Difference between revisions of "Team:LZU-China/Parts"

 
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           <a href="https://2015.igem.org/Team:LZU-China/chz_project">Project</a>
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           <a href="https://2015.igem.org/Team:LZU-China/project">Project</a>
 
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           <a href="https://2015.igem.org/Team:LZU-China/Practices">HumanPractice</a>
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           <a href="https://2015.igem.org/Team:LZU-China/Practices">Human Practices</a>
 
         </li>
 
         </li>
 
         <li>
 
         <li>
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           <a href="https://2015.igem.org/Team:LZU-China/Attributions">Team</a>
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           <a href="https://2015.igem.org/Team:LZU-China/Attributions">Attributions</a>
 
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         </li>
 
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         <li>
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           <a href="https://2015.igem.org/Team:LZU-China/chz_safety">Safety</a>
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           <a href="https://2015.igem.org/Team:LZU-China/safety">Safety</a>
 
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           <a href="https://2015.igem.org/Team:LZU-China/chz_notes">Notes</a>
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           <a href="https://2015.igem.org/Team:LZU-China/notes">Notes</a>
 
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</header>
 
</header>
  
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<div class="content inorder">
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  <div class="row">
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  <div id="index-banner" class="parallax-container inorder">
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     <div class="col s12 bgbanner">
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     <div class="section no-pad-bot">
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       <div class="container">
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         <div class="col s12 ">
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         <br><br>
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          <h5 class="center-align">&nbsp;</h5>
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        <h1 class="header center teal-text text-lighten-2"> <span class="cyan-text text-accent-2">Part list</span></h1>
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          <h2 class="center-align white-text">Parts List</h2>
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        <div class="row center">
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          <h5 class="center-align white-text"></h5>
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          <h3 class="center-align">&nbsp;</h3>
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         </div>
 
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        <br><br>
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       </div>
 
       </div>
 
     </div>
 
     </div>
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    <div class="parallax"><img class="bgimagex"  src="https://static.igem.org/mediawiki/2015/8/80/LZU_China_part_banner.png" alt="Unsplashed background img 2"></div>
 
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   <p>RibB is our basic gene. Every part basic on it.</p>
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   <strong><h6><p>RibB is our basic gene. Every part basic on it.</p></h6></strong>
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  <p>
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    The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
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This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
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Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)
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This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
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   <div class="row">
 
   <div class="row">
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                 </div>
 
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                 <div class="card-content">
 
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                   <p>Figure 1:Constitutive parts </p>
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                   <p>Figure 1:Constitutive parts <br/>K1755005 K1755006 K1755007 K1755008 and K1755009</p>
 
                 </div>
 
                 </div>
 
                 <!--<div class="card-action">
 
                 <!--<div class="card-action">
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               </div></p>
 
               </div></p>
 
           </div>
 
           </div>
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  <p>
 +
    The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
 +
This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
 +
Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB).
 +
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis.
 +
  </p>
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  <div class="row">
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            <p> <div class="col s12 m5 l5">
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                <div class="card">
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                <div class="card-image">
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                <img class="left-align" src="https://static.igem.org/mediawiki/parts/0/04/K1755301ceshi.png">
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                  <span class="card-title"></span>
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                </div>
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                <div class="card-content">
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                  <p>Figure 3:Concentration of riboflavin vs Cu ions</p>
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                </div>
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                <!--<div class="card-action">
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                  <a href="#">This is a link</a>
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                </div>-->
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              </div>
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              </div>
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              <div class="col s12 m5 l5">
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                <div class="card">
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                <div class="card-image">
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                  <img class="right-align" src="https://static.igem.org/mediawiki/parts/b/b5/K1755301time.png">
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                  <span class="card-title"></span>
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                </div>
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                <div class="card-content">
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                  <p>Figure 4:Riboflavin concentration when the CuSO<sub>4</sub>  in 5mg/L</p>
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                </div>
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                <!--<div class="card-action">
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                  <a href="#">This is a link</a>
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                </div>-->
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              </div>
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        </div></p>
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    </div>
  
 
       <P> We construct many parts as the following list.</P>
 
       <P> We construct many parts as the following list.</P>
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           <h5 class="white-text">LZU-China 2015</h5>
 
           <h5 class="white-text">LZU-China 2015</h5>
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           <p class="grey-text text-lighten-4">222, TianShui South Road, Lanzhou, China, 730000</p>
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           <p class="grey-text text-lighten-4">NO.222, TianShui South Road, Lanzhou, China, 730000</p>
 
           <h6 class="white-text">Copyright 2015 Lanzhou University</h6>
 
           <h6 class="white-text">Copyright 2015 Lanzhou University</h6>
  
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             <li><a class="white-text" href="http://en.lzu.edu.cn/">Educational Administration Office, LZU</a></li>
 
             <li><a class="white-text" href="http://en.lzu.edu.cn/">Educational Administration Office, LZU</a></li>
 
             <li><a class="white-text" href="http://chc.lzu.edu.cn/en/">Cuiying Honors College, LZU</a></li>
 
             <li><a class="white-text" href="http://chc.lzu.edu.cn/en/">Cuiying Honors College, LZU</a></li>
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             <li><a class="white-text" href="http://lifesc.lzu.edu.cn/">School of life, LZU</a></li>
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             <li><a class="white-text" href="http://lifesc.lzu.edu.cn/">School of Life Sciences,LZU</a></li>
 
           </ul>
 
           </ul>
 
         </div>
 
         </div>

Latest revision as of 21:36, 18 September 2015

Team:LZU 2015



Part list



Unsplashed background img 2

RibB is our basic gene. Every part basic on it.

Figure 1:Constitutive parts
K1755005 K1755006 K1755007 K1755008 and K1755009

Figure 2:Our parts working in MFC

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB). This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis.

Figure 3:Concentration of riboflavin vs Cu ions

Figure 4:Riboflavin concentration when the CuSO4 in 5mg/L

We construct many parts as the following list.

Parts list
Part ID Characterization Details
K1755001 ribB coding sequence more information
K1755002 ribB + B0014(terminator) more information
K1755003 B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator) more information
K1755004 K525998(PT7+RBS) + ribB + B0014(terminator) more information
K1755005 K608003(strong promoter+medium RBS) + ribB + B0014(terminator) more information
K1755006 K608006(medium promoter+medium RBS) + ribB + B0014(terminator) more information
K1755007 B608002(strong promoter+strong RBS)+ ribB + B0014(terminator) more information
K1755008 K608004(strong Promoter and weak RBS)+ribB+B0014(Double terminator) more information
K1755009 K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator) more information
K1755301 MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator) more information
K1755302 K190024(Copper sensor) + ribB + B0014(terminator) more information
K1755303 K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator) more information
K1755305 K346002(MerO) + B0032(RBS) + ribB + B0014(terminator) more information
K1755024 K911003+ B0032(RBS) + ribB + B0014(terminator) more information