Latest revision as of 21:10, 18 September 2015

Tuesday 7th July

Labwork

Verification of our plasmids

by Coralie, Johan, Seong Koo, Audrey, Pauline

Biobricks verification

K115017
C0040
I13602
J23101
J23117
J23106
I13504

Extraction

With the Nucleospin Kit from Macherey Nagel

Digestion by NotI

Stock solution preparation :

124µL H2O
16µL buffer FastDigest 10x
4µL NotI

We use 18µL from this solution with 2µL plasmid solution

Incubation 1h30 at 37°C

Electrophoresis

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

ParisSaclay 070715-digestion vérif plasmides.jpg

Verification, from left to right, 1. DNA Ladder, 2. BBa_J23117, 3. BBa_J23101, 4. BBa_I13602, 5. BBa_I13504, 6. BBa_K115017, 7. BBa_C0040, 8. BBa_J23106, 9. Indicator, 10. Empty, 11. Empty, 12. Empty

New culture

by Pauline, Audrey

New liquid culture of:

K1493504

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Members present:

Instructors and advisors: Alice.
Students: Johan, Seong Koo, Audrey, Coralie, Pauline

Back to the calendar

@@ Line 1: / Line 1: @@
+{{Team:Paris_Saclay/notebook_header}}
 =Tuesday 7th July=
-==Back in Lab==
+==Labwork==
-====Verification of our plasmid====
+===Verification of our plasmids===
-''by Johan''
+''by Coralie, Johan, Seong Koo, Audrey, Pauline''
-Biobricks verification: K115017; C0040; I13602; J23101; J23117; J23106; I13504; K1493504
+====Biobricks verification====
-''by Coralie, Johan, Seon Koo, Audrey, Pauline''
+*K115017
+*C0040
+*I13602
+*J23101
+*J23117
+*J23106
+*I13504
+====Extraction====
+With the Nucleospin Kit from Macherey Nagel
+====Digestion by NotI====
+Stock solution preparation :
+* 124µL H2O
+* 16µL buffer FastDigest 10x
+* 4µL NotI
+We use 18µL from this solution with 2µL plasmid solution
+Incubation 1h30 at 37°C
 ====Electrophoresis====
-''by Johan, Seon Koo''
+''by Johan, Audrey, Pauline''
-====New culture====
+Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET
+Migration 0,06A 80V
+[[File:ParisSaclay_070715-digestion_vérif_plasmides.jpg|300px|center]]
+<html>
+<p><i>Verification, from left to right, 1. <a href="https://2015.igem.org/File:Paris_Saclay-Ladder.jpg" target="_blank">DNA Ladder</a>, 2. BBa_J23117, 3. BBa_J23101, 4. BBa_I13602, 5. BBa_I13504, 6. BBa_K115017, 7. BBa_C0040, 8. BBa_J23106, 9. Indicator, 10. Empty, 11. Empty, 12. Empty</i></p></html>
+===New culture===
 ''by Pauline, Audrey''
-New liquid cultures of:
+New liquid culture of:
 *K1493504
-x 5ml LB + 10μl Chloramphenicol + 1 bacteria colonies. We incubate cultures at 37°C.
+x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.
+We incubate cultures at 37°C, ON.
 '''Members present:'''
 * Instructors and advisors: Alice.
-* Students: Johan, Seon-Koo, Audrey, Coralie, Pauline
+* Students: Johan, Seong Koo, Audrey, Coralie, Pauline
+{{Team:Paris_Saclay/notebook_footer}}

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