Difference between revisions of "Team:Gifu/result-page"
(4 intermediate revisions by the same user not shown) | |||
Line 170: | Line 170: | ||
</font> | </font> | ||
− | <br><br><br><br> | + | <br><br><br><br><blockquote><blockquote> |
<font size="4" face="Century"><b> FUNCTION </b></font> | <font size="4" face="Century"><b> FUNCTION </b></font> | ||
<font size="3"face="Arimo"> | <font size="3"face="Arimo"> | ||
Line 193: | Line 193: | ||
There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function. | There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function. | ||
</br> | </br> | ||
− | + | <br><br></blockquote></blockquote> | |
<p> | <p> | ||
− | <div class="center">Table 4. The generators used to research the qualitative of a protein | + | <div class="center">Table 4. The generators used to research the qualitative of a protein</div><br> |
<table class="partsjump" align="center"> | <table class="partsjump" align="center"> | ||
<tr> | <tr> | ||
Line 241: | Line 241: | ||
− | + | <blockquote><blockquote> | |
<br clear="all"><br> | <br clear="all"><br> | ||
− | <font size="4"face="Arimo"><li>Reason of why | + | <font size="4"face="Arimo"><li><b>Reason of why |
<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1859020" > [BBa_K1859020] </a> | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1859020" > [BBa_K1859020] </a> | ||
and | and | ||
<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1859022" > [BBa_K1859022] </a> | <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K1859022" > [BBa_K1859022] </a> | ||
− | don’t synthesize the long chain protein</li><br> | + | don’t synthesize the long chain protein</b></li><br> |
</font> | </font> | ||
In this experiment, | In this experiment, | ||
Line 257: | Line 257: | ||
We made prediction of synthesized mRNA’s secondary structure. | We made prediction of synthesized mRNA’s secondary structure. | ||
</br> | </br> | ||
+ | </blockquote></blockquote> | ||
+ | |||
<img src="https://static.igem.org/mediawiki/2015/d/d3/Gifu-%E7%92%B0%E7%8A%B62014.png" width="320" height="201" vspace="10" hspace="20" align="left"></img> | <img src="https://static.igem.org/mediawiki/2015/d/d3/Gifu-%E7%92%B0%E7%8A%B62014.png" width="320" height="201" vspace="10" hspace="20" align="left"></img> | ||
Line 334: | Line 336: | ||
</p> | </p> | ||
<p> | <p> | ||
+ | |||
+ | <blockquote><blockquote> | ||
We can see the mRNA which is not to synthesize a long chain protein (BBa_K1859020 and BBa_K1859022) forms hydrogen bonds in downstream of the ribosome binding site. | We can see the mRNA which is not to synthesize a long chain protein (BBa_K1859020 and BBa_K1859022) forms hydrogen bonds in downstream of the ribosome binding site. | ||
It is assumed that these binds prevent mRNA from starting to be translated.<br><br><br> | It is assumed that these binds prevent mRNA from starting to be translated.<br><br><br> | ||
</p> | </p> | ||
− | + | </blockquote></blockquote> | |
Latest revision as of 20:08, 18 September 2015
normal | outside | insideⅠ | insideⅡ | |
---|---|---|---|---|
Efficiency of circularization (relativity value) | 1.00 | 2.05 | 1.22 | 1.34 |
FUNCTIONWe made 7 kinds of linker in this experiment.
We made parts which have these sequence of linker in the downstream of the 3’ side of the intron [BBa_K1332005] or the upstream of the 5’ side of the intron without stop codon [BBa_K1332003].
We constructed plasmids like following it.In case of inserting these plasmid into E. coli, the following circular mRNA is expressed and the long chain protein is synthesized.
We inserted these plasmid into an E. coli and made it synthesize proteins and did SDS-PAGE using this proteins. If the protein is not boiled, we can do SDS-PAGE keeping it fluorescence because RFP’s structure is strong. Therefore we applied samples that were not boiled.
![]()
![]()
Fig.4 Fluorescence of protein before dying by CBB Fig.5 after dying by CBB
![]()
Fig.6 Overlapping Fig.4 and Fig.5
There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.
No. | generator |
---|---|
A | R0010+ B0034+ K1332001+ B0015 |
B | BBa_K1332011 |
C | BBa_K1859027 |
D | BBa_K1859028 |
E | BBa_K1859020 |
F | BBa_K1859021 |
G | BBa_K1859022 |
H | BBa_K1859023 |
I | BBa_K1859023 |
J | BBa_K1859025 |
K | BBa_K1859024 |
Reason of why [BBa_K1859020] and [BBa_K1859022] don’t synthesize the long chain protein
In this experiment, [BBa_K1859020] and [BBa_K1859022] didn’t generate the long chain protein. The reason of this phenomenon can be deduced in terms of the sequence of the circular mRNA.
We made prediction of synthesized mRNA’s secondary structure.
![](https://static.igem.org/mediawiki/2015/d/d3/Gifu-%E7%92%B0%E7%8A%B62014.png)
![](https://static.igem.org/mediawiki/2015/6/6b/Gifu-%E3%81%A1%EF%BC%92.png)
![](https://static.igem.org/mediawiki/2015/3/3f/Gifu-%E3%81%A8%EF%BC%92.png)
![](https://static.igem.org/mediawiki/2015/9/98/Gifu-%E3%81%AB%E3%81%84.png)
![](https://static.igem.org/mediawiki/2015/7/7e/Gifu-%E3%81%AC%EF%BC%92.png)
![](https://static.igem.org/mediawiki/2015/a/a8/Gifu-%E3%81%BB%E3%82%8D.png)
![](https://static.igem.org/mediawiki/2015/9/92/Gifu-%E3%81%AB%E3%81%AF.png)
![](https://static.igem.org/mediawiki/2015/4/43/Gifu-%E3%82%8A%EF%BC%92.png)
![](https://static.igem.org/mediawiki/2015/5/58/Gifu-%E3%81%AF%E3%81%B8.png)
![](https://static.igem.org/mediawiki/2015/b/bc/Gifu-%E3%81%B8%E3%81%84.png)
We can see the mRNA which is not to synthesize a long chain protein (BBa_K1859020 and BBa_K1859022) forms hydrogen bonds in downstream of the ribosome binding site. It is assumed that these binds prevent mRNA from starting to be translated.