Difference between revisions of "Team:WashU StLouis"
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− | - | + | -Take the bottle to an autoclave |
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− | - | + | -Autoclave bottle on agar cycle, loose cap, for two hours |
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− | (NOTE: | + | (NOTE: % glycerol in each bottle is roughly 4.4%) |
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− | -Bottles were placed back into centrifuge for another | + | -Bottles were placed back into centrifuge for another 25 minutes at 4 deg C |
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− | - | + | -Obtain plasmid DNA |
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− | - | + | -Pipet entirety of electrocompetent cell mixture into electro cuvette |
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− | - | + | -Shock cell/DNA mixture using an Eporator |
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− | - | + | -Take out strain and pour into 100 uL of LB to be grown for 1 hour |
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− | -After 1 hour of growth, strain | + | -After 1 hour of growth, plate strain onto LB/Agar/Antibiotic |
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− | - | + | -Mix 500 uL of cell culture with 500 uL of 30% glycerol |
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− | - | + | -Store in -80 deg C freezer |
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Revision as of 20:45, 18 September 2015
Project Overview
Washington University and Penn State iGEM
Team
NitroGeniuses
-
Jessica O'Callaghan
Chemical Engineering
Penn State
-
Laura Beebe
Biochemistry and Molecular Biology
Penn State
-
Blake Actkinson
Biomedical Engineering
Washington University in St. Louis
-
Charlotte Bourg
Biochemistry and Spanish
Washington University in St. Louis
-
David Ayeke
Computer Engineering
Washington University in St. Louis
-
Mike Toomey
Biomedical Engineering
Washington University in St. Louis
-
Caroline Focht
- 2014 iGEM Team MemberChemistry and Biochemistry
Washington University in St. Louis
Collaboration
Looking for Collaborators?
Vanderbilt
Our team worked closely with Vanderbilt University’s iGEM team over the summer to help them troubleshoot issues they had been having regarding a promoter and to come up with questions to ask our panel. Read more here
Help Us Out?
Washington University in St. Louis genetic engineering panel
Hey iGEM teams!
Here at Wash U we’re planning a panel on genetic engineering to be held in the fall for the Wash U community. We’re hoping to address some gray areas of synthetic biology and genetic engineering in agriculture, from safety and regulation to ethics and implications. We’re still finalizing our panelists, but hope to have experts from Monsanto and the Danforth Plant Science Center. We’re also in touch with some Wash U professors from the biology, philosophy, and anthropology departments.
We plan to start off the discussion with our own questions before opening it up to the audience. We’ve come up with a list of questions, but would love to get the input of some other iGEM teams. If you have questions you think would start interesting discussion at the panel, we’d love to hear them! Email us at
Our Amazing P.I's
The people that made it all possible
![](https://static.igem.org/mediawiki/2015/2/28/Moon_72.jpg)
Tae Seok Moon
Faculty Advisor - Washington University
![](https://static.igem.org/mediawiki/2015/3/3f/FZhang.png)
Fuzhong Zhang
Faculty Advisor - Washington University
![](https://static.igem.org/mediawiki/2015/4/4b/Cmaranas.jpg)
Costas Maranas
Faculty Advisor - Pennsylvania State University
![](https://static.igem.org/mediawiki/2015/0/0b/Cheryl_72.jpg)
Cheryl Immethun
Washington University iGEM Advisor
![](https://static.igem.org/mediawiki/2015/f/f2/Thomas.jpg)
Thomas Mueller
Penn State iGEM Advisor
![](https://static.igem.org/mediawiki/2015/c/cb/Andi.jpg)
Andrea Balassy
Washington University iGEM Advisor
![](https://static.igem.org/mediawiki/2015/f/fe/Carlos.png)
Carlos Barba
Washington University iGEM Advisor
![](https://static.igem.org/mediawiki/2015/b/b3/YiXiao.png)
Yi Xiao
Washington University iGEM Advisor
![](https://static.igem.org/mediawiki/2015/d/d0/Ray.jpg)
Ray Henson
Washington University iGEM Advisor
![](https://static.igem.org/mediawiki/2015/9/9c/Ryan_photo_small.jpg)
Young Je Lee
Washington University iGEM Advisor