Difference between revisions of "Template:Paris Bettencourt/riboflavinBanner"

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     <ul><li>Successfully cloned whole Vitamin B2 enzymatic pathway in coling strain of E.coli. </li>
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     <ul><li>Successfully made an <i>E. coli</i> strain overproducing vitamin B2 .</li>
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         <li>Successfully cloned RibD, RibT25, RibT48, synthetic promoters P48 into Biobrick backbones and therefore generated                            four new BioBricks.</li></ul>
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         <li>BioBrick submitted to the BioBrick Registry: BBa_K1678001, BBa_K1678002, BBa_K1678003, BBa_K1678004</li></ul>
  
 
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Revision as of 16:41, 18 September 2015

Background

Aims

Results

Lactobacillus plantarum, part of natural microbiota of idli fermentation, was chosen to apply metabolic engineering for production of Vitamin B2. Vitamin B2 pathway from Bacillus subtilis was codon optimized for Lactobacillus plantarum and two metabolic bottlenecks were overcame by synthetic promoters to have increased Vitamin B2 production.
  • Successfully made an E. coli strain overproducing vitamin B2 .
  • BioBrick submitted to the BioBrick Registry: BBa_K1678001, BBa_K1678002, BBa_K1678003, BBa_K1678004