Difference between revisions of "Team:Aalto-Helsinki/LabResults"

(Added more notes to CC and new fig.)
(Deleted Constructs from the sidebar)
 
(39 intermediate revisions by 6 users not shown)
Line 29: Line 29:
  
 
#sidenav > li > a > h3 {
 
#sidenav > li > a > h3 {
 +
  margin-top:1px;
 +
  padding-top: 0px;
 +
  padding-bottom: 0px;
 +
}
 +
#sidenav > li > a > h4 {
 
   margin-top:1px;
 
   margin-top:1px;
 
   padding-top: 0px;  
 
   padding-top: 0px;  
Line 42: Line 47:
 
   #sidenav { display: none; }
 
   #sidenav { display: none; }
 
}
 
}
 +
/* Background pic */
 +
.proodlebg {
 +
    background-repeat: repeat-y;
 +
    background-image: url("https://static.igem.org/mediawiki/2015/a/ab/Aalto-Helsinki_results_background.png");
 +
    background-size: 100%;
 +
}
 
</style>
 
</style>
  
 
       <ul id="sidenav" class="nav nav-stacked"><!-- nav-pills if we want rounded corners -->
 
       <ul id="sidenav" class="nav nav-stacked"><!-- nav-pills if we want rounded corners -->
−
        <li><a href="#modeling" data-scroll="modeling"><h3>Modeling results</h3></a></li>
 
−
        <li><a href="#lab" data-scroll="lab"><h3>Lab results</h3></a></li>
 
−
        <li><a href="#future" data-scroll="future"><h3>Future</h3></a></li>
 
 
         <li><a href="#"><h3 style="border-top:solid;">To the top</h3></a></li>
 
         <li><a href="#"><h3 style="border-top:solid;">To the top</h3></a></li>
 
         <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Results"><h3 >To the Parent Page</h3></a></li>
 
         <li><a href="https://2015.igem.org/Team:Aalto-Helsinki/Results"><h3 >To the Parent Page</h3></a></li>
 
       </ul>
 
       </ul>
  
 +
<div class="proodlebg">
 
<div class="inner-container">
 
<div class="inner-container">
  
  
  
−
<h1>Laboratory Results</h1>
 
  
−
<!-- Modeling results -->
 
−
<section id="modeling" data-ancor="modeling">
 
  
 
</section>
 
</section>
−
<!-- modeling ends -->
 
  
  
−
<section id="lab" data-anchor="lab">
 
  
−
<h2>Continuous production</h2>
+
<p style="margin-bottom:0;padding-bottom:10%;"></p>
  
−
<figure style="float:right;margin-left:30px;">
 
−
  <img src="https://static.igem.org/mediawiki/2015/8/84/Aalto-Helsinki_figure_for_continuous_Growth.PNG" style="max-width: 100%;"/>
 
−
  <figcaption>Figure 1. Population density and dissolved oxygen.</figcaption>
 
−
</figure>
 
  
−
<p>The chemostat cultivation of <i>E.Coli</i>  BL21(DE3) was successful. Batch phase took six hours, and soon after starting the process dissolved oxygen amount decreased into zero. Therefore, oxygen became the limiting factor of growth. Continuous process was started and the first steady state was reached 39 h after starting to feed fresh media into the reactor. With 1,0 l/h aeration, the density of cell population was calculated to be 9,85 g/L at this point, which can be assumed relatively high. Interestingly, bacteria acted as facultative anaerobe when changing its oxygen uptake depending on the growth phase. </br></br> IPTG induction started with 2mM concentration and the population growth reacted right away to changed process conditions. New steady state was achieved 40 h later and the population density became 9,28 g/L. Thus, there was a 0,57 g/L difference of the biomass between the normal growth and the growth with propane production.</p>
 
  
−
<figure style="float:right;margin-left:30px;">
 
−
  <img src="https://static.igem.org/mediawiki/2015/f/f2/Aalto-Helsinki_figure_for_continuous_glucose_ph.PNG " style="max-width: 100%;"/>
 
−
  <figcaption>Figure 2. Glucose content and pH-level.</figcaption>
 
−
</figure>
 
−
 
−
<p>Reactor's glucose consentration decreased from original 20 g/l to zero during the exponential grow phase as the cells used it as a carbon source. However, it was hard to recognize which was the limiting factor of growth and production, glucose or oxygen? During the first steady state, glucose concentration increased when the need for the catabolism of biomass was lower. The end of batch phase, before started feed freash media, was determined from decreased pH-level when the cells produced acids as a response for nutrient deficiency. TB-media used for cultivation contained phosphates which had buffer capacity but it wasn't enough. pH-control was assembled in order to maintain pH-values above 5,0. </p>
 
−
 
−
<p></br> <i>Bacillus</i> </br></br></p>
 
−
 
−
 
−
 
−
</p>
 
−
 
−
 
−
</section>
 
−
 
−
 
−
<section id="future" data-anchor="future">
 
−
 
−
 
−
 
−
</section>
 
  
  
 +
</div>
 
</div>
 
</div>
 
</div></div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
 
</div></div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
−
 
+
<p style="margin-bottom:0;">
  
  
 
<!-- Scirpt for beautiful scrolling inside the page: smooth transitions and we show in what section the reader is. -->
 
<!-- Scirpt for beautiful scrolling inside the page: smooth transitions and we show in what section the reader is. -->
 
<script src="https://2015.igem.org/Team:Aalto-Helsinki/ResponsiveNavScript?action=raw&ctype=text/javascript"></script>
 
<script src="https://2015.igem.org/Team:Aalto-Helsinki/ResponsiveNavScript?action=raw&ctype=text/javascript"></script>
−
 
−
 
−
</body>
 
−
</html>
 

Latest revision as of 13:00, 18 September 2015