Difference between revisions of "Team:Groningen/Notebook/Psalt GFP Validation pz81"
HarmRuesink (Talk | contribs) (Created page with "{{Template:Team:Groningen/TEMPLATES/BASE|pagename=LOGBOOK/Psalt_GFP_Validation_pz81}}") |
(No difference)
|
Latest revision as of 21:04, 18 September 2015
Blue Bio Energy
Psalt GFP Validation (pz81)
The transformed B. subtilis was tested to check if the salt inducible promotor works by adding different salt concentrations.
To create a salt inducible promotor.
No fluorescent protein visible.
None
00:00, 1 September 2015 - 00:00, 1 September 2015
This was done by growing B. subtilis ComI in LB + chloramphenicol for four hours.
Then different salt concentrations were added (0.1M, 0.5M and 1M).
This was incubated for 1 hour at 37°C. Afterwards cells were fixated in 1,25% agarose in PBS. Fluorescent microscopy did not show any differences between salt concentrations.