Difference between revisions of "Team:NJU-China"

 
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<B>Opioids and opiates top the list of illicit drugs and cause the most burden of disease and drug-related deaths worldwide. The goal of this project is to develop a strategy to treat opioid addiction. We prepared exosomes (nano-sized vesicles secreted by endogenous cells) from cell factory that was engineered to express a rabies viral glycoprotein peptide (specifically recognize and target neuronal cells) on the exosomal membrane surface. By filling the exosomes with siRNA of the mu opioid receptor (MOR, a primary target for opioids) and injecting the exosomes into mouse bloodstream, we detected efficient passage of the siRNA through blood-brain barrier and specific accumulation of the siRNA in mouse brain. Consequently, siRNA-loaded exosomes significantly reduced MOR mRNA and protein levels in mice. To show the therapeutic potential, siRNA-loaded exosomes strongly restrained morphine-induced conditioned place preference in mice. This project may open up new avenues for future treatment of drug addiction.</B>
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<div id="menu">
 
<div id="menu">
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<table style="width:100%;margin-top:500px">
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<table style="width:100%;margin-top:50px">
 
<tr>
 
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Latest revision as of 19:46, 18 September 2015

humanpractice