Difference between revisions of "Team:Minnesota/Safety"

 
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{{Minnesota}}
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In order to comply with state and federal law, all team members that worked in the lab took the following courses: Introduction to Research Safety, Chemical Safety, Chemical Waste Management, and Globally Harmonized System of Classification and Labelling of Chemicals. The two microorganisms we used were CEN.PK2 cells of <i>Saccharomyces cerevisiae</i> and JM109 cells of <i>Escherichia coli</i>. Both of these chassis are biosafety level 1 organisms in addition the former is an auxotrophic strain. We choose to have yeast as our chassis because of its general public acceptance and for its ability to readily homologous recombine sections of DNA into plasmids. In addition we followed state and federal laws regarding working with biosafety level 1 organisms such as autoclaving disposable lab supplies that touched these organisms or any DNA before it was thrown out and spraying our benchtop with 70% ethanol when the contaminated media fell o
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
  
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                  <li
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                class='selected'       ><a href="/Team:Minnesota/Safety">Page              </a></li>
 +
              <li
 +
                class='new'       ><a href="/wiki/index.php?title=Talk:Team:Minnesota/Safety&amp;action=edit&amp;redlink=1">Discussion              </a></li>
 +
              <li
 +
                    ><a href="/wiki/index.php?title=Team:Minnesota/Safety&amp;action=edit">View source              </a></li>
 +
              <li
 +
                    ><a href="/wiki/index.php?title=Team:Minnesota/Safety&amp;action=history">History              </a></li>
 +
              <li style='color:white;cursor:default'>teams</li>
 +
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 +
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<h4>Safe Project Design</h4>
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 +
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<title>Team:Minnesota - Template</title>
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 +
      $(".mainContentTextBanner").text("Welcome to the University of Minnesota iGEM team website! Please take some time to explore around by hovering over the icons on the right for quick blurbs or navigate using the buttons to the left. We hope you enjoy learning about us and what we do!");
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/*
 +
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 +
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 +
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$("#SafetyU").click(
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      var suString = '<h2>Safety at the University of Minnesota</h2><br>Biosafety rules for the University of Minnesota are described in the Biosafety Manual, which is produced by the Department of Environmental Health and Safety (DEHS). The Biosafety Manual is available online <a href="http://www.dehs.umn.edu/bio_pracprin.htm">here</a>.<br><br>';
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 +
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 +
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 +
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 +
      $(this).attr("class","current2");
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 +
 +
);
 +
 +
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 +
    function(){
 +
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 +
      $(this).attr("class","current2");
 +
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 +
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 +
 +
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 +
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 +
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 +
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 +
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 +
*/
 +
 +
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 +
 +
 +
 +
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 +
 +
<style type="text/css">
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
 +
 +
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 +
 +
 +
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 +
 +
 +
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 +
 +
<div id="MainBannerImage">
 +
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 +
<div id="MainBanner">
 +
</div>
 +
 +
 +
<div id="SideBarAll">
 +
<div class="sideNavBar2">
 
<ul>
 
<ul>
−
<li>Choosing a non-pathogenic chassis</li>
+
<li><a href='2015.igem.org/Team:Minnesota'>HOME</a></li>
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
<li><a href='https://2015.igem.org/Team:Minnesota/Team'>TEAM</a></li>
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
<li><a href='https://2015.igem.org/Team:Minnesota/Project'>PROJECT</a></li>
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
<li><a href='https://2015.igem.org/Team:Minnesota/Software'>SOFTWARE</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Practices'>PRACTICES</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Notebook'>NOTEBOOK</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Attributions'>ATTRIBUTIONS</a></li>
 +
<li><a class="current" href='https://2015.igem.org/Team:Minnesota/Safety'>SAFETY</a></li>
 +
<li><a href='https://2015.igem.org/Team:Minnesota/Sponsor'>SPONSOR</a></li>
 
</ul>
 
</ul>
 +
</div>
  
−
<h4>Safe Lab Work</h4>
 
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
</div> <!--End sideBarAll-->
  
−
<h4>Safe Shipment</h4>
+
<div id="MainBoxAll">
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
  
 +
<div id="MainBoxContent" style="background-color:white;">
  
 +
<div id="Safety">
 +
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<a href="#" id="SafetyU">Safety at the U</a>
 +
<a href="#RS" target="boxInBox" id="SafetyResearcher">Researcher Safety</a>
 +
<a href="#" id="SafetyPublic">Public Safety</a>
 +
<a href="#" id="SafetyEnvironment">Environmental Concerns</a>
 +
-->
 +
 +
 +
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 +
 +
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<div class="ScrollDiv"> <!---START HERE--->
 +
 +
 +
 +
<h1>Safety Information</h1>
 +
<br>
 +
As part of our safety training everyone who worked in the lab had to take the following online training tutorials DEHS Introduction: Research Safety, DEHS Chemical Safety, DEHS Chemical Waste Management, and DEHS Globally Harmonized System of Classification and Labeling of Chemicals
 +
<br>
 +
To minimize our risk as well as the risk to the environment we chose to work with two non-pathogenic species: Saccharomyces cerevisiae CEN.PK2 and  Escherichia coli JM109. In addition to being non-pathogenic, the yeast strain has the additional safeguard in that it is an auxotrophic species unable to synthesize tryptophan, uracil, leucine, and histidine. We always wore nitrile gloves when working on the benchtop, and we autoclaved anything biosafety level 1 organisms could have touched before discarding it as directed by the lab biosafety officer, Chris Flynn. We wore gloves whenever we were working with ethidium bromide, We wore lab coats, gloves, and a face mask whenever we had the UV light on to excise DNA from an electrophoresis gel, and we wore gloves while working in a fume hood when we used hexane.
 +
<br>
 +
For our sequences we chose to use sequences all from Biosafety level 1 organisms. In particular we chose to use the Thosea asigna (BS1) viral 2A sequences over the Foot-and-mouth-Disease (BS4) viral 2A sequences just to be on the safe side.
 +
</div><!--END HERE!-->
 +
 +
 +
 +
 +
 +
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 +
 +
 +
 +
<div id="MainBoxOtherLinks">
 +
<p class="otherLinks">
 +
<a href="https://2015.igem.org/Main_Page">iGEM Home</a> | <a href="https://2015.igem.org/Team:Minnesota/Judges" style="color:red;">iGEM Judge-Click Here!</a> | <a href="https://igem.org/Team.cgi?id=1187">Team Minnesota Info</a> |
 +
<a href="http://www1.umn.edu/twincities/index.html">University of Minnesota Home</a> |  <a href="C:\Documents and Settings\aj\Desktop\IGEM webpage/Contact.html">Contact Us!</a>
 +
</p>
 +
 +
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Retrieved from "<a href="C:\Documents and Settings\aj\Desktop\IGEM webpage/Safety.html">C:\Documents and Settings\aj\Desktop\IGEM webpage/Safety.html</a>"</div>
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Latest revision as of 21:20, 18 September 2015

Team:Minnesota/Safety - 2015.igem.org

 

Team:Minnesota/Safety

From 2015.igem.org

Team:Minnesota - Main Style Template

Team:Minnesota - Template

Safety Information


As part of our safety training everyone who worked in the lab had to take the following online training tutorials DEHS Introduction: Research Safety, DEHS Chemical Safety, DEHS Chemical Waste Management, and DEHS Globally Harmonized System of Classification and Labeling of Chemicals
To minimize our risk as well as the risk to the environment we chose to work with two non-pathogenic species: Saccharomyces cerevisiae CEN.PK2 and Escherichia coli JM109. In addition to being non-pathogenic, the yeast strain has the additional safeguard in that it is an auxotrophic species unable to synthesize tryptophan, uracil, leucine, and histidine. We always wore nitrile gloves when working on the benchtop, and we autoclaved anything biosafety level 1 organisms could have touched before discarding it as directed by the lab biosafety officer, Chris Flynn. We wore gloves whenever we were working with ethidium bromide, We wore lab coats, gloves, and a face mask whenever we had the UV light on to excise DNA from an electrophoresis gel, and we wore gloves while working in a fume hood when we used hexane.
For our sequences we chose to use sequences all from Biosafety level 1 organisms. In particular we chose to use the Thosea asigna (BS1) viral 2A sequences over the Foot-and-mouth-Disease (BS4) viral 2A sequences just to be on the safe side.

Retrieved from "C:\Documents and Settings\aj\Desktop\IGEM webpage/Safety.html"