Difference between revisions of "Team:IISER Pune/Notebook"

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25^th June 2015

DH5α competent cells were transformed with the following biobricks:

BioBrick	Part	Backbone	Kit plate, Well
BBa_C0080	araC protein coding sequence	pSB1C3	3,11B
BBa_C0012	LacI protein coding sequence	pSB1C3	pSB1C3

The plates were kept at 37°C for 14 hours.

26^th June 2015

Transformation results:

Biobrick	Number of colonies
BBa_C0080	0
BBa_C0012	3

As number of colonies obtained was very low, the transformation of these biobricks was repeated.

27^th June 2015

Transformation results:

Biobrick	Number of colonies
BBa_C0080	3
BBa_C0012	60

Inoculation:

A colony from BBa_C0080 plate was inoculated in 7ml LB media + 7μL Chloramphenicol. Same was done for BBa_C0012. These cultures were kept at 37°C for 12 hours.

28^th June 2015

No growth was observed in both the liquid cultures.

A new colony was inoculated again.

29^th June 2015

Growth observed in the cultures of BBa_C0080 and BBa_C0012. Gylcerol stocks were made for both.

Plasmid isolation was done using Alkaline Lysis protocol for the 2 cultures. (1.5ml culture x 3)

Concentrations obtained from nanodrop:

Sample	Concentration (ng/μL)	A260/A280
BBa_C0080 (1)	3301.8	2.02
BBa_C0080 (2)	3670.9	1.98
BBa_C0080 (3)	2777.6	1.95
BBa_C0012 (1)	4560.0	1.97
BBa_C0012 (2)	4542.1	1.94
BBa_C0012 (3)	5123.5	1.61

A single digest was set for one of each of these plasmids:

(all volumes in μL)	BBa_C0012	BBa_C0080
PstI	0.3	0.3
10x NEBuffer 3	1	1
100x BSA	0.1	0.1
DNA	0.5	0.5
MilliQ H₂0	8.1	8.1
Total Volume	10	10

- Kept at 37°C for 2 hours

- Heat Inactivation: 80°C for 20 minutes

Gel Electrophoresis:

- Digested samples run on a 1% gel.

- Voltage: 100V

- Time run: 50 minutes

Both samples showed an RNA smear. This occurred as the Alkaline Lysis solution I did not have RNAse. After observing this smear, RNAse was added to the solution.

Re-inoculation of the two cultures was done

1^st July 2015

A plasmid isolation using Alkaline lysis was done.

Sample	Concentration (ng/μL)	A260/A280
BBa_C0080 (1)	4548.1	1.98
BBa_C0080 (2)	3411.9	2.01
BBa_C0080 (3)	3492.6	1.98
BBa_C0012 (1)	2258.4	1.99
BBa_C0012 (2)	2442.6	2.00
BBa_C0012 (3)	2691.4	1.97

A digest was set up with a sample of each plasmid and then run on a gel.

The gel showed bands at correct length, but the band intensity was extremely low and did not reflect the concentration obtained on nanodrop. A new method for plasmid isolation was needed.

2^nd July 2015

BBa_K094120 arrived from iGEM Headquarters and was inoculated in 5ml LB media + 5μL Ampicillin. Kept at 37°C for 12 hours.

3^rd July 2015

The liquid culture of BBa_K094120 was plated and kept overnight at 37°C

5^th July 2015

A single colony was inoculated for BBa_K094120. Kept at 37°C for 12 hours

6^th July 2015

Plasmid isolation of BBa_K094120 using Alkaline Lysis:

Sample	Concentration (ng/μL)	A260/A280
BBa_K094120 (1)	1050.8	1.88
BBa_K094120(2)	1493.7	1.95
BBa_K094120 (3)	1365.2	1.97

29^th July 2015

1% gel run with all samples for the 3 plasmids BBa_C0080, BBa_C0012, BBa_K094120.

All bands seen had low intensity which did not correlate to the concentration on nanodrop.

11^th August 2015

Revive glycerol stocks for BBa_C0080, BBa_K094120 and BBa_C0012. A 50 ml culture was inoculated for BBa_C0080 and BBa_C0012 for a Midiprep using Qiagen kit.

Kept at 37°C overnight

12^th August 2015

Midiprep using Qiagen Kit results for BBa_C0080 and BBa_C0012:

Sample	Concentration (ng/μL)	A260/A280
BBa_C0012	7.0	3.62
BBa_C0080	27.8	2.07

Since results obtained were very low, a new “Qiagen spin miniprep” kit was obtained.

17^th August 2015

Primary cultures used to make an overnight 5ml culture of BBa_C0080, BBa_C0012 and BBa_K094120.

18^th August 2015

Qiagen Spin Miniprep protocol followed. Results:

Sample	Concentration (ng/μL)	A260/A280
BBa_K094120	250.7	1.91
BBa_C0080	165.5	1.92
BBa_C0012	195.4	1.92

A digestion was set to check plasmid length:

(all volumes in μL)	BBa_C0080	BBa_C0012	BBa_K094120
DNA	2	2	2
EcoRI	0.2	0.2	0.2
10x EcoRI Buffer	1	1	1
100x BSA	0.1	0.1	0.1
MilliQ H₂0	6.4	6.4	6.4
Total Volume	10	10	10

Gel: 1% gel run at 100V for 40 minutes.

The bands obtained for all 3 plasmids were at the correct length. The uncut plasmid ran faster than cut plasmid.

21^st August 2015

Inoculated BBa_I13504 (GFP, used as reporter in oscillator construct) from previously transformed plate. Kept at 37°C for 12 hours

22^nd August 2015

- Miniprep of BBa_I13504:

Sample	Concentration (ng/μL)	A260/A280
BBa_I13504	122.4	1.94

- Transformation of the biobrick BBa_B0034 (RBS) in DH5α competent cells (Ampicillin antibiotic)

- Plates kept at 37°C for 14 hours

23^rd August 2015

Inoculated a colony for BBa_B0034 and kept at 37°C for 12 hours.

24^th August 2015

Plasmid Isolation using Qiagen Miniprep kit:

Sample	Concentration (ng/μL)	A260/A280
BBa_B0034	95.2	1.95

25^th August 2015

- Transformation of the biobrick BBa_B0015 (double terminator) in DH5α competent cells (Chloramphenicol antibiotic)

- Plates kept at 37°C for 14 hours

26^th August 2015

Transformation Result:

Number of colonies for BBa_B0015 = 8

- Inoculated BBa_B0015 in 5ml LB media + 5 μL Chloramphenicol

- Kept at 37°C for 12 hours

28^th August 2015

Plasmid Isolation of BBa_B0015 using Qiagen Miniprep kit:

Sample	Concentration (ng/μL)	A260/A280
BBa_B0034	72.3	1.95

29^th August 2015

Single Digest to check plasmid length:

(all volumes in μL)	BBa_B0015	BBa_I13504	BBa_B0034
DNA	4	2	2
EcoRI	0.2	0.2	0.2
10x EcoRI Buffer	1	1	1
100x BSA	0.1	0.1	0.1
MilliQ H₂0	5.7	5.7	5.7
Total Volume	10	10	10

Gel: 1% gel run with uncut and cut samples at 100V for 40 minutes.

The 3 single cut bands were at the right length.

6^th September 2015

Digestion for 3A assembly of constructs of the oscillator construct:

(all volumes given are in μL)	BBa_ K094120	BBa_ C0080	BBa_ C0012	BBa_ I13504	BBa_ B0034	BBa_ B0015	Ampicillin Backbone	Chloramphenicol Backbone	Kanamycin Backbone
DNA	3	3	3	3	4	5	10	10	10
10x NEBuffer 2	2.5	2.5	2.5	2.5	2.5	2.5	2.5	2.5	2.5
BSA	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5
EcoRI	0.5	0.5	0.5	-	-	-	0.5	0.5	0.5
XbaI	-	-	-	0.5	0.5	0.5	-	-	-
SpeI	0.5	0.5	0.5	-	-	-	-	-	-
PstI	-	-	-	0.5	0.5	0.5	0.5	0.5	0.5
H₂0	13	13	13	13	12	11	6	6	6
Total	20	20	20	20	20	20	20	20	20

- Kept at 37°C for 5 hours

- Heat Inactivation at 80°C for 20 minutes

- The following devices were to be assembled:

- BBa_K094120 + BBa_B0034 + Chloramphenicol backbone = Device 2a

- BBa_C0080 + BBa_B0015 + Kanamycin backbone = Device 2b

- BBa_C0012 + + BBa_B0015 + Ampicillin backbone = Device 2c

- BBa_K094120 + BBa_I13504 + Kanamycin backbone = Device 2d

Ligation:

(all volumes in μL)	Device 2a	Device 2b	Device 2c	Device 2d
Ampicillin Backbone	-	-	2	-
Chloramphenicol Backbone	2	-	-	-
Kanamycin Backbone	-	2	-	2
BBa_K094120	2	-	-	2
BBa_B0034	2	-	-	-
BBa_C0080	-	2	-	-
BBa_B0015	-	2	2	-
BBa_C0012	-	-	2	-
BBa_I13504	-	-	-	2
T4 DNA ligase	0.5	0.5	0.5	0.5
Ligase Buffer	1	1	1	1
MilliQ H₂0	2.5	2.5	2.5	2.5
Total Volume	20	20	20	20

Ligation kept overnight at room temperature.

7^th September 2015

- Transformation of the ligation mix into DH5α cells

- Kept at 37°C overnight

8^th September 2015

Transformation results:

Ampicillin Negative Control : Lawn growth

Kanamycin Negative Control : Zero colonies

Plate	Number of Colonies
2a	10
2b	12
2c	Lawn growth
2d	5

Ampicillin degraded. 2c re-transformed

Inoculation of a single colony from each plate in 5ml LB media + 5μL antibiotic

Kept at 37°C for 12 hours

9^th September 2015

Miniprep by Qiagen Kit:

Sample	Concentration (ng/μL)	A260/A280
2a	283.4	1.91
2b	122.3	2.09
2d	226.6	1.96

Single Digest with EcoRI to check plasmid length:

Backbone-Backbone ligation was observed on the gel.

A new ligation was setup. This time insert:vector ratio was increased. Kept at 37°C for 2 hours.

These ligation mix were transformed into Dh5α competent cells.

10^th September 2015

Growth observed in negative control plates.

An overnight digestion was set for the individual plasmids again. Kept at 37°C for 12 hours

11^th September 2015

Ligation set for 2 hours and then transformed.

12^th September 2015

Colonies of device 2a, 2b and 2c obtained. No colonies on 2d.

3 colonies from each plate were inoculated, incubated for 12 hours at 37°C and miniprepped.

These were then digested with EcoRI to check the length of the plasmid on a gel.

The bands were as expected.

An overnight digest followed by 2 hour ligation was set to assemble to following device:

Device	Part1	Part2	Backbone
5a	2a	2b	pSB1A3
5b	2a	2c	pSB1K3

Two samples of 5b were assembled – one low copy and second high copy backbone

BBa_I13504 in Ampicillin backbone was chosen to assemble device 2d. This biobrick was digested and ligated.

Devices 5a, 5b and 2d were transformed.

13^th Spetember 2015

Transformation Results:
Negative Control: Zero

Plate	Number of Colonies
5a	15
5b (low copy)	70
5b (high copy)	26
2d	10

Three colonies were inoculated from each plate, incubated at 37°C and then miniprepped.

These were then single digested using EcorI to check on gel.

2d, 5a and 5b high copy showed correct bands.

18^th September 2015

2d construct was characterized by induction with different concentrations of IPTG and arabinose.

@@ Line 19: / Line 19: @@
 </div>
+<body lang=EN-IN style='tab-interval:36.0pt'>
 <div class=WordSection1>
-<p class=MsoNormal><span style='mso-spacerun:yes'> </span><span
+<p class=MsoNormal>25<sup>th</sup> June 2015</p>
-style='mso-tab-count:1'>               </span>25<sup>th</sup> June 2015</p>
 <p class=MsoNormal>DH5&#945; competent cells were transformed with the
@@ Line 124: / Line 125: @@
 <p class=MsoNormal>Transformation results:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>Biobrick</span></p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Number of colonies</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>As number of colonies obtained was very low, the
+transformation of these <span class=SpellE>biobricks</span> was repeated.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>27<sup>th</sup> June 2015</p>
+<p class=MsoNormal>Transformation results:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>Biobrick</span></p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Number of colonies</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>60</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Inoculation:</p>
+<p class=MsoNormal>A colony from BBa_C0080 plate was inoculated in 7ml LB media
++ 7&#956;L Chloramphenicol. Same was done for BBa_C0012. These cultures were
+kept at 37°C for 12 hours.</p>
+<p class=MsoNormal>28<sup>th</sup> June 2015</p>
+<p class=MsoNormal>No growth was observed in both the liquid cultures.</p>
+<p class=MsoNormal>A new colony was inoculated again.</p>
+<p class=MsoNormal>29<sup>th</sup> June 2015</p>
+<p class=MsoNormal>Growth observed in the cultures of BBa_C0080 and BBa_C0012. <span
+class=SpellE>Gylcerol</span> stocks were made for both.</p>
+<p class=MsoNormal>Plasmid isolation was done using Alkaline <span
+class=SpellE>Lysis</span> protocol for the 2 cultures. (1.5ml culture x 3)</p>
+<p class=MsoNormal>Concentrations obtained from <span class=SpellE>nanodrop</span>:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080 (1)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3301.8</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.02</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080 (2)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3670.9</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.98</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080 (3)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2777.6</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.95</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012 (1)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>4560.0</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.97</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012 (2)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>4542.1</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.94</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012 (3)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5123.5</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.61</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>A single digest was set for one of each of these plasmids:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>(all volumes in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>PstI</span></p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.3</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10x <span class=SpellE>NEBuffer</span> 3</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>100x BSA</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>DNA</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>MilliQ</span> H<sub>2</sub>0</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>8.1</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>8.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Total Volume</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoListParagraphCxSpFirst style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Kept at 37°C for 2 hours</p>
+<p class=MsoListParagraphCxSpLast style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Heat Inactivation: 80°C for 20 minutes</p>
+<p class=MsoNormal style='margin-left:18.0pt'><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Gel Electrophoresis:</p>
+<p class=MsoListParagraphCxSpFirst style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Digested samples run on a 1% gel.</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Voltage: 100V</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Time run: 50 minutes</p>
+<p class=MsoListParagraphCxSpLast><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal style='margin-left:18.0pt'>Both samples showed an RNA smear.
+This occurred as the Alkaline <span class=SpellE>Lysis</span> solution I did
+not have <span class=SpellE>RNAse</span>. After observing this smear, <span
+class=SpellE>RNAse</span> was added to the solution<span style='font-family:
+"Times New Roman","serif"'>.<o:p></o:p></span></p>
+<p class=MsoNormal style='margin-left:18.0pt'>Re-inoculation of the two
+cultures was done</p>
+<p class=MsoNormal style='margin-left:18.0pt'><o:p>&nbsp;</o:p></p>
+<span style='font-size:11.0pt;line-height:115%;font-family:"Calibri","sans-serif";
+mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:
+minor-latin;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
+mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-IN;mso-fareast-language:
+EN-US;mso-bidi-language:AR-SA'><br clear=all style='mso-special-character:line-break;
+page-break-before:always'>
+</span>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal style='margin-left:18.0pt'>1<sup>st</sup> July 2015</p>
+<p class=MsoNormal style='margin-left:18.0pt'>A plasmid isolation using <span
+class=GramE>Alkaline</span> <span class=SpellE>lysis</span> was done.</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080 (1)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>4548.1</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.98</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080 (2)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3411.9</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.01</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080 (3)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3492.6</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.98</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012 (1)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2258.4</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.99</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012 (2)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2442.6</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.00</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012 (3)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2691.4</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.97</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal style='margin-left:18.0pt'><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal style='margin-left:18.0pt'>A digest was set up with a sample
+of each plasmid and then run on a gel.</p>
+<p class=MsoNormal style='margin-left:18.0pt'>The gel showed bands at correct
+length, but the band intensity was extremely low and did not reflect the
+concentration obtained on <span class=SpellE>nanodrop</span>. A new method for
+plasmid isolation was needed.</p>
+<p class=MsoNormal style='margin-left:18.0pt'>2<sup>nd</sup> July 2015</p>
+<p class=MsoNormal style='margin-left:18.0pt'>BBa_K094120 arrived from <span
+class=SpellE>iGEM</span> Headquarters and was inoculated in 5ml LB media +
+&#956;L Ampicillin. <span class=GramE>Kept at 37°C for 12 hours.</span></p>
+<p class=MsoNormal style='margin-left:18.0pt'><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal style='margin-left:18.0pt'>3<sup>rd</sup> July 2015</p>
+<p class=MsoNormal>The liquid culture of BBa_K094120 was plated and kept
+overnight at 37°C </p>
+<p class=MsoNormal>5<sup>th</sup> July 2015</p>
+<p class=MsoNormal>A single colony was inoculated for BBa_K094120. Kept at 37°C
+for 12 hours</p>
+<p class=MsoNormal>6<sup>th</sup> July 2015</p>
+<p class=MsoNormal>Plasmid isolation of BBa_K094120 using Alkaline <span
+class=SpellE>Lysis</span>:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_K094120 (1)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1050.8</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.88</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_K094120(2)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1493.7</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.95</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_K094120 (3)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1365.2</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.97</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>29<sup>th</sup> July 2015</p>
+<p class=MsoNormal>1% gel run with all samples for the 3 plasmids BBa_C0080,
+BBa_C0012, BBa_K094120.</p>
+<p class=MsoNormal>All bands seen had low intensity which did not correlate to
+the concentration on <span class=SpellE>nanodrop</span>.</p>
+<span style='font-size:11.0pt;line-height:115%;font-family:"Calibri","sans-serif";
+mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:
+minor-latin;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
+mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-IN;mso-fareast-language:
+EN-US;mso-bidi-language:AR-SA'><br clear=all style='mso-special-character:line-break;
+page-break-before:always'>
+</span>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>11<sup>th</sup> August 2015</p>
+<p class=MsoNormal>Revive glycerol stocks for BBa_C0080, BBa_K094120 and
+BBa_C0012. A 50 ml culture was inoculated for BBa_C0080 and BBa_C0012 for a <span
+class=SpellE>Midiprep</span> using <span class=SpellE>Qiagen</span> kit.</p>
+<p class=MsoNormal>Kept at 37°C overnight</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>12<sup>th</sup> August 2015</p>
+<p class=MsoNormal><span class=SpellE>Midiprep</span> using <span class=SpellE>Qiagen</span>
+Kit results for BBa_C0080 and BBa_C0012:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>7.0</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3.62</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>27.8</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.07</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Since results obtained were very low, a new “<span
+class=SpellE>Qiagen</span> spin <span class=SpellE>miniprep</span>” kit was
+obtained.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>17<sup>th</sup> August 2015</p>
+<p class=MsoNormal>Primary cultures used to make an overnight 5ml culture of
+BBa_C0080, BBa_C0012 and BBa_K094120.</p>
+<p class=MsoNormal>18<sup>th</sup> August 2015</p>
+<p class=MsoNormal><span class=SpellE>Qiagen</span> Spin <span class=SpellE>Miniprep</span>
+protocol followed. Results:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_K094120</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>250.7</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.91</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>165.5</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.92</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>195.4</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.92</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>A digestion was set to check plasmid length:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>(all volumes in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_K094120</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>DNA</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>EcoRI</span></p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10x <span class=SpellE>EcoRI</span> Buffer</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>100x BSA</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>MilliQ</span> H<sub>2</sub>0</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>6.4</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>6.4</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>6.4</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6;mso-yfti-lastrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Total Volume</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Gel: 1% gel run at 100V for 40 minutes.</p>
+<p class=MsoNormal>The bands obtained for all 3 plasmids were at the correct
+length. The uncut plasmid ran faster than cut plasmid.</p>
+<p class=MsoNormal>21<sup>st</sup> August 2015</p>
+<p class=MsoNormal>Inoculated BBa_I13504 (GFP, used as reporter in oscillator
+construct) from previously transformed plate. Kept at 37°C for 12 hours</p>
+<p class=MsoNormal>22<sup>nd</sup> August 2015</p>
+<p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]><span class=SpellE>Miniprep</span> of
+BBa_I13504:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='margin-left:36.0pt;border-collapse:collapse;border:none;mso-border-alt:
+ solid windowtext .5pt;mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=189 valign=top style='width:142.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=189 valign=top style='width:142.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=189 valign=top style='width:142.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1;mso-yfti-lastrow:yes'>
+  <td width=189 valign=top style='width:142.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoListParagraphCxSpFirst style='margin:0cm;margin-bottom:.0001pt;
+  mso-add-space:auto;line-height:normal'>BBa_I13504</p>
+  </td>
+  <td width=189 valign=top style='width:142.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoListParagraphCxSpMiddle style='margin:0cm;margin-bottom:.0001pt;
+  mso-add-space:auto;line-height:normal'>122.4</p>
+  </td>
+  <td width=189 valign=top style='width:142.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoListParagraphCxSpLast style='margin:0cm;margin-bottom:.0001pt;
+  mso-add-space:auto;line-height:normal'>1.94</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoListParagraphCxSpFirst><o:p>&nbsp;</o:p></p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Transformation of the <span class=SpellE>biobrick</span>
+BBa_B0034 (RBS) in DH5&#945; competent cells (Ampicillin antibiotic)</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Plates kept at 37°C for 14 hours</p>
+<p class=MsoListParagraphCxSpLast><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>23<sup>rd</sup> August 2015</p>
+<p class=MsoNormal>Inoculated a colony for BBa_B0034 and kept at 37°C for 12
+hours.</p>
+<p class=MsoNormal>24<sup>th</sup> August 2015</p>
+<p class=MsoNormal>Plasmid Isolation using <span class=SpellE>Qiagen</span> <span
+class=SpellE>Miniprep</span> kit:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_B0034</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>95.2</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.95</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>25<sup>th</sup> August 2015</p>
+<p class=MsoListParagraphCxSpFirst style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Transformation of the <span class=SpellE>biobrick</span>
+BBa_B0015 (double terminator) in DH5&#945; competent cells (Chloramphenicol
+antibiotic)</p>
+<p class=MsoListParagraphCxSpLast style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Plates kept at 37°C for 14 hours</p>
+<p class=MsoNormal>26<sup>th</sup> August 2015</p>
+<p class=MsoNormal>Transformation Result:</p>
+<p class=MsoNormal>Number of colonies for BBa_B0015 = 8</p>
+<p class=MsoListParagraphCxSpFirst style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Inoculated BBa_B0015 in 5ml LB media + 5 <span
+class=SpellE>&#956;L</span> Chloramphenicol</p>
+<p class=MsoListParagraphCxSpLast style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Kept at 37°C for 12 hours</p>
+<p class=MsoNormal>28<sup>th</sup> August 2015</p>
+<p class=MsoNormal>Plasmid Isolation of BBa_B0015 using <span class=SpellE>Qiagen</span>
+<span class=SpellE>Miniprep</span> kit:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_B0034</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>72.3</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.95</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>29<sup>th</sup> August 2015</p>
+<p class=MsoNormal>Single Digest to check plasmid length:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>(all volumes in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_B0015</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_I13504</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_B0034</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>DNA</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>4</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>EcoRI</span></p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10x <span class=SpellE>EcoRI</span> Buffer</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>100x BSA</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>MilliQ</span> H<sub>2</sub>0</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5.7</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5.7</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5.7</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6;mso-yfti-lastrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Total Volume</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Gel: 1% gel run with uncut and cut samples at 100V for 40
+minutes.</p>
+<p class=MsoNormal>The 3 single cut bands were at the right length.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>6<sup>th</sup> September 2015</p>
+<p class=MsoNormal><span class=GramE>Digestion for 3A assembly of constructs of
+the oscillator construct</span>:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0 width=747
+ style='width:559.95pt;margin-left:-51.3pt;border-collapse:collapse;border:
+ none;mso-border-alt:solid windowtext .5pt;mso-yfti-tbllook:1184;mso-padding-alt:
+cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>(all volumes given are in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'><span class=SpellE>BBa</span>_</p>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>K094120</p>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'><o:p>&nbsp;</o:p></p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'><span class=SpellE>BBa</span>_</p>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>C0080</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'><span class=SpellE>BBa</span>_</p>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>C0012</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'><span class=SpellE>BBa</span>_</p>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>I13504</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'><span class=SpellE>BBa</span>_</p>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>B0034</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'><span class=SpellE>BBa</span>_</p>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>B0015</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>Ampicillin Backbone</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>Chloramphenicol Backbone</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+  text-align:center;line-height:normal'>Kanamycin Backbone</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>DNA</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>3</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>4</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10x <span class=SpellE>NEBuffer</span> 2</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BSA</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>EcoRI</span></p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>XbaI</span></p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>SpeI</span></p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:7'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>PstI</span></p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:8'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>H<sub>2</sub>0</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>13</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>13</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>13</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>13</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>12</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>11</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>6</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>6</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>6</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:9;mso-yfti-lastrow:yes'>
+  <td width=76 valign=top style='width:2.0cm;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Total</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=66 valign=top style='width:49.6pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=57 valign=top style='width:42.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=57 valign=top style='width:42.5pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=76 valign=top style='width:2.0cm;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=123 valign=top style='width:92.15pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=85 valign=top style='width:63.8pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoListParagraphCxSpFirst style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Kept at 37°C for 5 hours</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Heat Inactivation at 80°C for 20 minutes</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>The following devices were to be assembled:</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>BBa_K094120 + BBa_B0034 + Chloramphenicol
+backbone = Device 2a</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>BBa_C0080 + BBa_B0015 + Kanamycin backbone =
+Device 2b</p>
+<p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>BBa_C0012 + + BBa_B0015 + Ampicillin backbone =
+Device 2c</p>
+<p class=MsoListParagraphCxSpLast style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>BBa_K094120 + BBa_I13504 + Kanamycin backbone =
+Device 2d</p>
+<p class=MsoNormal style='margin-left:18.0pt'><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal style='margin-left:18.0pt'><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Ligation:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='margin-left:-37.15pt;border-collapse:collapse;border:none;mso-border-alt:
+ solid windowtext .5pt;mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>(all volumes in <span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Device 2a</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Device 2b</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Device 2c</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Device 2d</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:0cm;
+  margin-left:15.85pt;margin-bottom:.0001pt;line-height:normal'>Ampicillin
+  Backbone</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Chloramphenicol Backbone</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Kanamycin Backbone</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_K094120</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:5'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_B0034</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:6'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0080</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:7'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_B0015</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:8'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_C0012</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:9'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>BBa_I13504</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>-</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:10'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>T4 DNA ligase</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>0.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:11'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Ligase Buffer</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:12'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'><span class=SpellE>MilliQ</span> H<sub>2</sub>0</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.5</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:13;mso-yfti-lastrow:yes'>
+  <td width=180 valign=top style='width:134.7pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Total Volume</p>
+  </td>
+  <td width=116 valign=top style='width:87.25pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=123 valign=top style='width:92.4pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+  <td width=123 valign=top style='width:92.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>20</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Ligation kept overnight at room temperature.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>7<sup>th</sup> September 2015</p>
+<p class=MsoListParagraphCxSpFirst style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Transformation of the ligation mix into
+DH5&#945; cells</p>
+<p class=MsoListParagraphCxSpLast style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span
+style='mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;
+mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri'><span
+style='mso-list:Ignore'>-<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></span></span><![endif]>Kept at 37°C overnight</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>8<sup>th</sup> September 2015</p>
+<p class=MsoNormal>Transformation results:</p>
+<p class=MsoNormal>Ampicillin Negative <span class=GramE>Control :</span> Lawn
+growth</p>
+<p class=MsoNormal>Kanamycin Negative <span class=GramE>Control :</span> Zero
+colonies</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Plate</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Number of Colonies</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2a</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2b</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>12</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2c</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Lawn growth</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4;mso-yfti-lastrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2d</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Ampicillin degraded. 2c re-transformed</p>
+<p class=MsoNormal>Inoculation of a single colony from each plate in 5ml LB
+media + 5&#956;L antibiotic</p>
+<p class=MsoNormal>Kept at 37°C for 12 hours</p>
+<p class=MsoNormal>9<sup>th</sup> September 2015</p>
+<p class=MsoNormal><span class=SpellE>Miniprep</span> by <span class=SpellE>Qiagen</span>
+Kit:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Sample</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Concentration (<span class=SpellE>ng</span>/<span class=SpellE>&#956;L</span>)</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>A260/A280</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2a</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>283.4</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.91</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2b</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>122.3</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2.09</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes'>
+  <td width=205 valign=top style='width:154.0pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2d</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>226.6</p>
+  </td>
+  <td width=205 valign=top style='width:154.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>1.96</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Single Digest with <span class=SpellE>EcoRI</span> to check
+plasmid length:</p>
+<p class=MsoNormal>Backbone-Backbone ligation was observed on the gel.</p>
+<p class=MsoNormal>A new ligation was setup. This time <span class=SpellE>insert<span
+class=GramE>:vector</span></span> ratio was increased. <span class=GramE>Kept
+at 37°C for 2 hours.</span></p>
+<p class=MsoNormal>These ligation <span class=GramE>mix</span> were transformed
+into Dh5&#945; competent cells.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>10<sup>th</sup> September 2015</p>
+<p class=MsoNormal>Growth observed in negative control plates.</p>
+<p class=MsoNormal>An overnight digestion was set for the individual plasmids
+again. Kept at 37°C for 12 hours</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>11<sup>th</sup> September 2015</p>
+<p class=MsoNormal>Ligation set for 2 hours and then transformed.</p>
+<p class=MsoNormal>12<sup>th</sup> September 2015</p>
+<p class=MsoNormal>Colonies of device 2a, 2b and 2c obtained. No colonies on
+d.</p>
+<p class=MsoNormal>3 colonies from each plate were inoculated, incubated for 12
+hours at 37°C and miniprepped.</p>
+<p class=MsoNormal>These were then digested with <span class=SpellE>EcoRI</span>
+to check the length of the plasmid on a gel.</p>
+<p class=MsoNormal>The bands were as expected.</p>
+<p class=MsoNormal>An overnight digest followed by 2 hour ligation was set to
+assemble to following device:</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Device</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Part1</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Part2</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Backbone</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5a</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2a</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2b</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>pSB1A3</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes'>
+  <td width=154 valign=top style='width:115.5pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5b</p>
+  </td>
+  <td width=154 valign=top style='width:115.5pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2a</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2c</p>
+  </td>
+  <td width=154 valign=top style='width:115.55pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>pSB1K3</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Two samples of 5b were assembled – one low copy and second high
+copy backbone</p>
+<p class=MsoNormal>BBa_I13504 in Ampicillin backbone was chosen to assemble
+device 2d. This <span class=SpellE>biobrick</span> was digested and ligated.</p>
+<p class=MsoNormal>Devices 5a, 5b and 2d were transformed.</p>
+<p class=MsoNormal>13<sup>th</sup> <span class=SpellE>Spetember</span> 2015</p>
+<p class=MsoNormal>Transformation Results:<br>
+<span style='mso-tab-count:1'>                </span>Negative Control: Zero</p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none;mso-border-alt:solid windowtext .5pt;
+ mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+ <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Plate</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>Number of Colonies</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:1'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5a</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>15</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:2'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5b (low copy)</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>70</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:3'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>5b (high copy)</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>26</p>
+  </td>
+ </tr>
+ <tr style='mso-yfti-irow:4;mso-yfti-lastrow:yes'>
+  <td width=308 valign=top style='width:231.05pt;border:solid windowtext 1.0pt;
+  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+  padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>2d</p>
+  </td>
+  <td width=308 valign=top style='width:231.05pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
+  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+  normal'>10</p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>Three colonies were inoculated from each plate, incubated at
+°C and then <span class=SpellE>miniprepped</span>.</p>
+<p class=MsoNormal>These were then single digested using <span class=SpellE>EcorI</span>
+to check on gel.</p>
+<p class=MsoNormal>2d, 5a and 5b high copy showed correct bands.</p>
+<p class=MsoNormal><o:p>&nbsp;</o:p></p>
+<p class=MsoNormal>18<sup>th</sup> September 2015</p>
+<p class=MsoNormal>2d construct was characterized by induction with different
+concentrations of IPTG and arabinose.</p>
+</div>
+</body>
 </html>