Difference between revisions of "Team:LZU-China/Parts"
Ritianjiang (Talk | contribs) |
|||
| (9 intermediate revisions by 2 users not shown) | |||
| Line 71: | Line 71: | ||
</header> | </header> | ||
| − | <div | + | |
| − | + | <div id="index-banner" class="parallax-container inorder"> | |
| − | <div class=" | + | <div class="section no-pad-bot"> |
| − | <div class=" | + | <div class="container"> |
| − | < | + | <br><br> |
| − | + | <h1 class="header center teal-text text-lighten-2"> <span class="cyan-text text-accent-2">Part list</span></h1> | |
| − | + | <div class="row center"> | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
| + | |||
| + | <br><br> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
| + | <div class="parallax"><img class="bgimagex" src="https://static.igem.org/mediawiki/2015/8/80/LZU_China_part_banner.png" alt="Unsplashed background img 2"></div> | ||
</div> | </div> | ||
| − | |||
| Line 129: | Line 130: | ||
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. | The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. | ||
This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. | This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. | ||
| − | Proteins known to be involved in this subpathway in this organism are | + | Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB). |
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis. | This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis. | ||
</p> | </p> | ||
Latest revision as of 21:36, 18 September 2015
Part list
RibB is our basic gene. Every part basic on it.
Figure 1:Constitutive parts
K1755005 K1755006 K1755007 K1755008 and K1755009
Figure 2:Our parts working in MFC
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB). This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of cofactor biosynthesis.
Figure 3:Concentration of riboflavin vs Cu ions
Figure 4:Riboflavin concentration when the CuSO4 in 5mg/L
We construct many parts as the following list.
Parts list |
||
|---|---|---|
| Part ID | Characterization | Details |
| K1755001 | ribB coding sequence | more information |
| K1755002 | ribB + B0014(terminator) | more information |
| K1755003 | B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator) | more information |
| K1755004 | K525998(PT7+RBS) + ribB + B0014(terminator) | more information |
| K1755005 | K608003(strong promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755006 | K608006(medium promoter+medium RBS) + ribB + B0014(terminator) | more information |
| K1755007 | B608002(strong promoter+strong RBS)+ ribB + B0014(terminator) | more information |
| K1755008 | K608004(strong Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755009 | K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator) | more information |
| K1755301 | MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755302 | K190024(Copper sensor) + ribB + B0014(terminator) | more information |
| K1755303 | K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755305 | K346002(MerO) + B0032(RBS) + ribB + B0014(terminator) | more information |
| K1755024 | K911003+ B0032(RBS) + ribB + B0014(terminator) | more information |