Difference between revisions of "Team:Berlin/notebook/protocols"

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        <a href="https://2015.igem.org/Team:Berlin/notebook" class="sub-link-project"> 1. What's the problem?</a><br/><br/>
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        <a href="https://2015.igem.org/Team:Berlin/notebook" class="sub-link-project"> 1. Lab Summary</a><br/><br/>
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      <a href="https://2015.igem.org/Team:Berlin/notebook/summary" class="sub-link-project"> 2. Lab Summary?</a><br/><br/>
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        <a href="https://2015.igem.org/Team:Berlin/notebook/protocols" class="sub-link-project"> 2. Protocols</a><br/><br/>
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        <a href="https://2015.igem.org/Team:Berlin/notebook/protocols" class="sub-link-project"> 3. Protocols</a><br/><br/>
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<a href="https://2015.igem.org/Team:Berlin/Description" class="sub-link-project"> 3. Debugging</a>
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    <div class="col-xs-11 col-sm-9 blog-text" style="margin-bottom:40px;">
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      <div class="project-headline-float">
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      <h4 class="blue-text project-headline"><font face="Arial">2. Protocols</font></h4>
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      <p>
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<object data="Team_Berlin_15_agarose_gel_electrophoresis.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/b/b2/Team_Berlin_15_agarose_gel_electrophoresis.pdf">Agarose Gel Electrophoresis</a>
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</object>
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<object data="Team_Berlin_Cellulose_SOP.pdf.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="
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https://static.igem.org/mediawiki/2015/b/b2/Team_Berlin_Cellulose_SOP.pdf">Cellulose SOPs</a>
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</object><br/><br/>
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<object data="Team_Berlin_Colony_PCR.pdf" type="application/pdf" style="width:100%;height:300px">
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<a href="https://static.igem.org/mediawiki/2015/f/fe/Team_Berlin_Colony_PCR.pdf">Colony PCR</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_15_competent_cells.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/c/c4/Team_Berlin_15_competent_cells.pdf">Competent Cells</a>
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</object>
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<object data="Team_Berlin_Flagella_Expression_Isolation.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/6/68/Team_Berlin_Flagella_Expression_Isolation.pdf">Flagella Expression and Isolation</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_Gibson_Assembly.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/8/8e/Team_Berlin_Gibson_Assembly.pdf">Gibson Assembly</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_Isolation_of_plasmid_DNA.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/a/ad/Team_Berlin_Isolation_of_plasmid_DNA.pdf">Isolation of plasmid DNA</a>
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</object>
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<object data="Team_Berlin_Ligation_based_Cloning.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="
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https://static.igem.org/mediawiki/2015/5/5d/Team_Berlin_Ligation_based_Cloning.pdf">Ligation based Cloning</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_Media_Cultivation.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/0/0a/Team_Berlin_Media_Cultivation.pdf">Media for Cultivation</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_Motility_Essay.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/9/92/Team_Berlin_Motility_Essay.pdf">Motility Essay</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_Overexpression_GFP.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/1/16/Team_Berlin_Overexpression_GFP.pdf">Overexpression of GFP</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_PCR_Amplification.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/7/74/Team_Berlin_PCR_Amplification.pdf">PCR Amplification</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_Protein_Concentration_Determination.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="https://static.igem.org/mediawiki/2015/9/93/Team_Berlin_Protein_Concentration_Determination.pdf">Protein Concentration Determination</a>
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</object>
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<br/><br/>
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<object data="Team_Berlin_Protein_Purification.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="
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https://static.igem.org/mediawiki/2015/4/40/Team_Berlin_Protein_Purification.pdf">Protein Purification</a>
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</object> <br/><br/>
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<object data="Team_Berlin_SDS_Polyacrylamide_gel" type="application/pdf" style="width:100%;height:300px">
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  <a href="
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https://static.igem.org/mediawiki/2015/0/04/Team_Berlin_SDS_Polyacrylamide_gel.pdf">SDS Polyacrylamide Gel</a>
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</object> <br/><br/>
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<object data="Team_Berlin_Sequenzierung.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="
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https://static.igem.org/mediawiki/2015/9/94/Team_Berlin_Sequenzierung.pdf">Sequencing</a>
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</object><br/><br/>
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<object data="Team_Berlin_Transformation.pdf" type="application/pdf" style="width:100%;height:300px">
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  <a href="
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https://static.igem.org/mediawiki/2015/c/c2/Team_Berlin_Transformation.pdf">Transformation</a>
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</section>  
 
</section>  

Latest revision as of 21:24, 18 September 2015

1. Lab Summary

2. Protocols

3. Debugging




2. Protocols

Agarose Gel Electrophoresis

Cellulose SOPs

Colony PCR

Competent Cells

Flagella Expression and Isolation

Gibson Assembly

Isolation of plasmid DNA

Ligation based Cloning

Media for Cultivation

Motility Essay

Overexpression of GFP

PCR Amplification

Protein Concentration Determination

Protein Purification

SDS Polyacrylamide Gel

Sequencing

Transformation

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