Difference between revisions of "Template:IONIS Paris/Notebook/18-09-15"
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<i class="fa fa-angle-right"></i> The band for the third well is good. WE GOT IT !!!!! <br> | <i class="fa fa-angle-right"></i> The band for the third well is good. WE GOT IT !!!!! <br> | ||
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| + | <center><img src="https://static.igem.org/mediawiki/2015/b/b8/Graph-180915.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br> | ||
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| + | <i class="fa fa-angle-right"></i> Induction of BBa_K325909 and BBa_K1159001 with a solution of arabinose 1%, and a solution of IPTG 1 mM for T7-Nanoluc (should correspond to the effect of a constitutive promoter like T7). Under these conditions, light intensity of T7-nanoluc was 1,2 time higher than the BBa_K1159001. <br> | ||
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| + | <center><img src="https://static.igem.org/mediawiki/2015/a/aa/Graph-180915_2.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br> | ||
| + | </p> | ||
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| + | <i class="fa fa-angle-right"></i> Effect of the concentration of IPTG inducer to the light intensity of T7-nanoluc part. While the concentration of IPTG does not exceed 1 mM, the light intensity remains low. <br> | ||
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| + | <center><img src="https://static.igem.org/mediawiki/2015/8/80/Graph-180915_3.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br> | ||
| + | </p> | ||
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| + | <i class="fa fa-angle-right"></i> Effect of the concentration of arabinose inducer to the light intensity of BBa_K1159001 part. Light intensity is linked to inducer concentration however over 1% of arabinose (final concentration), the intensity not rise anymore. | ||
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Latest revision as of 15:58, 18 September 2015
Results after PCR from 17/09
Expected results
Results
The band for the third well is good. WE GOT IT !!!!!
Induction of BBa_K325909 and BBa_K1159001 with a solution of arabinose 1%, and a solution of IPTG 1 mM for T7-Nanoluc (should correspond to the effect of a constitutive promoter like T7). Under these conditions, light intensity of T7-nanoluc was 1,2 time higher than the BBa_K1159001.
Effect of the concentration of IPTG inducer to the light intensity of T7-nanoluc part. While the concentration of IPTG does not exceed 1 mM, the light intensity remains low.
Effect of the concentration of arabinose inducer to the light intensity of BBa_K1159001 part. Light intensity is linked to inducer concentration however over 1% of arabinose (final concentration), the intensity not rise anymore.
18 Sept. 15