Difference between revisions of "Team:Warwick/Description"

 
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<h2> Project Description </h2>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h4>Advice on writing your Project Description</h4>
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<h4>Characterisation of An Existing Part</h4>
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<p>As our project had a large focus on fluorescence, we decided to characterise a part from the existing iGEM registry that produces red fluorescent protein in order to determine whether we could use it in our project. We decided against using RFP, however here are the results and methods used in the characterisation.</p>
  
 
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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<ul> <li> The J04450 gene from the registry was cloned into three different plasmids (pSB1K3, pSB3K3, and pSB4K4) each with a different copy number. </li>
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<li>Each plasmid was transformed into electrocompetent MG1655-Z1</li>
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<li>Three colonies of each plasmid were then inoculated in LB and grown overnight.</li>
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<li>In the morning, each inoculation was refreshed in M9 + casein amino acid (500x dilution), induced with different concentrations of IPTG (0uM, 250uM, and 500uM IPTG) and grown for another 6 hours.</li>
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<li> They were then transferred into a 96 well plate (200uL per well) and measured their fluorescence every 15 minutes.</li>
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<p>The results for our characterisation can be found on the <a href="http://parts.igem.org/Part:BBa_J04450">J04450 part page in the registry</a>.
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h4>References</h4>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
 
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<h4>Inspiration</h4>
 
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<p>See how other teams have described and presented their projects: </p>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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Latest revision as of 19:15, 18 September 2015

Warwick iGEM 2015

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