Difference between revisions of "Team:SDU-Denmark/Tour10"
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<img src="https://static.igem.org/mediawiki/2015/6/6a/SDU2015_Babymice.png" style="width:250px"/> | <img src="https://static.igem.org/mediawiki/2015/6/6a/SDU2015_Babymice.png" style="width:250px"/> | ||
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| − | <div class="thumbcaption"><i>Figure 1:</i> | + | <div class="thumbcaption"><i>Figure 1:</i> Photo of baby mice taken at the animal facilities at Odense University Hospital (OUH). </div> |
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| − | <a class="popupImg alignRight" style="width:250px" target="_blank" href="https://static.igem.org/mediawiki/2015/3/38/SDU2015_Gallery_WorkingintheLaboratort.png" title=" | + | <a class="popupImg alignRight" style="width:250px" target="_blank" href="https://static.igem.org/mediawiki/2015/3/38/SDU2015_Gallery_WorkingintheLaboratort.png" title="Team members working in the laboratory."> |
<img src="https://static.igem.org/mediawiki/2015/3/38/SDU2015_Gallery_WorkingintheLaboratort.png" style="width:250px"/> | <img src="https://static.igem.org/mediawiki/2015/3/38/SDU2015_Gallery_WorkingintheLaboratort.png" style="width:250px"/> | ||
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| − | <span class="intro">Monoclonal antibodies (mAbs) are extensively used | + | <span class="intro">Monoclonal antibodies (mAbs) are extensively used in scientific and fundamental medical research</span>. |
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| − | + | The nowadays production methods of mAbs are not only time-consuming and costly but also at the expense of animal lives. Antibodies have many applications in research, diagnostics, and show great promise in treatment of cancer. But the advancement is held back due to the expensive and time consuming production. Therefore we propose an alternative to antibodies; peptide aptamers – produced in <i>Escherichia coli</i> (<i>E. coli</i>). The variable loop of the peptide aptamer is generated through a random nucleotide library and held together by an optimized, enzymatic inactive version of human Thioredoxin (hTrx) used as a scaffold protein. We are using the bacterial two-hybrid system to generate and at the same to screen for functioning peptide aptamers that are able to bind our chosen target proteins (‘antigens’). In this model bacteria expressing a functioning peptide aptamer will express Red Fluorescent Protein (RFP), which enables us to detect and isolate them. To challenge the antibody tradition, we are also proposing a production model. | |
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Revision as of 16:29, 18 September 2015
"Change is the law of life and those who look only to the past or present are certain to miss the future." - John F. Kennedy
Project description
Monoclonal antibodies (mAbs) are extensively used in scientific and fundamental medical research.
The nowadays production methods of mAbs are not only time-consuming and costly but also at the expense of animal lives. Antibodies have many applications in research, diagnostics, and show great promise in treatment of cancer. But the advancement is held back due to the expensive and time consuming production. Therefore we propose an alternative to antibodies; peptide aptamers – produced in Escherichia coli (E. coli). The variable loop of the peptide aptamer is generated through a random nucleotide library and held together by an optimized, enzymatic inactive version of human Thioredoxin (hTrx) used as a scaffold protein. We are using the bacterial two-hybrid system to generate and at the same to screen for functioning peptide aptamers that are able to bind our chosen target proteins (‘antigens’). In this model bacteria expressing a functioning peptide aptamer will express Red Fluorescent Protein (RFP), which enables us to detect and isolate them. To challenge the antibody tradition, we are also proposing a production model.
Our team consists of nine students from five different educations; philosophy, chemical engineering, biochemistry and molecular biology, biomedicine and medicine. During this last 6 months we have laughed, cried, built friendships and overcome a lot of struggles together. In the end of the day it has just been a whole lot of fun! To guide and help us through the journey we have had our four supervisors. Go to the next page the team and dig deeper into the members of the SDU 2015 team.