Difference between revisions of "Team:ANU-Canberra/labbook"

 
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<p>Addressing the industrial aspect of our, we investigated options for potential bioreactors for bulk synthesis of our target material - NAD. </p>
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<p>We tested the functionality of the light-activated CRY2/CIB1-Cre-recombinase system using a reporter loxP construct.
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<p>Safe use of genetically engineered machines involves physical containment, safe practices as well as biocontainment methods to prevent escape, survival and proliferation of engineered microorganisms in natural environments.</p>
 
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Latest revision as of 13:53, 18 September 2015

Our Lab Book

Blue light source

We have devised a simple method to create your own light source from cheap, readily accessible materials, that can be customised for light intensity and pulse frequency - with minimal prior knowledge of electric circuits or experience with building them.

Cre-recombinase functionality assay

We tested the functionality of the light-activated CRY2/CIB1-Cre-recombinase system using a reporter loxP construct.

FRET Tests

As a proof of principle for the expressability and association of the CRY2-CIB1 optogenetic system, we sought to conduct a fluorescence assay based upon the Forster resonance energy transfer (FRET) phenomenon.

Solubility Tests

Even a successfully expressed protein may not function properly - solubility of our product was one indicator of how successful our light-inducible system would be.

Lab Notes

A documentation of experiments we have run from the beginning to the end of our project.