Difference between revisions of "Team:Czech Republic/Description"

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<h2> Project Description </h2>
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= pGAL1 ([http://parts.igem.org/Part:BBa_J63006 BBa_J63006]) =
  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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[[File:Czech_Republic_pgal_timeresponse.png|500px|left]]
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<br />
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<h5>What should this page contain?</h5>
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[[File:Czech_Republic_pgal_timeactivity.png|500px|left]]
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<ul>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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</ul>
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[[File:Czech_Republic_pgal_activity.png|500px|left]]
  
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<br />
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3 different concentrations (0.5%, 1%, 2%) of galactose were added to 100 µl yeast culture in SD min medium. We measured the fluorescence in 20 minutes intervals for 6 hours using a plate reader.
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<h4>Advice on writing your Project Description</h4>
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<p>
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The first graph shows evolution of fluorescence in time. We subtracted fluorescence of negative control from the fluorescence of each sample and divided this by difference between sample´s and negative control´s ODs in order to obtain RFU units.
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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</p>
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<p>
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Our measurement showed that the higher concentrations causes quicker and higher activation of the promoter.  
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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</p>
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Since 2%  appears to be the convenient concentration for maximal activation of the promoter in our experiment we used it as a reference and related the activity of other concentration to this concentration. Results are displayed in the second graph.
  
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<br />
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The third graph show the static response characteristic, dependence of activation of concentration, related to the 2% concentration.
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<h4>References</h4>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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<html><div style="clear: both;"></div></html>
  
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= pCUP1 ([http://parts.igem.org/Part:BBa_K945002 BBa_K945002]) =
  
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<h4>Inspiration</h4>
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The activity of pCUP1 promoter was characterized by transforming pCUP1-GFP plasmids into yeast S. cerevisiae and measuring the evolution of fluorescence in time.
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<p>See how other teams have described and presented their projects: </p>
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[[File:Czech_Republic_CUP1.png|500px|left|border]]
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5 different concentrations (50 μM, 100 μM, 300 μM, 500 μM and 800 μM) of CUP were added to 100 µl yeast culture in SD min medium. 100 μM concentration was recommended as the most efficient in literature. We measured the fluorescence in 20 minutes intervals for 140 minutes using a plate reader.
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The first graph shows evolution of fluorescence in time. We subtracted fluorescence of negative control from the fluorescence of each sample and divided this by difference between sample´s and negative control´s ODs in order to obtain RFU units.
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Our measurement confirmed that 100 μM concentration causes quickest activation of the pCUP promoter. The obtained curve is almost linear. 300 μM concentration curve is similar, however the maximal activation of the promoter is slightly lower. Activation when using 500 μM concentration is relatively low. Measurement showed that the 800 μM is fatal for the yeast culture.
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[[File:Czech_Republic_CUP2.png|500px|left]]
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Since 100 μM appears to be the convenient concentration for maximal activation of the promoter we used it as a reference and related the activity of other concentration to this concentration. Results are displayed in the second graph.
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<ul>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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</ul>
 
  
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</div>
 
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{{:Team:Czech_Republic/Template:Bottom}}

Latest revision as of 19:55, 18 September 2015

Description

pGAL1 ([http://parts.igem.org/Part:BBa_J63006 BBa_J63006])

Czech Republic pgal timeresponse.png
Czech Republic pgal timeactivity.png
Czech Republic pgal activity.png

3 different concentrations (0.5%, 1%, 2%) of galactose were added to 100 µl yeast culture in SD min medium. We measured the fluorescence in 20 minutes intervals for 6 hours using a plate reader.

The first graph shows evolution of fluorescence in time. We subtracted fluorescence of negative control from the fluorescence of each sample and divided this by difference between sample´s and negative control´s ODs in order to obtain RFU units.

Our measurement showed that the higher concentrations causes quicker and higher activation of the promoter.

Since 2% appears to be the convenient concentration for maximal activation of the promoter in our experiment we used it as a reference and related the activity of other concentration to this concentration. Results are displayed in the second graph.

The third graph show the static response characteristic, dependence of activation of concentration, related to the 2% concentration.

pCUP1 ([http://parts.igem.org/Part:BBa_K945002 BBa_K945002])

The activity of pCUP1 promoter was characterized by transforming pCUP1-GFP plasmids into yeast S. cerevisiae and measuring the evolution of fluorescence in time.

Czech Republic CUP1.png

5 different concentrations (50 μM, 100 μM, 300 μM, 500 μM and 800 μM) of CUP were added to 100 µl yeast culture in SD min medium. 100 μM concentration was recommended as the most efficient in literature. We measured the fluorescence in 20 minutes intervals for 140 minutes using a plate reader.

The first graph shows evolution of fluorescence in time. We subtracted fluorescence of negative control from the fluorescence of each sample and divided this by difference between sample´s and negative control´s ODs in order to obtain RFU units.

Our measurement confirmed that 100 μM concentration causes quickest activation of the pCUP promoter. The obtained curve is almost linear. 300 μM concentration curve is similar, however the maximal activation of the promoter is slightly lower. Activation when using 500 μM concentration is relatively low. Measurement showed that the 800 μM is fatal for the yeast culture.

Czech Republic CUP2.png

Since 100 μM appears to be the convenient concentration for maximal activation of the promoter we used it as a reference and related the activity of other concentration to this concentration. Results are displayed in the second graph.