Difference between revisions of "Template:IONIS Paris/Notebook/18-09-15"
(Created page with " {{IONIS_Paris/CSS/Notebook}} <html> <body> <section id="cd-timeline" class="cd-container"> <div class="cd-timeline-block" id="18-09-15"> <div class="cd-timel...") |
|||
| Line 23: | Line 23: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
<p> | <p> | ||
<i class="fa fa-angle-right"></i> The band for the third well is good. WE GOT IT !!!!! <br> | <i class="fa fa-angle-right"></i> The band for the third well is good. WE GOT IT !!!!! <br> | ||
| + | </p> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <p> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2015/b/b8/Graph-180915.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br> | ||
| + | </p> | ||
| + | <p> | ||
| + | <i class="fa fa-angle-right"></i> Induction of BBa_K325909 and BBa_K1159001 with a solution of arabinose 1%, and a solution of IPTG 1 mM for T7-Nanoluc (should correspond to the effect of a constitutive promoter like T7). Under these conditions, light intensity of T7-nanoluc was 1,2 time higher than the BBa_K1159001. <br> | ||
</p> | </p> | ||
| + | |||
| + | <center><img src="https://static.igem.org/mediawiki/2015/a/aa/Graph-180915_2.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br> | ||
| + | </p> | ||
| + | <p> | ||
| + | <i class="fa fa-angle-right"></i> Effect of the concentration of IPTG inducer to the light intensity of T7-nanoluc part. While the concentration of IPTG does not exceed 1 mM, the light intensity remains low. <br> | ||
| + | </p> | ||
| + | |||
| + | <center><img src="https://static.igem.org/mediawiki/2015/8/80/Graph-180915_3.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br> | ||
| + | </p> | ||
| + | <p> | ||
| + | <i class="fa fa-angle-right"></i> Effect of the concentration of arabinose inducer to the light intensity of BBa_K1159001 part. Light intensity is linked to inducer concentration however over 1% of arabinose (final concentration), the intensity not rise anymore. | ||
| + | </p> | ||
| + | |||
<!-- DATE de l'experience !ID aussi --> | <!-- DATE de l'experience !ID aussi --> | ||
Revision as of 15:56, 18 September 2015
Expected results
Results
The band for the third well is good. WE GOT IT !!!!!
Induction of BBa_K325909 and BBa_K1159001 with a solution of arabinose 1%, and a solution of IPTG 1 mM for T7-Nanoluc (should correspond to the effect of a constitutive promoter like T7). Under these conditions, light intensity of T7-nanoluc was 1,2 time higher than the BBa_K1159001.
Effect of the concentration of IPTG inducer to the light intensity of T7-nanoluc part. While the concentration of IPTG does not exceed 1 mM, the light intensity remains low.
Effect of the concentration of arabinose inducer to the light intensity of BBa_K1159001 part. Light intensity is linked to inducer concentration however over 1% of arabinose (final concentration), the intensity not rise anymore.
18 Sept. 15