Difference between revisions of "Team:Paris Saclay/Notebook/July/7"

(Tuesday 7th July)
(Electrophoresis)
 
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{{Team:Paris_Saclay/notebook_header}}
 
=Tuesday 7th July=
 
=Tuesday 7th July=
==Back in Lab==
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==Labwork==
====Verification of our plasmids====
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===Verification of our plasmids===
 
''by Coralie, Johan, Seong Koo, Audrey, Pauline''
 
''by Coralie, Johan, Seong Koo, Audrey, Pauline''
  
=====Biobricks verification:=====  
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====Biobricks verification====
 
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*K115017
 
*K115017
 
*C0040
 
*C0040
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*J23106
 
*J23106
 
*I13504
 
*I13504
*K1493504
 
 
=====Extraction=====
 
  
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====Extraction====
 
With the Nucleospin Kit from Macherey Nagel
 
With the Nucleospin Kit from Macherey Nagel
  
=====Digestion by NotI: =====
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====Digestion by NotI====
 
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Stock solution preparation :  
 
Stock solution preparation :  
 
* 124µL H2O
 
* 124µL H2O
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Incubation 1h30 at 37°C
 
Incubation 1h30 at 37°C
  
=====Electrophoresis=====
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====Electrophoresis====
 
''by Johan, Audrey, Pauline''
 
''by Johan, Audrey, Pauline''
  
 
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET
 
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET
 
Migration 0,06A 80V
 
Migration 0,06A 80V
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[[File:ParisSaclay_070715-digestion_vérif_plasmides.jpg|300px|center]]
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<html>
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<p><i>Verification, from left to right, 1. <a href="https://2015.igem.org/File:Paris_Saclay-Ladder.jpg" target="_blank">DNA Ladder</a>, 2. BBa_J23117, 3. BBa_J23101, 4. BBa_I13602, 5. BBa_I13504, 6. BBa_K115017, 7. BBa_C0040, 8. BBa_J23106, 9. Indicator, 10. Empty, 11. Empty, 12. Empty</i></p></html>
  
 
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===New culture===
====New culture====
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''by Pauline, Audrey''
 
''by Pauline, Audrey''
  
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2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.  
 
2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.  
 
We incubate cultures at 37°C, ON.
 
We incubate cultures at 37°C, ON.
 
  
 
'''Members present:'''
 
'''Members present:'''
 
* Instructors and advisors: Alice.
 
* Instructors and advisors: Alice.
 
* Students: Johan, Seong Koo, Audrey, Coralie, Pauline
 
* Students: Johan, Seong Koo, Audrey, Coralie, Pauline
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 21:10, 18 September 2015


Tuesday 7th July

Labwork

Verification of our plasmids

by Coralie, Johan, Seong Koo, Audrey, Pauline

Biobricks verification

  • K115017
  • C0040
  • I13602
  • J23101
  • J23117
  • J23106
  • I13504

Extraction

With the Nucleospin Kit from Macherey Nagel

Digestion by NotI

Stock solution preparation :

  • 124µL H2O
  • 16µL buffer FastDigest 10x
  • 4µL NotI

We use 18µL from this solution with 2µL plasmid solution

Incubation 1h30 at 37°C

Electrophoresis

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

ParisSaclay 070715-digestion vérif plasmides.jpg

Verification, from left to right, 1. DNA Ladder, 2. BBa_J23117, 3. BBa_J23101, 4. BBa_I13602, 5. BBa_I13504, 6. BBa_K115017, 7. BBa_C0040, 8. BBa_J23106, 9. Indicator, 10. Empty, 11. Empty, 12. Empty

New culture

by Pauline, Audrey

New liquid culture of:

  • K1493504

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Audrey, Coralie, Pauline

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