Difference between revisions of "Team:Warwick/Protocols"
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<br>Objective: To ensure that our zinc finger binding domains are able to bind to a glass slide, and can be visualised through immunofluorescent microscopy. | <br>Objective: To ensure that our zinc finger binding domains are able to bind to a glass slide, and can be visualised through immunofluorescent microscopy. | ||
<ul> | <ul> | ||
| − | + | <br><li>Glass slides were prepared (<ahref="https://static.igem.org/mediawiki/2015/f/f8/WarwickGlassSlideProtocol.pdf">Glass Slide Preperation Protocol</a>) by being cleaned and functionalised (with HCl and GOPTS respectively).</li> | |
<li>Specifically designed oligonucleotides containing zinc finger binding domains were introduced to the slides.</li> | <li>Specifically designed oligonucleotides containing zinc finger binding domains were introduced to the slides.</li> | ||
<li>These oligonucleotides comprise of a general adaptor strand, a specific short strand and a specific long strand.</li> | <li>These oligonucleotides comprise of a general adaptor strand, a specific short strand and a specific long strand.</li> | ||
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<ul> | <ul> | ||
<p>________________________________________________________________________________________________________________________________________________</p> | <p>________________________________________________________________________________________________________________________________________________</p> | ||
| − | <h3> Experiment 2: Testing the expression of zinc finger proteins | + | <h3> Experiment 2: Testing the expression of zinc finger proteins on the surface of E. coli cells upon induction with IPTG </h3> |
Objective: To ensure that our plasmid is being translated into protein, folding correctly and then being transported to the cell membrane effectively. Through the binding of fluorescent antibodies to the cell surface, we should be able to visualise the cells. | Objective: To ensure that our plasmid is being translated into protein, folding correctly and then being transported to the cell membrane effectively. Through the binding of fluorescent antibodies to the cell surface, we should be able to visualise the cells. | ||
<p style="float: right;"> <img src="https://static.igem.org/mediawiki/2015/3/3d/Warwick_diagram_of_redirecting_protein.jpeg" height="500px" width="500px" border="50px"></p> | <p style="float: right;"> <img src="https://static.igem.org/mediawiki/2015/3/3d/Warwick_diagram_of_redirecting_protein.jpeg" height="500px" width="500px" border="50px"></p> | ||
Latest revision as of 18:15, 18 September 2015
