Difference between revisions of "NJU-China-safty.html"

 
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<ul style="margin-top:10px">
 
<li><a class="button" href="#detail" style="font-weight:bold;font-family:Microsoft YaHei">content</a></li>
 
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     <TR height="100%">
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<ul id="nav" style="height:100px;line-height:100px;margin-top:20%;margin-left:10%;list-style-type:none" class="dyn">
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</br>
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<li><a href="https://2015.igem.org/Team:NJU-China" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Home</a></li>
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<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Home</a></li>
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<li><a href="https://2015.igem.org/NJU-China-project.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Project</a></li>
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<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-background.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Background</a></li>
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<li><a href="https://2015.igem.org/NJU-China-notebook.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Notebook</a></li>
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<div style="line-height:250%;margin-left:10%" id="main1" onClick="document.all.child0.style.display=(document.all.child0.style.display =='none')?'':'none'" ><a href="#" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Project</a></div>
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<li><a href="#" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Model</a>
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<div id="child0" style="display:none">
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        <ul>  
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<ul>
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            <li><a href="https://2015.igem.org/NJU-China-model.html#deliverymodel" style="font-weight:bold;font-family:幼圆;font-size:20px;color:black">Delivery model</a></li>  
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<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China/Design" style="font-weight:bold;font-family:幼圆;color:black">design</a></li>
−
            <li><a href="#" style="font-weight:bold;font-family:幼圆;font-size:20px;color:black">RNAi model</a></li>  
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<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-project/result.html" style="font-weight:bold;font-family:幼圆;color:black">results</a></li>
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        </ul>  
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<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-project/conclusion.html" style="font-weight:bold;font-family:幼圆;color:black">conclusion</a></li>
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</li>
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<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-project/future work.html" style="font-weight:bold;font-family:幼圆;color:black">future work</a></li>
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<li style="line-height:250%"><a href="https://2015.igem.org/NJU-China-human-practice.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Human Practice</a></li>
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</ul>
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<li style="line-height:250%"><a href="https://2015.igem.org/NJU-China-safty.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Safty</a></li>
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</div>
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<li style="line-height:250%"><a href="https://2015.igem.org/NJU-China-team.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Team</a></li>
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<div style="line-height:250%;margin-left:10%" id="main1" onClick="document.all.child1.style.display=(document.all.child1.style.display =='none')?'':'none'" ><a href="#" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Model</a></div>
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<li style="line-height:250%"><a href="https://2015.igem.org/NJU-China-aknow.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Acknowledgement</a></li>
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<div id="child1" style="display:none">
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</ul>  
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<ul>
−
 
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<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-model.html" style="font-weight:bold;font-family:幼圆;color:black">Delivery Module</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China/RNAi" style="font-weight:bold;font-family:幼圆;color:black">RNAi Module</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China/signaling" style="font-weight:bold;font-family:幼圆;color:black">Signaling Module</a></li>
 +
</ul>
 +
</div>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China/Practices" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Human Practice</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-parts.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Parts</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-team.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Team</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-attribution.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Attribution</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China/Collaborations" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Collaborations</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-safty.html" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Safety</a></li>
 +
<div style="line-height:250%;margin-left:10%" id="main2" onClick="document.all.child2.style.display=(document.all.child2.style.display =='none')?'':'none'" > <a href="#" style="font-weight:bold;font-family:幼圆;font-size:25px;color:black">Notebook </div>
 +
<div id="child2" style="display:none">
 +
<ul>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-notebook.html#methods" style="font-weight:bold;font-family:幼圆;font-size:20px;color:black">Methods</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-notebook.html#protocal" style="font-weight:bold;font-family:幼圆;font-size:20px;color:black">Protocal</a></li>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-notebook.html#notebook" style="font-weight:bold;font-family:幼圆;font-size:20px;color:black">Notebook</a></li>
 +
</ul>
 +
</div>
 +
<li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-acknowledgement.html#notebook" style="font-weight:bold;font-family:幼圆;font-size:20px;color:black">Acknowledgement</a></li>
  
 
</TD>
 
</TD>
 
     <TD width="73%" bgColor=#FFFFFF style="vertical-align:top;padding-left:80px;padding-right:80px;padding-top:50px;padding-bottom:50px;word-wrap:break-word;">
 
     <TD width="73%" bgColor=#FFFFFF style="vertical-align:top;padding-left:80px;padding-right:80px;padding-top:50px;padding-bottom:50px;word-wrap:break-word;">
−
你在火中涅槃，灵魂不死；你在花间长眠，记忆不消。绚烂的阳光是迎接你西去的光环，不知你独自一人去那方可会感到孤单？
+
<h2>Specific Safety Concerns</h2>
−
[一]魂去
+
        <B>Chassis Organisms</B></br>
−
      起初听到你的死，我是愕然的。毕竟听说你的病情好了不少，却不料那只是最后的回光返照。
+
Our chassis is HEK293 cell line, from Homo sapiens. It is a very safe type of cell line wildly used in cell research. </br></br>
−
      我没有流泪，甚至没有悲伤，脑子里只是混混沌沌的一团。死？那是我们所有人最后的归宿吧。或许于你更是能够减少痛苦，未尝不是一件好事。
+
        <B>Laboratory Safety</B></br>
−
      一切被我想得太简单，甚至太过理所当然了。理智，真是一个可怕的局。
+
During the experiment, we regularly isolate exosomes from the culture medium of HEK293 cells. Then the exosomes are added to the culture medium of various types of cell lines such as A549, Neuro2A, C2C12 and MCF-7, after which we inject the exosomes into C57BL/6J mice that are commonly used in animal experiments. All of these cell lines are nontoxic and commonly used in scientific research. Our operations are strictly held in biosafety cabinets, and the animal experiments are under the control of our instructor. The ethical board of Nanjing University has approved the experimental procedure.</br></br>
−
[二]席间
+
During the seven months of experiment, we choose to use safe gene segments and reagents. All other reagents such as morphine are under strict control of the instructor. In our project, we use a peptide of RVG (rabies viral glycoprotein). Although sounds horrible, the part we use is actually a small sequence of a peptide, which holds none original biological activity and is therefore harmless to human.
−
      小号、大鼓敲个不停，那种声音不得不说是一种噪音。良久声止，开始一个个念悼词。
+
</br></br>
−
      我这时才发现，原来我并没有那么冷漠，缘是城市的喧嚣麻木了我的心。想起那年你的温言软语、慈爱笑容，泪竟是就这样忍不住地从眼眶中溢出了。我撇过头，不愿让人看见，嘴角仍是挂着笑容。
+
Generally, our project design has little chance to harm human bodies.</br></br>
−
      呵呵，我竟觉着在这葬礼上哭泣都是可耻的。望着周围人谈笑风生，我的心有点寒。到场的那么多人，究竟又有几个是真心的呢？可怜，还是可悲啊？
+
<h2>Training and Enforcement</h2>
−
      原来人可以铁石心肠这种程度，可毕竟他们中很多人比我和你的关系更近啊！听着那个花钱雇来哭的人呜咽，看着那人热泪纵横，我终是忍不住苦笑，竟是一个毫不相识的外人哭得最伤心。这是讽刺，还是笑话？
+
All team members participate in lab work and learn biosafety rules in our lab, such as how to use the equipments (e.g., biosafety cabinet). The team members also learn how to deal with the laboratory waste such as cell culture medium and sharp objects. Before entering laboratory, the team members systematically learn how to protect themselves if there is any accident such as gas leaking. During experiments we normally wear rubber gloves and lab coats for protection, and sterilize materials and wastes if necessary. Regarding animal experiments, the chosen team members all have received specific training before experiments and conduct experiments with the help of technicians in our lab.</br></br>
−
  [三]送葬
+
<h2>Future Implications</h2>
−
      你说，为什么他们还能那样淡然，可我偏偏忍不住泪流满面？为什么他们那个惊讶的表情让我竟是感到羞愧？好像自己做了什么惊天动地的事一样？
+
Currently we engineer siRNA-loaded exosomes to treat opiate/opioid addiction in animal model. In the future, we expect to isolate blood cells from patients with drug addiction, which can be engineered as cell factory to produce exosomes. Moreover, the exosomes can be engineered to express a RVG peptide (specifically recognize and target neuronal cells) on the exosomal membrane surface and filled with siRNA of the Mu opioid receptor (MOR, a primary target for opiates/opioids). Finally we will inject these exosomes that can efficiently pass blood-brain barrier and specifically accumulate in brain into bloodstream of patients to reduce MOR mRNA and protein levels and treat opiate/opioid addiction. This treatment may cause immune reactivity in human body and alteration of some gene in the brain if fully developed into a real product. To reduce the risks, we will strictly follow the general guidelines of scientific research and plan to conduct quantities of animal experiments. We firstly test the drug’s efficiency and safety in little animals such as mice. Only when the exosomes are guaranteed to be safe and ideally effective can we perform next step experiments in larger animals such as dogs. The further experiments will be performed in primates to ensure its safety. If accomplished, we can expect to realize it on human beings.
−
      执着一根香，撒下一碗酒，三拜三叩，泪融于酒，我如若痴人。
+
−
      照礼我是不必披麻戴孝的，只需戴着那顶白色的帽子即可。沿着幽僻的小路，我跟着吹着小号开路的队伍走在末尾。
+
−
      风景甚好，埋在那棵大树底下，你会不会成为那树的枝，树的叶？
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−
[四]感怀
+
−
      死生有命，无可更改。谋事在人，成事在天。任你生前浮华万千，到头来不过一抔尘土，一捧青灰。愿逝者安息，生者恬淡。
+
−
      有时候我们看不到自己拥有的美好，直到失去才恍然叹息。逝者已矣，页已掀过，惜福、感恩，生活其实温暖如花。
+
−
      花开半夏，倾尽繁华，愿你回眸半刹。你可听到我在同你夜话？
+
 
</TD>
 
</TD>
 
     </TR>
 
     </TR>

Latest revision as of 18:56, 18 September 2015

humanpractice


  • Home
  • Background
  • Human Practice
  • Parts
  • Team
  • Attribution
  • Collaborations
  • Safety
  • Acknowledgement
    		•

    Specific Safety Concerns

    Chassis Organisms
    Our chassis is HEK293 cell line, from Homo sapiens. It is a very safe type of cell line wildly used in cell research.

    Laboratory Safety
    During the experiment, we regularly isolate exosomes from the culture medium of HEK293 cells. Then the exosomes are added to the culture medium of various types of cell lines such as A549, Neuro2A, C2C12 and MCF-7, after which we inject the exosomes into C57BL/6J mice that are commonly used in animal experiments. All of these cell lines are nontoxic and commonly used in scientific research. Our operations are strictly held in biosafety cabinets, and the animal experiments are under the control of our instructor. The ethical board of Nanjing University has approved the experimental procedure.

    During the seven months of experiment, we choose to use safe gene segments and reagents. All other reagents such as morphine are under strict control of the instructor. In our project, we use a peptide of RVG (rabies viral glycoprotein). Although sounds horrible, the part we use is actually a small sequence of a peptide, which holds none original biological activity and is therefore harmless to human.

    Generally, our project design has little chance to harm human bodies.

    Training and Enforcement

    All team members participate in lab work and learn biosafety rules in our lab, such as how to use the equipments (e.g., biosafety cabinet). The team members also learn how to deal with the laboratory waste such as cell culture medium and sharp objects. Before entering laboratory, the team members systematically learn how to protect themselves if there is any accident such as gas leaking. During experiments we normally wear rubber gloves and lab coats for protection, and sterilize materials and wastes if necessary. Regarding animal experiments, the chosen team members all have received specific training before experiments and conduct experiments with the help of technicians in our lab.

    Future Implications

    Currently we engineer siRNA-loaded exosomes to treat opiate/opioid addiction in animal model. In the future, we expect to isolate blood cells from patients with drug addiction, which can be engineered as cell factory to produce exosomes. Moreover, the exosomes can be engineered to express a RVG peptide (specifically recognize and target neuronal cells) on the exosomal membrane surface and filled with siRNA of the Mu opioid receptor (MOR, a primary target for opiates/opioids). Finally we will inject these exosomes that can efficiently pass blood-brain barrier and specifically accumulate in brain into bloodstream of patients to reduce MOR mRNA and protein levels and treat opiate/opioid addiction. This treatment may cause immune reactivity in human body and alteration of some gene in the brain if fully developed into a real product. To reduce the risks, we will strictly follow the general guidelines of scientific research and plan to conduct quantities of animal experiments. We firstly test the drug’s efficiency and safety in little animals such as mice. Only when the exosomes are guaranteed to be safe and ideally effective can we perform next step experiments in larger animals such as dogs. The further experiments will be performed in primates to ensure its safety. If accomplished, we can expect to realize it on human beings.