Difference between revisions of "Team:LZU-China/Parts"
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Revision as of 20:14, 18 September 2015
RibB is our basic gene. Every part basic on it.
Figure 1:Constitutive parts
K1755005 K1755006 K1755007 K1755008 and K1755009
Figure 2:Our parts working in MFC
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB). This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis.
Figure 3:Concentration of riboflavin vs Cu ions
Figure 4:Riboflavin concentration when the CuSO4 in 5mg/L
We construct many parts as the following list.
Parts list |
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|---|---|---|
| Part ID | Characterization | Details |
| K1755001 | ribB coding sequence | more information |
| K1755002 | ribB + B0014(terminator) | more information |
| K1755003 | B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator) | more information |
| K1755004 | K525998(PT7+RBS) + ribB + B0014(terminator) | more information |
| K1755005 | K608003(strong promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755006 | K608006(medium promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755007 | B608002(strong promoter+strong RBS)+ ribB + B0014(terminator) | more information |
| K1755008 | K608004(strong Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755009 | K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755301 | MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755302 | K190024(Copper sensor) + ribB + B0014(terminator) | more information |
| K1755303 | K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755305 | K346002(MerO) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755024 | K911003+ B0032(RBS) + ribB + B0014(terminator) | more information |