Difference between revisions of "Team:LZU-China/chz safety"

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          <a href=" https://2015.igem.org/Team:LZU-China/chz_collaborations">Collaboration</a>
 
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<div class="col s12"><h3>&nbsp;</h3></div>
 
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<h5> We have taken safety courses before so the entire group members know how to protect ourselves in experiments.  
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<p>Our <week>team determined to make</week> all aspects of the entire project perfect, especially biosafety. The one and the only organisms we used is Escherichia coli whose lab strains that are not harmful to human at all. The main risks in our experiment is the heavy metal we used to exam our parts. To reduce that, we do many experiments in draught cupboards and we use latex gloves and masks to protect ourselves. </p>
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         As for environment, some of the masses hesitate it may cause genetic pollution to the environment, but it should be noticed that the gene modified E.coli we construct cannot express our target gene unless it is stimulated by the pollutant PNP.</h5>
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<p >Our device is designed of a safer future. We design a MFC-based device. The bacteria can sense many kinds of ions and it can improve the voltage in MFC. We modify the E.coli to make it can produce riboflavin when sensed ions. Our device is designed as an enclosed systems. The measurement of the wastewater is safe, and neither the genetically modified sensor cells nor the wastewater is impossible to leak at all. Our device is designed for industrial purpose, and it is really cheap. </p>
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                  <img src="https://static.igem.org/mediawiki/2015/thumb/b/b7/LZU_China_Notes_Safty_MFC_and_AAS.png/784px-LZU_China_Notes_Safty_MFC_and_AAS.png" >
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                  <p>Fig.1 Comparison of MFC and AAS</p>
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                  <a href="#">This is a link</a>
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<p>Our device can be very helpful in many fields. For more information, please contacted with Xiangkai Li </p>
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            <th>&nbsp;</th>
 
−
            <th>Species</th>
 
−
            <th>Strain </th>
 
−
            <th>Risk Group</th>
 
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            <th>Disease    risk to humans? If so, which disease</th>
 
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          <tr>
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            <td>&nbsp;1</td>
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            <th>Part number</th>
−
            <td><em>E. coli</em>K 12</td>
+
            <th>Part number</th>
−
            <td>DH5 alpha</td>
+
            <th>Where did us get the physical DNA for this part </th>
−
            <td><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></td>
+
            <th>What is the Risk Group of the species?</th>
−
            <td>Yes. May    cause irritation to skin, eyes, and respiratory tract, may affect kidneys.    However, E. coli K-12 is not considered a human or animal pathogen nor is it   toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated    by its poor ability to colonize the colon and establish infections.</td>
+
            <th>How will you use it?</th>
 
          </tr>
 
          </tr>
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          <tr>
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        <tr>
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            <td>&nbsp;2</td>
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            <th>1</th>
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            <td><em>Shewenella    Onedensis </em></td>
+
            <th>pSB1C3</th>
−
            <td>MR1</td>
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            <th>From Li Xiangkai's Lab, Lanzhou University </th>
−
            <td><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></td>
+
            <th>1</th>
−
            <td>No, S. oneidensis MR-1 belongs to a class of bacteria known as "Dissimilatory Metal-Reducing Bacteria (DMRB)" because of their ability to couple metal reduction with their metabolism. It cannot cause diseases</td>
+
            <th>Vector for E.coli to express BBA_K1755301 Submission vector for all parts</th>
 
          </tr>
 
          </tr>
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            <th>2</th>
−
 
+
            <th>K1755001</th>
−
 
+
            <th>Synthetize</th>
−
<div class="col s12"><h3>&nbsp;</h3></div>
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            <th>1</th>
−
 
+
            <th>Every parts' foundation</th>
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            <th>Part    number</th>
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            <th>Where did    us get the physical DNA for this part</th>
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            <th>What    species does this part originally come from?</th>
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            <th>&nbsp;Natural    function of part</th>
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            <th>What is    the Risk Group of the species?</th>
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            <th>How will    you use it?</th>
+
 
          </tr>
 
          </tr>
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          <tbody>
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            <th>3</th>
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          <tr>
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            <th>K1755002</th>
−
            <td >&nbsp;1</td>
+
            <th>Parts construction</th>
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            <td >pSB1C3</td>
+
            <th>1</th>
−
            <td>From Li    Xiangkai's Lab, Lanzhou    University</td>
+
            <th>Foundation of other parts</th>
−
            <td><em>Escherichia coli</em> K-12</p></td>
+
−
            <td>Vector for <em>Escherichia coli</em></td>
+
−
            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
+
−
            <td><ol>
+
−
              <li>Vector for <em>E.coli </em>to express BBA_K1523101</li>
+
−
              <li>Submission    vector for all parts </li>
+
−
            </ol></td>
+
 
          </tr>
 
          </tr>
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          <tr>
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          <tr>
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            <td >&nbsp;2</td>
+
            <th>4</th>
−
            <td><a href="http://parts.igem.org/Part:BBa_K1523101">K1523101</a></p></td>
+
            <th>K1755003</th>
−
            <td>From    distributed kit of iGEM 2013<br>
+
            <th>Parts construction</th>
−
              BBA­_K381001</td>
+
            <th>1</th>
−
            <td><em>Escherichia coli</em> K-12 </td>
+
            <th>Foundation of other parts</th>
−
            <td>Detector    of Nitrate</td>
+
−
            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</td>
+
−
            <td>1.&nbsp; Strain used in anode of MFC 2.&nbsp; Detector of PNP<br>
+
−
              3.&nbsp; Riboflavin producing</td>
+
 
          </tr>
 
          </tr>
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          <tr>
+
          <tr>
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            <td >&nbsp;3</td>
+
            <th>5</th>
−
            <td>pET-28(a)    +</td>
+
            <th>K1755004</th>
−
            <td>From Li    Xiangkai's Lab, Lanzhou    University</td>
+
            <th>Parts construction</th>
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            <td>Vector for <em>Escherichia coli</em></td>
+
            <th>1</th>
−
            <td>Vector for <em>Escherichia coli</em></td>
+
            <th>Testing riboflavin expression quantity</th>
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            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</td>
+
−
            <td>Vector for <em>E.coli</em> to express BBA_K1523101</td>
+
 
          </tr>
 
          </tr>
 
          <tr>
 
          <tr>
−
            <td >&nbsp;4</td>
+
            <th>6</th>
−
            <td>BBa_K1523008</td>
+
            <th>K1755005</th>
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            <td>Lanzhou University </td>
+
            <th>Parts construction</th>
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            <td><em>Escherichia coli</em></td>
+
            <th>1</th>
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            <td><p align="left">Chromate    reduction</td>
+
            <th>Testing riboflavin expression quantity</th>
−
            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</td>
+
          <tr>
−
            <td>1.&nbsp; Strain used in anode of MFC 2.&nbsp; Chromate reduction in anode</td>
+
          <th>7</th>
 +
            <th>K1755006</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>8</th>
 +
            <th>K1755007</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>9</th>
 +
            <th>K1755008</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>10</th>
 +
            <th>K1755009</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>11</th>
 +
            <th>K1755301</th>
 +
            <th>Synthetize</th>
 +
            <th>1</th>
 +
            <th>Used in MFC</th>
 
          </tr>
 
          </tr>
 
          <tr>
 
          <tr>
−
            <td>&nbsp;5</td>
+
          <th>12</th>
−
            <td>BBa_K1523021</td>
+
            <th>K1755302</th>
−
            <td>Lanzhou University </td>
+
            <th>Synthetize</th>
−
            <td><em>Pseudomonas fluorescens</em></td>
+
            <th>1</th>
−
            <td><p align="left">Chromate    reduction</td>
+
            <th>Used in MFC</th>
−
            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</td>
+
−
            <td>1.&nbsp; Strain used in cathode of MFC <br>
+
−
              2.&nbsp; Other organic pollutant reduction in    cathode</td>
+
 
          </tr>
 
          </tr>
 
          <tr>
 
          <tr>
−
            <td>&nbsp;6</td>
+
          <th>13</th>
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            <td>BBa_K1523022</td>
+
            <th>K1755303</th>
−
            <td>Lanzhou University </td>
+
            <th>Synthetize</th>
−
            <td><em>Pseudomonas fluorescens</em></td>
+
            <th>1</th>
−
            <td>Chromate    reduction</td>
+
            <th>Used in MFC</th>
−
            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</td>
+
−
            <td><p align="left">1.&nbsp; Strain used in cathode of MFC <br>
+
−
              2.&nbsp; Other organic pollutant reduction in    cathode</td>
+
 
          </tr>
 
          </tr>
 
          <tr>
 
          <tr>
−
            <td>&nbsp;7</td>
+
          <th>14</th>
−
            <td>BBa_K1523023</td>
+
            <th>K1755305</th>
−
            <td>Lanzhou University </td>
+
            <th>Synthetize</th>
−
            <td><em>Pseudomonas fluorescens</em></td>
+
            <th>1</th>
−
            <td><p align="left">Chromate    reduction</td>
+
            <th>Used in MFC</th>
−
            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</td>
+
−
            <td>1.&nbsp; Strain used in cathode of MFC <br>
+
−
              2.&nbsp; Other organic pollutant reduction in    cathode</td>
+
 
          </tr>
 
          </tr>
 
          <tr>
 
          <tr>
−
            <td>&nbsp;8</td>
+
          <th>15</th>
−
            <td>BBa_K1523025</td>
+
            <th>K1755024</th>
−
            <td>Lanzhou University </td>
+
            <th>Parts construction</th>
−
            <td><em>Pseudomonas </em><em>putida</em></td>
+
            <th>1</th>
−
            <td>Chromate    reduction</td>
+
            <th>Used in MFC</th>
−
            <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</td>
+
−
            <td>1.&nbsp; Strain used in anode of MFC 2.&nbsp; Chromate reduction in anode</td>
+
 
          </tr>
 
          </tr>
 +
 
        </tbody>
 
        </tbody>
 
</table>
 
</table>
 +
</h6>
 +
 +
<div class="col s12"><h3>&nbsp;</h3></div>
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Latest revision as of 15:37, 18 September 2015

Team:LZU 2015

 

Safety

(subtitle)this is our principle

 

 

Our team determined to make all aspects of the entire project perfect, especially biosafety. The one and the only organisms we used is Escherichia coli whose lab strains that are not harmful to human at all. The main risks in our experiment is the heavy metal we used to exam our parts. To reduce that, we do many experiments in draught cupboards and we use latex gloves and masks to protect ourselves.

Our device is designed of a safer future. We design a MFC-based device. The bacteria can sense many kinds of ions and it can improve the voltage in MFC. We modify the E.coli to make it can produce riboflavin when sensed ions. Our device is designed as an enclosed systems. The measurement of the wastewater is safe, and neither the genetically modified sensor cells nor the wastewater is impossible to leak at all. Our device is designed for industrial purpose, and it is really cheap.


Fig.1 Comparison of MFC and AAS

Our device can be very helpful in many fields. For more information, please contacted with Xiangkai Li




Part number Part number Where did us get the physical DNA for this part What is the Risk Group of the species? How will you use it?
1 pSB1C3 From Li Xiangkai's Lab, Lanzhou University 1 Vector for E.coli to express BBA_K1755301 Submission vector for all parts
2 K1755001 Synthetize 1 Every parts' foundation
3 K1755002 Parts construction 1 Foundation of other parts
4 K1755003 Parts construction 1 Foundation of other parts
5 K1755004 Parts construction 1 Testing riboflavin expression quantity
6 K1755005 Parts construction 1 Testing riboflavin expression quantity
7 K1755006 Parts construction 1 Testing riboflavin expression quantity
8 K1755007 Parts construction 1 Testing riboflavin expression quantity
9 K1755008 Parts construction 1 Testing riboflavin expression quantity
10 K1755009 Parts construction 1 Testing riboflavin expression quantity
11 K1755301 Synthetize 1 Used in MFC
12 K1755302 Synthetize 1 Used in MFC
13 K1755303 Synthetize 1 Used in MFC
14 K1755305 Synthetize 1 Used in MFC
15 K1755024 Parts construction 1 Used in MFC