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Latest revision as of 15:48, 18 September 2015
Blue Bio Energy
![](https://static.igem.org/mediawiki/2015/f/f5/Igem.groningen.2015.show.details.transparent.png)
tasA PliaG Ligation (t64)
The PliaG promoter is ligated with tasA instead of the salt promoter, as this did not work.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
PliaG was ligated with T2 (tasA).
Ligation
13:22, 6 August 2015 - 18:40, 6 August 2015
The ligation was carried out overnight at room temperature using the following components.
Component
Amount
PliaG DNA
5 µL
T2 DNA
10 µL
10x T4 buffer
2 µL
T4 ligase
0.5 µL
\( \mathrm{H_2O}\)
2.5 µL
Components for ligation of PliaG with T2.