Difference between revisions of "Team:KU Leuven/Research/Basic Part"
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+ | <h2>CheZ-GFP</h2> | ||
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+ | <h2>LuxI-His</h2> | ||
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+ | <div id="toggletwo" > | ||
+ | <p><b>Theory</b><br/> | ||
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+ | <h2>Motility Test Assay</h2> | ||
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+ | <p><b>Protocol</b></p> | ||
+ | <dl> | ||
+ | <dd>1. Prepare selective media (LB with 0.25% agar (2,5 g/L) in Petri dishes (85 mm dia).</dd> | ||
+ | <dd>2. Apply 1.5 µL of the diluted cell suspensions from mid-log-phase cultures (~2×105 cells/µL (OD=0.5)) to the center of the plates, and let dry in air for 15 min. </dd> | ||
+ | <dd>3. Incubate at 37 °C for 10 h. </dd> | ||
+ | </dl> | ||
</div> | </div> |
Revision as of 19:37, 18 September 2015
Basics Parts
On this page you can find all our basic parts needed for the system to work. To learn more about them, click the titles below!
Name | Construct | Length | Type |
---|---|---|---|
BBa_K1709002 | CheZ-GFP | 1401 bp | Coding |
BBa_K1709000 | LuxI-His | 609 bp | Coding |
BBa_K1709004 | LuxR-E | 835 bp | Coding | BBa_K1709005 | Ag43-YFP | 3843 bp | Coding |
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be