Difference between revisions of "Team:LZU-China/Parts"
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| − | <a href="https://2015.igem.org/Team:LZU-China/Practices"> | + | <a href="https://2015.igem.org/Team:LZU-China/Practices">Human Practices</a> |
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| − | <a href="https://2015.igem.org/Team:LZU-China/ | + | <a href="https://2015.igem.org/Team:LZU-China/safety">Safety</a> |
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<li> | <li> | ||
| − | <a href="https://2015.igem.org/Team:LZU-China/ | + | <a href="https://2015.igem.org/Team:LZU-China/notes">Notes</a> |
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| − | + | <div id="index-banner" class="parallax-container inorder"> | |
| − | <div class=" | + | <div class="section no-pad-bot"> |
| − | <div class=" | + | <div class="container"> |
| − | < | + | <br><br> |
| − | + | <h1 class="header center teal-text text-lighten-2"> <span class="cyan-text text-accent-2">Part list</span></h1> | |
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| + | <div class="parallax"><img class="bgimagex" src="https://static.igem.org/mediawiki/2015/8/80/LZU_China_part_banner.png" alt="Unsplashed background img 2"></div> | ||
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The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. | The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. | ||
This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. | This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. | ||
| − | Proteins known to be involved in this subpathway in this organism are | + | Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB). |
| − | This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of | + | This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis. |
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<h5 class="white-text">LZU-China 2015</h5> | <h5 class="white-text">LZU-China 2015</h5> | ||
| − | <p class="grey-text text-lighten-4">222, TianShui South Road, Lanzhou, China, 730000</p> | + | <p class="grey-text text-lighten-4">NO.222, TianShui South Road, Lanzhou, China, 730000</p> |
<h6 class="white-text">Copyright 2015 Lanzhou University</h6> | <h6 class="white-text">Copyright 2015 Lanzhou University</h6> | ||
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<li><a class="white-text" href="http://en.lzu.edu.cn/">Educational Administration Office, LZU</a></li> | <li><a class="white-text" href="http://en.lzu.edu.cn/">Educational Administration Office, LZU</a></li> | ||
<li><a class="white-text" href="http://chc.lzu.edu.cn/en/">Cuiying Honors College, LZU</a></li> | <li><a class="white-text" href="http://chc.lzu.edu.cn/en/">Cuiying Honors College, LZU</a></li> | ||
| − | <li><a class="white-text" href="http://lifesc.lzu.edu.cn/">School of | + | <li><a class="white-text" href="http://lifesc.lzu.edu.cn/">School of Life Sciences,LZU</a></li> |
</ul> | </ul> | ||
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Latest revision as of 21:36, 18 September 2015
Part list
RibB is our basic gene. Every part basic on it.
Figure 1:Constitutive parts
K1755005 K1755006 K1755007 K1755008 and K1755009
Figure 2:Our parts working in MFC
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB). This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis.
Figure 3:Concentration of riboflavin vs Cu ions
Figure 4:Riboflavin concentration when the CuSO4 in 5mg/L
We construct many parts as the following list.
Parts list |
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|---|---|---|
| Part ID | Characterization | Details |
| K1755001 | ribB coding sequence | more information |
| K1755002 | ribB + B0014(terminator) | more information |
| K1755003 | B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator) | more information |
| K1755004 | K525998(PT7+RBS) + ribB + B0014(terminator) | more information |
| K1755005 | K608003(strong promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755006 | K608006(medium promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755007 | B608002(strong promoter+strong RBS)+ ribB + B0014(terminator) | more information |
| K1755008 | K608004(strong Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755009 | K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755301 | MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755302 | K190024(Copper sensor) + ribB + B0014(terminator) | more information |
| K1755303 | K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755305 | K346002(MerO) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755024 | K911003+ B0032(RBS) + ribB + B0014(terminator) | more information |