Difference between revisions of "Team:Paris Saclay/Notebook/July/7"
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| + | {{Team:Paris_Saclay/notebook_header}} | ||
=Tuesday 7th July= | =Tuesday 7th July= | ||
| − | == | + | ==Labwork== |
| − | + | ===Verification of our plasmids=== | |
''by Coralie, Johan, Seong Koo, Audrey, Pauline'' | ''by Coralie, Johan, Seong Koo, Audrey, Pauline'' | ||
| − | + | ====Biobricks verification==== | |
| − | + | ||
*K115017 | *K115017 | ||
*C0040 | *C0040 | ||
| Line 13: | Line 13: | ||
*J23106 | *J23106 | ||
*I13504 | *I13504 | ||
| − | |||
| − | Extraction | + | ====Extraction==== |
| + | With the Nucleospin Kit from Macherey Nagel | ||
| − | + | ====Digestion by NotI==== | |
| + | Stock solution preparation : | ||
| + | * 124µL H2O | ||
| + | * 16µL buffer FastDigest 10x | ||
| + | * 4µL NotI | ||
| − | + | We use 18µL from this solution with 2µL plasmid solution | |
| − | + | Incubation 1h30 at 37°C | |
| − | + | ||
| − | + | ||
====Electrophoresis==== | ====Electrophoresis==== | ||
| Line 30: | Line 32: | ||
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET | Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET | ||
Migration 0,06A 80V | Migration 0,06A 80V | ||
| + | [[File:ParisSaclay_070715-digestion_vérif_plasmides.jpg|300px|center]] | ||
| + | <html> | ||
| + | <p><i>Verification, from left to right, 1. <a href="https://2015.igem.org/File:Paris_Saclay-Ladder.jpg" target="_blank">DNA Ladder</a>, 2. BBa_J23117, 3. BBa_J23101, 4. BBa_I13602, 5. BBa_I13504, 6. BBa_K115017, 7. BBa_C0040, 8. BBa_J23106, 9. Indicator, 10. Empty, 11. Empty, 12. Empty</i></p></html> | ||
| − | + | ===New culture=== | |
| − | + | ||
''by Pauline, Audrey'' | ''by Pauline, Audrey'' | ||
| − | New liquid | + | New liquid culture of: |
*K1493504 | *K1493504 | ||
| − | 2x 5ml LB + 10μl Chloramphenicol + 1 | + | 2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. |
| − | + | We incubate cultures at 37°C, ON. | |
'''Members present:''' | '''Members present:''' | ||
* Instructors and advisors: Alice. | * Instructors and advisors: Alice. | ||
* Students: Johan, Seong Koo, Audrey, Coralie, Pauline | * Students: Johan, Seong Koo, Audrey, Coralie, Pauline | ||
| + | |||
| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 21:10, 18 September 2015
Contents
Tuesday 7th July
Labwork
Verification of our plasmids
by Coralie, Johan, Seong Koo, Audrey, Pauline
Biobricks verification
- K115017
- C0040
- I13602
- J23101
- J23117
- J23106
- I13504
Extraction
With the Nucleospin Kit from Macherey Nagel
Digestion by NotI
Stock solution preparation :
- 124µL H2O
- 16µL buffer FastDigest 10x
- 4µL NotI
We use 18µL from this solution with 2µL plasmid solution
Incubation 1h30 at 37°C
Electrophoresis
by Johan, Audrey, Pauline
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V
Verification, from left to right, 1. DNA Ladder, 2. BBa_J23117, 3. BBa_J23101, 4. BBa_I13602, 5. BBa_I13504, 6. BBa_K115017, 7. BBa_C0040, 8. BBa_J23106, 9. Indicator, 10. Empty, 11. Empty, 12. Empty
New culture
by Pauline, Audrey
New liquid culture of:
- K1493504
2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Audrey, Coralie, Pauline
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.