 00:00, 16 July 2015
The samples 1, 2 and 3 from the digestion step were ligated using the following amounts.
Component
Amount
DNA 1 (Psalt)
5 µL
DNA 2 (T2)
5 µL
DNA 3 (K823023)
2.5 µL
T4 ligase
0.5 µL
10x buffer
2 µL
\( \mathrm{H_2O}\)
5 µL
Components ligation sample.
The sample was ligated at room temperature for 30 minutes.