Difference between revisions of "Template:Heidelberg/safety/lab"

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<h3 class="basicheader"> GENERAL SAFETY AND GOOD PRACTICE</h3>
 
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The first step towards good lab safety are personal precautions such as wearing lab coast, gloves in regards to the handled matter and safety goggles. As we’ve been working RNA which is highly sensible to contaminations we cleaned a part of our lab using hydrogenperoxide. During this act of cleaning we wore special mouthgards so we don’t breath in any of the chemical. </p>
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Before starting with the wetlab work, Dr. Barbara DiVentura gave us a safety instruction in which she informed us about how to work in the lab and what was considered as good practice.
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We not only separated a RNAsefree part of the lab, but also one for running gels, as we would be handling chemicals like EtBr and <a href=” https://www.thermofisher.com/za/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html”>SYBR Safe</a> for staining our gels. We tended to use SYBR Safe, as it is proposed to be less toxic. Other dangerous chemicals were of course handled under the fume hood.  
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The first step towards good lab safety are personal precautions such as wearing lab coast, gloves in regards to the handled matter and safety goggles. As we have been working RNA which is highly sensible to contaminations, we cleaned a part of our lab using hydrogenperoxide. During this act of cleaning we wore special mouthgards so that we would not breathe in any of the potentially harmful chemicals.
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Not only did we separate a section for working but also one for programming and documentation, as we decided never to handle any electronics wearing gloves.
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We not only separated a RNAse free part of the lab, but also designated a special area for running gels, as we would be handling chemicals like EtBr and <a href="https://www.thermofisher.com/za/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html">SYBR Safe</a> for staining. We tended to use SYBR Safe, as it is proposed to be less toxic and as we wanted to keep the use of dangerous substances as low as possible. Other dangerous chemicals, such as beta-mercaptoethanol, TEMED, organic solvents and acrylamide were of course handled under the fume hood.
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Besides the different working sections, we also separated programming and documentation from bench work, as we decided never to handle any electronics wearing gloves. With regard to that, we were very lucky to have access to our own office two floors upstairs from our lab. There we had enough space for several computers and laptops, so that the programming could be safely separated from the lab work.
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As a precaution against spread of modified organisms, equipment and benches were regularly cleaned using EtOH. Also, we used designated trashcans for any living matter that were then autoclaved afterwards. We were highly sensible regarding possible spillages of E. coli as that might ruin our RNA based experiments, because of which we chose not to use certain parts of equipment for cells in order to keep them clean and our samples safe.
 
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Used organisms, namely E. coli strains DH5 \alpha and TOP10, yeast strain PY741 and
 
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As a precaution against spread of modified organisms equipment and benches were regularly cleaned using EtOH. Also, we used designated trashcans for any living matter that were then autoclaved afterwards. We were highly sensible regarding possible spillages of E. coli as that might ruin our RNA based experiments, because of which we chose not to use certain parts of equipment for cells.
 
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Latest revision as of 18:49, 18 September 2015

GENERAL SAFETY AND GOOD PRACTICE

Before starting with the wetlab work, Dr. Barbara DiVentura gave us a safety instruction in which she informed us about how to work in the lab and what was considered as good practice.

The first step towards good lab safety are personal precautions such as wearing lab coast, gloves in regards to the handled matter and safety goggles. As we have been working RNA which is highly sensible to contaminations, we cleaned a part of our lab using hydrogenperoxide. During this act of cleaning we wore special mouthgards so that we would not breathe in any of the potentially harmful chemicals.

We not only separated a RNAse free part of the lab, but also designated a special area for running gels, as we would be handling chemicals like EtBr and SYBR Safe for staining. We tended to use SYBR Safe, as it is proposed to be less toxic and as we wanted to keep the use of dangerous substances as low as possible. Other dangerous chemicals, such as beta-mercaptoethanol, TEMED, organic solvents and acrylamide were of course handled under the fume hood.

Besides the different working sections, we also separated programming and documentation from bench work, as we decided never to handle any electronics wearing gloves. With regard to that, we were very lucky to have access to our own office two floors upstairs from our lab. There we had enough space for several computers and laptops, so that the programming could be safely separated from the lab work.

As a precaution against spread of modified organisms, equipment and benches were regularly cleaned using EtOH. Also, we used designated trashcans for any living matter that were then autoclaved afterwards. We were highly sensible regarding possible spillages of E. coli as that might ruin our RNA based experiments, because of which we chose not to use certain parts of equipment for cells in order to keep them clean and our samples safe.