Difference between revisions of "Team:Chalmers-Gothenburg/Protocols"
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<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| − | <p><b>PCR:</b> https://2015.igem.org/File:ChalmersGothenburgPCRprotocol.pdf</p> | + | <p><b>PCR:</b> https://2015.igem.org/File:ChalmersGothenburgPCRprotocol.pdf </p> |
<p><b>PCR Purification:</b></p> | <p><b>PCR Purification:</b></p> | ||
Revision as of 19:49, 18 September 2015
Protocols
PCR: https://2015.igem.org/File:ChalmersGothenburgPCRprotocol.pdf
PCR Purification:
Gel extraction:
Gibson Assembly Method:
Sticky-end DNA Insert Ligation into Vector DNA:
FastDigest Restriction:
Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation:
Yeast Genomic DNA Extraction LiOAc-SDS:
Yeast Transformation by Electroporation:
Test of Detection System in Saccharomyces cerevisiae: