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| | |protocol= none | | |protocol= none |
| | |protocolurl=none | | |protocolurl=none |
| − | |goal= extra integration of DNA in thrC locus | + | |goal= making a biofilm stronger bu knockout abrB |
| | |description=Testing TSB* and TSB2.0 | | |description=Testing TSB* and TSB2.0 |
| | |conclusion=TSB* and TSB 2.0 were tested | | |conclusion=TSB* and TSB 2.0 were tested |
| | }} | | }} |
Revision as of 17:06, 18 September 2015
Test difference between TSB* and TSB 2.0 (ab1)
Testing TSB* and TSB2.0
making a biofilm stronger bu knockout abrB
TSB* and TSB 2.0 were tested
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/none">none</a>
00:00, 15 Augustus 2015 - 00:00, 15 Augustus 2015
TSB medium was made using our own recipe. TSB* and TSB 2.0. TSB* is from Wikipedia and TSB 2.0 is based on literature, what amounts of glucose and NaCl are beneficial for biofilm growth. Test difference between TSB* and TSB 2.0 with With four different strains: JH642, PY79, 3610 comI and -3610 comI ΔabrB on 4 different carriers: nylon, W2, W4 and W6. Experiment was performed in triplo.
OD measurement
JH 0.85
PY 0.67
3610 1.53
ΔabrB 0.40
All ODs were diluted until 1.5 mL with an OD of 0.22 was obtained. The samples were diluted with water.
An OD of 0.22 was used to make comparing with the 128 plates experiment easier.