Difference between revisions of "Team:ANU-Canberra/labbook"

 
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<h2>Our Project</h2>
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<h2>Our Lab Book</h2>
 
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<h3>Blue light source</h3>
 
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<h3>Cre-recombinase functionality assay</h3>
 
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<p>We are taking part in the Foundational Advance track, exploring the principles and application of light-induction as an alternative to traditional chemical induction.</p>
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<p>We tested the functionality of the light-activated CRY2/CIB1-Cre-recombinase system using a reporter loxP construct.
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<h3>FRET Tests</h3>
 
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<p>Safe use of genetically engineered machines involves physical containment, safe practices as well as biocontainment methods to prevent escape, survival and proliferation of engineered microorganisms in natural environments.</p>
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<p>As a proof of principle for the expressability and association of the CRY2-CIB1 optogenetic system, we sought to conduct a fluorescence assay based upon the Forster resonance energy transfer (FRET) phenomenon.</p>
 
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<p>This year being The International Year of Light and Light-based Technologies, we were inspired to investigate potential uses of light in synthetic biology. One such application is optogenetics.  Optogenetics is defined by the control of cellular dynamics using light. It allows for the ability to control the translocation of proteins, transcriptional activation and other potential uses.</p>
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<p>Even a successfully expressed protein may not function properly - solubility of our product was one indicator of how successful our light-inducible system would be.</p>
 
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<p>We are taking part in the Foundational Advance track, exploring the principles and application of light-induction as an alternative to traditional chemical induction.</p>
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<p>A documentation of experiments we have run from the beginning to the end of our project.</p>
 
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<p>Safe use of genetically engineered machines involves physical containment, safe practices as well as biocontainment methods to prevent escape, survival and proliferation of engineered microorganisms in natural environments.</p>
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Latest revision as of 13:53, 18 September 2015

Our Lab Book

Blue light source

We have devised a simple method to create your own light source from cheap, readily accessible materials, that can be customised for light intensity and pulse frequency - with minimal prior knowledge of electric circuits or experience with building them.

Cre-recombinase functionality assay

We tested the functionality of the light-activated CRY2/CIB1-Cre-recombinase system using a reporter loxP construct.

FRET Tests

As a proof of principle for the expressability and association of the CRY2-CIB1 optogenetic system, we sought to conduct a fluorescence assay based upon the Forster resonance energy transfer (FRET) phenomenon.

Solubility Tests

Even a successfully expressed protein may not function properly - solubility of our product was one indicator of how successful our light-inducible system would be.

Lab Notes

A documentation of experiments we have run from the beginning to the end of our project.