Difference between revisions of "Template:Paris Bettencourt/riboflavinBanner"

 
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       <th class="noBottom"><h3>Results</h3></td>
 
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       <td class="noTop">Lactobacillus plantarum, part of natural microbiota of idli fermentation, was chosen to apply metabolic engineering for production of Vitamin B2.  
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       <td class="noTop">Some microorganisms (e.g. <i>B. subtilis</i>) naturally produce riboflavin in high amount.
 
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       <td class="noTop">Vitamin B2 pathway from Bacillus subtilis was codon optimized for Lactobacillus plantarum and two metabolic bottlenecks were overcame by synthetic promoters to have increased Vitamin B2 production.
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       <td class="noTop">Make Idli batter microorganisms produce high amount of riboflavin during fermentation to increase the vitamin B2 content of the batter.
 
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     <ul><li>Successfully cloned whole Vitamin B2 enzymatic pathway in coling strain of E.coli. </li>
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     <ul><li>Successfully cloned the full riboflavin biosynthesis pathway of <i>B. subtilis</i> in <i>E. coli</i> and made it over-produce vitamin B2 and proceeded to chromosomaly integrate the full pathway in <i>Lactococcus lactis</i></li>
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         <li>Successfully cloned RibD, RibT25, RibT48, synthetic promoters P48 into Biobrick backbones and therefore generated                            four new BioBricks.</li></ul>
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         <li>BioBrick submitted to the BioBrick Registry: <a href="http://parts.igem.org/Part:BBa_K1678001"> BBa_K1678001</a>, <a href="http://parts.igem.org/Part:BBa_K1678002"> BBa_K1678002</a>, <a href="http://parts.igem.org/Part:BBa_K1678003"> BBa_K1678003</a>, <a href="http://parts.igem.org/Part:BBa_K1678004"> BBa_K1678004</a></li></ul>
  
 
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Latest revision as of 18:38, 18 September 2015

Background

Aims

Results

Some microorganisms (e.g. B. subtilis) naturally produce riboflavin in high amount. Make Idli batter microorganisms produce high amount of riboflavin during fermentation to increase the vitamin B2 content of the batter.
  • Successfully cloned the full riboflavin biosynthesis pathway of B. subtilis in E. coli and made it over-produce vitamin B2 and proceeded to chromosomaly integrate the full pathway in Lactococcus lactis
  • BioBrick submitted to the BioBrick Registry: BBa_K1678001, BBa_K1678002, BBa_K1678003, BBa_K1678004