Difference between revisions of "Team:Tuebingen/Notebook"
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Ligation of gene blocks (Cre, Cre_sn, Dronpa, HCV, HCV+linker, nluc), linker L6, L9, L12, L15, nDegron and NLS with pSB1C3 and transformation in DH10beta: </p> | Ligation of gene blocks (Cre, Cre_sn, Dronpa, HCV, HCV+linker, nluc), linker L6, L9, L12, L15, nDegron and NLS with pSB1C3 and transformation in DH10beta: </p> | ||
Revision as of 20:17, 18 September 2015
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Dronpa-Caged Cre
Ligation of gene blocks (Cre, Cre_sn, Dronpa, HCV, HCV+linker, nluc), linker L6, L9, L12, L15, nDegron and NLS with pSB1C3 and transformation in DH10beta:
pSB-Cre_sn and pSB-Luciferase did not yield correct colonies (transformation repeated), the other constructs resulted in correct colonies
Cre Reporter Cassette
Ligation of gene blocks (tADH, loxP WT, loxP66, loxP71) with pSB1C3 and transformation in DH10beta
pRS Vectors
Mutagenesis primer design pRS313, pRS315, pRS316